35 results about "Galactomyces" patented technology

The invention discloses two lactobacillus separation strains with anti-inflammatory activity and beneficial properties, which are Lactobacillus sakei GMNL-76 and Lactobacillus reuteri GMNL-89. The two lactobacillus separation strains are respectively preserved in the Bioresouce Collection and Research Center (BCRC) of the Food Industry Research and Development Institute (FIRDI) by preservation numbers of BCRC 910355 and BCRC 910340 and preserved in the China Center for Type Culture Collection (CCTCC) by the preservation numbers of CCTCC M 207153 and CCTCC M 207154. The two lactobacillus separation strains and subculture offspring thereof can be used for preparing various food products and pharmaceutical composition for curing and / or mitigating diseases (such as rheumatoid arthritis) relevant to inflammation.

The invention provides the application of lactobacillus in frozen meat preservation and simultaneously provides a new bacterial strain A71 of Lactobacillus plantarum with a preservation number of CGMCC No.2228. The bacterial strain has an excellent culturing characteristic, a fermenting liquid of the bacterial strain has an excellent bacterial restraining capacity and can be used in the field of food preservation, in particular to the frozen meat preservation.

The invention discloses Lactobacillus brevis, freeze-dried powder of the Lactobacillus brevis and application of the freeze-dried powder, and belongs to the technical field of microorganisms. The name of the Lactobacillus brevis is Lactobacillus brevis ZLB004; the collection number is CGMCC No.5760; and the Lactobacillus brevis is an acid-resistant, cholate-resistant, and high-temperature-resistant strain with a good antibacterial property. The bacterial powder of the Lactobacillus brevis is obtained by the following steps of: performing fermentation culture on the Lactobacillus brevis, centrifuging, adding bacterial mud into a cryoprotectant, and freeze-drying. The bacterial powder of the Lactobacillus brevis, which serves as an animal feed additive, can improve the daily gain of growingpigs, reduce the feed-weight ratio, increase the content of viable lactic acid bacteria in intestines and reduce the content of Escherichia coli, and has the effects of reducing animal stress and promoting the health of organisms.

The invention relates to a lactobacillus pentosus strain and a fermentation product of the same. The preservation number of the lactobacillus pentosus LPEM818 in the invention is CGMCC No. 4583 in the China General Microbiological Culture Collection Center. The fermentation product of the lactobacillus pentosus in the invention can inhibit common pathogenic escherichia coli, pseudomonas aeruginosa and staphylococcus aureus in food, and particularly has a strong inhibiting effect on listeria monocytogenes which can cause serious food poisoning. In addition, the metabolite of the strain also can inhibit the growth of aspergillus flavus and cotton and tomato blight pathogens. Consequently, the lactobacillus pentosus strain and the fermentation product thereof play an active role in the fields of food preservation and plant protection.

The invention provides a preparation method of a microorganism flocculating agent by utilizing novel microorganism flocculating agent producing bacteria, i.e., Galactomyces geotrichum in CGMCC (China General Microbiological Culture Collection Center) NO.2535 and a microorganism flocculating agent prepared by using the method. The preparation method of the microorganism flocculating agent by utilizing the microorganism flocculating agent producing bacteria is simple and easy and is short in fermenting time and low in production cost; the produced flocculating agent has a wide applicable scope, a small using amount but high flocculating efficiency; and the microorganism flocculating agent which is prepared by extracting has no toxin or harm and no secondary pollution, is biodegradable and suitable for large-scale spread and application, and has a wide application prospect.

The invention discloses a method for preparing lysine-rich fermented soybean meal, which is simple and can effectively improve the content of nutrients of the soybean meal. The method is technically characterized by comprising the following steps: (1) preparing strains: a, inoculating the brevibacterium flavum into the brevibacterium flavum liquid culture medium, and vibrating and culturing at 28-37 DEG C to obtain the first-level brevibacterium flavum strain when the OD590 value of the brevibacterium flavum liquid is 0.25-0.30; b, the inoculating the saccharomycetes into the saccharomycetes liquid culture medium, and vibrating and culturing at 28-37 DEG C to obtain the first-level saccharomycetes strain when the OD590 value of the saccharomycetes liquid is 0.25-0.30; and c, inoculating the lactobacillus into the lactobacillus liquid culture medium, and vibrating and culturing at 30-37 DEG C to obtain the first-level lactobacillus strain when the OD590 value of the lactobacillus liquid is 0.25-0.30; (2) preparing a fermentation substrate; and (3) inoculating the fermentation substrate. The method for preparing the lysine-rich fermented soybean meal belongs to the technical field of feed preparation.

The invention provides a preparation method of anti-oxidization probiotics fermented milk. One or a mixture of two of lactobacillus casei and lactobacillus plantarum lyophilized bacterium powder, and saccharomyces cerevisiae boulardii lyophilized bacterium powder are added into degreased powder; the anti-oxidization probiotics fermented milk prepared by the invention keeps nutrient components in milk and the number of living bacteria of lactobacillus and saccharomyces cerevisiae boulardii in the fermented milk is high; probiotic functions can be realized when a consumer intakes the milk; when the saccharomyces cerevisiae boulardii exists, the survival rate of the lactobacillus in the fermented milk can be improved and the shelf life of the fermented milk is prolonged (from conventional three weeks to four weeks); the saccharomyces cerevisiae boulardii can be used for preventing and treating diarrhea caused by a plurality of types of reasons; and furthermore, an anti-oxidation peptide obtained by hydrolyzing lactoprotein through the lactobacillus has an anti-ageing function and can be used for preventing a series of diseases including senile dementia and the like caused by an organism oxidization effect.

The invention relates to an acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure and belongs to the technical field of environmental engineering. The bacteria are cylindrical single-celled bacteria, the arthrospore size of the bacteria is 6<-12>mu m *3<-6>mu m, the bacterial colony is diffused on a plane, the bacteria grow quickly, the bacteria are flat, high in center and flock-like, the liquid culturing the bacteria is turbid, the precipitate is loose, the reproductive mode of the bacteria is fissiparism, and the formed arthrospores are single ones or connected into a chain. The bacteria can grow normally in an extremely acidic environment in which the pH value is 2.5, the most suitable pH is 2.5 to 6, and according to morphological and physiological biochemical characters and molecular biological assay, the bacterial strain belongs to Galactomyces geotrichum and the collection number of the bacterial strain is CGMCC No.4298. The strain in a pig manure and urban sludge bioleaching environment can quickly use water soluble small molecular weight organic matters or simple organic acids as a carbon source and anenergy source, so the bioleaching efficiency is improved, the rate of removal of heavy metals from the pig manure and urban sludge reaches over 90 percent, and the dehydration rates for the pig manure and urban sludge are 48 and 80 percent respectively.

The invention discloses a culture medium for separating and screening lactobacillus fermentum in human intestinal canals and application thereof, and belongs to the technical field of microorganism. The culture medium inhibits the growth of common lactobacillus and other lactic acid bacteria in the human intestinal canals and ensures growth of lactobacillus fermentum in the culture medium on the basis of a traditional lactobacillus screened culture medium LAMVAB culture medium by replacing a carbon source and adding streptomycin with the concentration being 0.256*10-3mg / mL and gentamicin withthe concentration being 6.4*10-6mg / mL. The culture medium solves the problem that the lactobacillus fermentum in the human intestinal canals is difficult to screen, and improves the screening efficiency of the lactobacillus fermentum in the human intestinal canals.

The invention discloses a yeast strain, which is of a galactomyces geotrichum S9 strain. The galactomyces geotrichum S9 strain is collected in China General Microbiological Culture Collection Center on March 5, 2012, the collection and registration number is CCTCC M2012056, the galactomyces geotrichum S9 strain can degrade lincomycin and is derived from a certain metabolite component in a fermentation solution of the strain, and the main action mechanism is that the certain metabolite component generated by the strain can produce action on a certain key chemical bond in the structure of the lincomycin and further degrade the lincomycin into small molecular substances. Simultaneously, the galactomyces geotrichum S9 strain can grow rapidly under the culture conditions described by the invention, industrial production is easy to perform, the galactomyces geotrichum S9 strain has great application and development prospects, and the galactomyces geotrichum S9 strain has high efficiency and practicality when the galactomyces geotrichum S9 is used for treating the fermented leaves of streptomyces lincolnensis.

The invention discloses Galactomyces geotrichum T3d329TF which is obtained by directional induction, cultivation, domestication, selection and separation in natural environment. The Galactomyces geotrichum T3d329TF is preserved in the China Center for Type Culture Collection (CCTCC) on October 21, 2008, and the preserving number is CCTCC NO: M208167. The Galactomyces geotrichum T3d329TF has the action of degrading hexanol and can generate various aroma components simultaneously.

The invention discloses a modern big-pot brewing method of duck-blood glutinous rice and gingko low-alcohol yellow rice wine, and belongs to the technical field of food processing. The modern big-pot brewing method comprises the following steps: polished round-grained rice is taken as the base stock, and mixed with duck-blood glutinous rice and gingko, so that the contents of such active substances as biochrome and ginkgolides in the final yellow rice wine product can be improved; high-temperature alpha-amylase and saccharifying enzyme are respectively adopted for two-step enzymolysis; the raw materials subjected to enzymatic saccharification are mixed with a saccharomyces cerevisiae Chinese yeast for fermentation; after main fermentation, Galactomyces geotrichum KG-1 is added for further fermentation and low-temperature maturation; squeezing, filtering clarification, sterilization and filling are performed to obtain the duck-blood glutinous rice and gingko low-alcohol yellow rice wine. The modern big-pot brewing method for the duck-blood glutinous rice and gingko low-alcohol yellow rice wine has the benefits that as the raw-material high-temperature enzymolysis soaking process is adopted, the processing time is remarkably shortened; as Galactomyces geotrichum KG-1 participates in fermentation of the yellow rice wine, the product alcoholic strength is low, the taste is mellow and refreshing, and the health-care function is enhanced.

The invention discloses lactobacillus brevis capable of improving fermented flavor of fermented dough steamed bun and application thereof, belonging to the field of food microorganisms. According to the lactobacillus brevis provided by the invention, the preservation number is CGMCC NO.14028, and the preservation unit is China General Microbiological Culture Collection Center. The strain is screened from fermented dough, and can be used as a sour dough leavening agent to be used for preparing sour dough and preparing steamed bun. The lactobacillus brevis is fermented for 24h in flour extraction liquor, then the final concentration reaches 8.88(Log CFU / g), deltapH is equal to 0.78, and 61 kinds of volatile flavor compounds are generated, so that the lactobacillus brevis is lactobacillus which is suitable for fermenting dough and endowing the steamed bun with special volatile flavor compound. The lactobacillus brevis is used for the prior fermentation of the steamed bun, and can meet the demands of the traditional fermented steamed buns.

The invention provides a Lactobacillus strain and applications thereof, and belongs to the technical field of microorganisms, wherein the Lactobacillus is preserved in China Center for Type Culture Collection, has the preservation number of CCTCC M 2017450, and has the Latin name of Lactobacillus spicheri IZ. According to the present invention, the colony of the Lactobacillus strain is characterized in that the colony is milk white and round on an MRS agar plate, slightly projects, is wet, has the neat edge, the diameter is 2+ / -1 mm, the cell is rod-like, the suitable growth temperature is 25-35 DEG C, and the strain is a facultative anaerobic strain; the Lactobacillus is used for preparing corn sour spicy, wherein the main flavor of the corn sour spicy can be retained while the miscellaneous bacteria can be significantly inhibited, such that the defective rate of the corn sour spicy can be reduced to 0%; and the Lactobacillus is used for the industrial production of corn sour spicy.

The invention provides the application of lactobacillus in preservation of frozen meat and a new strain A18 of lactobacillus plantarum with the preservation number of CGMCC No.2227. The strain has excellent cultural characteristics with the fermentation broth thereof having excellent bacteriostatic ability and can be applied to the food preservation field, especially the preservation of frozen meat.

The invention relates to CM1 (Galactomyces candidum) capable of degrading cephalosporin antibiotics and belongs to the technical field of microorganisms. The bacterial strain is preserved in the common microorganism center of the Chinese microorganism bacterial strain preservation management committee on November 10, 2017, and the preservation number is CGMCC NO.14633. The bacterial strain is easyto culture, grows quickly, and has the function of degrading cephalosporin antibiotics, and meanwhile the toxicity and inhibition effects of cephalosporin antibiotics on gram-positive bacteria can bedegraded and removed. The CM1 can be applied to fungicide production and treatment of waste of cephalosporin antibiotics production enterprises and residual cephalosporin antibiotics in the environment, and the harm of cephalosporin antibiotics to the environment and people is reduced.

The present invention provides a cosmetic composition containing nicotinamide, galactomyces fermented matter, and lactobacillus fermented matter as active ingredients.

The invention discloses Lactobacillus brevis, freeze-dried powder of the Lactobacillus brevis and application of the freeze-dried powder, and belongs to the technical field of microorganisms. The name of the Lactobacillus brevis is Lactobacillus brevis ZLB004; the collection number is CGMCC No.5760; and the Lactobacillus brevis is an acid-resistant, cholate-resistant, and high-temperature-resistant strain with a good antibacterial property. The bacterial powder of the Lactobacillus brevis is obtained by the following steps of: performing fermentation culture on the Lactobacillus brevis, centrifuging, adding bacterial mud into a cryoprotectant, and freeze-drying. The bacterial powder of the Lactobacillus brevis, which serves as an animal feed additive, can improve the daily gain of growingpigs, reduce the feed-weight ratio, increase the content of viable lactic acid bacteria in intestines and reduce the content of Escherichia coli, and has the effects of reducing animal stress and promoting the health of organisms.

The present invention related to a method for preparing fermented food having suppressive effect against Helicobactor-pylori and food-poisoning bacillus that comprises the steps of inoculating lactic ferments into the food, wherein said lactic ferments is selected from the group consisting of Lactobacillus genus, Bifidobacterium genus, Streptococus genus and the mixture thereof, and said food is selected from the group consisting of grain, fruit, vegetables and their mixture; and incubating said inoculated mixture at 15-35 DEG C. for 12-48 hours.

The invention relates to a recombinant gene of glutamate dehydrogenase, an acquisition method of the recombinant gene, and an application of glutamate dehydrogenase recombinant enzyme. At present, the degradation of higher alcohols in food is poor in specificity, the degradation of the higher alcohols under acidic conditions is restricted, the yield of enzymes in thalli is low, time consuming is long, cost is high and industrial production can not performed. Conserved regions and full-length sequences of glutamate dehydrogenase genes in galactomyces geotrichum T3d329TF strain which are capable of efficiently degrading the higher alcohols under the acidic conditions are subjected to cloning and expression, and recombinant enzyme activity verification is performed. The recombinant enzyme capable of degrading n-hexyl alcohol and isopentanol under the acidic conditions is used as a catalyst for directionally reducing the content of higher alcohols in foods, especially in acidic foods, and the application is safe and environmentally friendly, high in controllability, low in production cost, and suitable for large-scale industrial production.

The invention provides galactomyces geotrichum P14, an application method thereof, and a microbial inoculum prepared from the same, which belong to the technical field of micro-biofertilizer, and aim to realize high-efficiency phosphorus solubilization from the biological aspect by virtue of screened phosphorus solubilizing fungus. According to the microbial inoculum prepared from the galactomyces geotrichum P14, various insoluble phosphates in soil are converted to be in a water-soluble state with high efficiency, and then can be absorbed and utilized by plants. The microbial inoculum is suitable for reddened soil, moisture soil, brown soil and other types of soil, and has the effects of releasing insoluble phosphorus in the soil, preventing soil chemical fixation of phosphorus, improving the utilization efficiency of phosphorus fertilizer, improving the agronomic characters of crops, and increasing yield.

The invention discloses a fermented celery juice rich in active probiotics and a preparation method thereof, and belongs to the field of fruit and vegetable fermentation. The preparation method includes the steps that celery juice, petroselinum crispum juice and water dropwort juice are mixed in specific proportions, and then lactobacillus plantarum is inoculated for fermentation, and the fermented celery juice is prepared. The number of active lactobacillus in the obtained fermented celery juice can reach up to 2.5*109cfu / mL, and after 21 days of storage at low temperature, the number of viable lactobacillus can still be kept above 1.0*108cfu / mL. The celery juice is taken as a main raw material, unique fresh and delicate fragrant natural flavor is well kept, soft and tasty sourness and pleasant aroma are increased after the lactobacillus is fermented, and the obtained product has the double health care functions of celery and the vactobacillus plantarum, in addition, the processing technology is simple, the production cost is low, the celery juice is in line with a consumption concept of low sugar, low addition and natural health of modern consumers, and has a very broad market potential and application prospect.

The invention relates to a preparation method of fermented food which has inhibitory effect on Helicobacter pylori and food poisoning bacillus, which comprises the following steps: inoculating lactic acid fermenting bacteria into food, and culturing at 15-35°C for 12-48 hours , wherein the lactic acid fermenting bacteria are selected from Lactobacillus, Bifidobacterium, Streptococcus or their mixtures, and the food is selected from cereals, fruits, vegetables or their mixtures.

The invention discloses Galactomyces geotrichum T3d329TF which is obtained by directional induction, cultivation, domestication, selection and separation in natural environment. The Galactomyces geotrichum T3d329TF is preserved in the China Center for Type Culture Collection (CCTCC) on October 21, 2008, and the preserving number is CCTCC NO: M208167. The Galactomyces geotrichum T3d329TF has the action of degrading hexanol and can generate various aroma components simultaneously.

The invention discloses a preparation method of natural perfume deriving from porcini. The method includes the following steps that sterilization treatment is conducted on porcini fruiting bodies, then Galactomyces candidum is added for fermenting, and accordingly the natural perfume is obtained; optionally, solvent is added to extract the natural perfume, the extractive is concentrated, and thus natural perfume concentrate is obtained. The natural perfume or the natural perfume concentrate is added into a tobacco product, so that a special aroma is given to the tobacco product, the cigarette flavor is increased obviously, the sweet feeling and delicacy are increased, and the organoleptic quality is improved obviously. In addition, the release amount of tobacco specific N-nitrosamine in smoke of a traditional cigarette and a novel cigarette (heating and non-combustion type cigarette) can be lowered remarkably in the natural perfume.

The invention provides galactomyces geotrichum P14, an application method thereof, and a microbial inoculum prepared from the same, which belong to the technical field of micro-biofertilizer, and aim to realize high-efficiency phosphorus solubilization from the biological aspect by virtue of screened phosphorus solubilizing fungus. According to the microbial inoculum prepared from the galactomyces geotrichum P14, various insoluble phosphates in soil are converted to be in a water-soluble state with high efficiency, and then can be absorbed and utilized by plants. The microbial inoculum is suitable for reddened soil, moisture soil, brown soil and other types of soil, and has the effects of releasing insoluble phosphorus in the soil, preventing soil chemical fixation of phosphorus, improving the utilization efficiency of phosphorus fertilizer, improving the agronomic characters of crops, and increasing yield.

The invention discloses a modern large-pot brewing method of duck blood glutinous ginkgo low-alcohol yellow rice wine, belonging to the technical field of food processing. Using japonica rice as the base material, together with duck blood glutinous rice and ginkgo biloba, can increase the content of active substances such as biochrome and ginkgolide in the final rice wine product, and use high-temperature α-amylase and glucoamylase for two-step enzymatic hydrolysis, respectively, to enzymatically hydrolyze and saccharify The processed raw materials are mixed with Saccharomyces cerevisiae for fermentation. After the main fermentation is completed, the patented strain Geotrichum galactosus is added to continue fermentation and low-temperature aging. After pressing, filtering, clarification, sterilization, and filling, the duck blood glutinous ginkgo low-alcohol yellow rice wine is obtained. The invention has the beneficial effects of adopting the high-temperature enzymatic hydrolysis and soaking process of raw materials, significantly reducing the processing time, and adopting the patent strain Galactomyces geotrichum KG-1 to participate in the rice wine fermentation, the product has low alcohol content, mellow and refreshing taste, and enhanced health care function.