55 results about "Chloride channel" patented technology

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, and its N-oxides, and suitable salts thereof and a component (b) wherein the component (b) is at least one compound or agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and suitable salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, an N-oxide, or a salt thereof, Formula (1) and (b) at least one invertebrate pest control agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

The present invention belongs to the field of insecticides, and relates to a pest-killing and mite-killing composition, which contains an active component A and an active component B, a weight part ratio of the component A to the component B is 1:99-99:1, the active component A is selected from a compound I represented by the following structural formula, the active component B is selected from an organic phosphorus pest / mite killing agent, a nicotine type acetylcholine receptor (nAChR) agonist pest / mite killing agent, an apastia pest / mite killing agent, a proton gradient interference and oxidative phosphorylation affecting uncoupling pest / mite killing agent, a nereistoxin analog pest / mite killing agent, a Ryania receptor regulating pest / mite killing agent, a voltage-dependent sodium ion channel blocking pest / mite killing agent, an ecdysone receptor stimulating pest / mite killing agent, an amino butyric acid (GABA) gated chloride channel antagonism pest / mite killing agent, a microorganism pest / mite killing agent or other pest / mite killing agents, and the active component A has the following structure. The composition of the present invention has advantages of significant synergy, resistance delaying and the like, and can be used for prevention and control of a variety of pests. The compound I is defined in the instruction.

Compositions for reducing dependency and addiction to substances of abuse are provided. Chloride channels such as the GABAA receptors are altered under conditions of dependency and withdrawal such that the electrophysiological properties of the GABAA receptor containing neurons are altered thereby providing a pathophysiological condition resulting in symptoms of dependency and withdrawal such as anxiety. Specifically, under conditions of withdrawal the relative ratio of the a1 receptor subunit decreases relative to the a4 receptor subunit. Endogenous neurosteroid production is also associated with the molecular changes underlying the alterations of GABA-gated chloride channels. Compositions of at least two compounds including at least one inhibitor of neurosteroid production are useful for treating the pathophysiology of addiction, dependency and substance abuse withdrawal.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, and its N-oxides, and suitable salts thereofanda component (b) wherein the component (b) is at least one compound or agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and suitable salts of the foregoing.Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

The present invention provides for methods for diagnosing and treating a motor neuron disease. More specifically, the present invention offers new methods for diagnosing and treating SMA or SMN deficiencies and monitoring treatment. It is possible to identify a subject having a symptom of the disease, and then administer to the subject a therapeutically effective amount of one or more proteins or a gene delivery vehicle or pharmaceutical composition comprising one or more genes selected from the group consisting of Transmembrane protein 41B (Stasimon), Chromosome 19 open reading frame 54 (Rashomon), Tetraspanin 31, Poly (ADP-ribose) polymerase family member 1, Histidyl-tRNA synthetase-like, Chloride channel 7, and Nucleolar protein 1.

The invention makes 24-site mutation on natural scorpion chlorine ion channel neurotoxin gene BmK CT according to the principle of Escherichia coli partial to codon, designs DNA sequence suitable to be expressed in Escherichia coli BL21 (DE3), adopts PCR technique to complete artificial synthesis of recombinant scorpion chlorine ion channel neurotoxin gene rBmK CTa. On this basis, it clones rBmK CTa into pEXSecI expression system to transfer in the BL21 (DE3), screens and obtains high-performance expressed bacterial strain, detects that the expressed product of the rBmK CTa accounts for 19.936% of the all-bacterium protein by SDS-PAGE electrophoresis, and by affinity chromatography, obtains purer protein with bioactivity, and can obtain 2.4mg protein from one liter culture liquor by purifying. The obtained modified recombinant scorpion chlorine ion channel neurotoxin has inhibition effect on neuroglia cell and can be used in preparing medicines curing diseases by inhibiting neuroglia cell and also be used in research on the space structure and pharmacological activity of scorpion neurotoxin.

The present invention relates to compounds which act as modulators of the chloride channel CLC-7, assays for the identification of such compounds and methods of preventing, diagnosing, and treating osteoporosis and related disease states using such compounds.

The invention relates to agents and methods for the diagnosis, prognosis and treatment of cancer. Specifically, the invention relates to the use of nucleic and amino acid sequences encoding transmembrane superfamily member 6 (TM4SF6), synaptophysin like protein (SYPL), stomatin like 2 (STOML2), Ras related GTP binding protein RAGA), nucleotide sensitive chloride channel 1A (CLNS1A), prion protein (p27-30) (PRNP), guanine nucleotide binding protein beta 2-like 1 (GNB2L1), guanine nucleotide binding protein 4 (GNG4), integral membrane protein 2B (ITM2B), integral membrane protein 1 (ITM1), transmembrane 9 superfamily member 2 (TM9SF2), opiate receptor-like 1 protein (OPRL1), low density lipoprotein receptor-related protein 4 (LRP4), human glomerular epithelial protein 1 (GLEPP1), toll-like receptor 3 (TLR3), and / or zona pellucida glycoprotein 3A (ZP3) for the diagnosis of both early and late stage non-steroid specific cancers, cancer prognosis, as well as screening for therapeutic agents that regulate the gene expression and / or biological activity of said proteins. This invention further relates to the biological technologies designed to inhibit the gene expression and / or biological activity of said proteins including using agents identified in screening assays described herein, vector delivery of antisense polynucleotide sequences, and antibody targeting of said proteins. In specific embodiments, the proteins are of human origin.

The present invention relates to methods for treating a disease in an animal, which disease is responsive to blocking of chloride channel by administering to a mammal in need thereof an effective amount of a compound defined herein (including those compounds set forth in Tables 1-3 or encompassed by formula I-III) or compositions thereof

The invention relates to methods of and treatments for using pharmaceutical compositions from a class of compounds that directly or indirectly selectively modulates GABAA chloride channel activity to treat alcohol and / or stimulant substance abuse. The present invention also relates to methods of, and protocols for, relieving symptoms associated with alcohol and / or stimulant substance abuse in a comprehensive treatment plan. More specifically, the present invention relates to the use of a selective chloride channel modulator, such as flumazenil, to treat alcohol and / or psychostimulant dependency, the withdrawal symptoms associated therewith, and the cravings associated therewith.

The invention provides non-radioactive methods to assay for functional chloride channels. The methods colorimetrically detect the amount of iodide conducted by a chloride channel. They can be easily adapted for high throughput assays or screenings.

A compound containing at least two aromatic rings covalently bonded together, with each aromatic ring containing at least one oxyacetamide-based side chain, the compound being capable of forming a chloride ion channel across a lipid bilayer, and transporting chloride ion across the lipid bilayer.

Disclosed are the novel glycolipoprotein gintonin isolated and identified from ginseng, a method for preparing the same, and uses thereof. Gintonin causes a transient increase in intracellular free Ca2+ level, which in turn activates endogenous Ca2+-activated chloride channel to elevate intracellular calcium levels. Therefore, the novel glycolipoproteins are useful in the therapy and prophylaxis of calcium deficiency-associated diseases as well as effectively inducing calcium-dependent physiological activities, including adaptogenic activity, immunostimulatory activity, aphrodisiac activity, neuroprotection and neuroactivation, angiogenesis, and antidiabetic activity.

Provided herein are self-assembling compounds that can form ion channels in lipid bilayers or cell membranes and ion-channel-forming compositions comprising the self-assembling compounds. Also provided are methods of making and using the ion channels formed from a plurality of molecules of the self-assembling compounds. Further, provided are methods of treating or preventing conditions and diseases that are related to the dysfunction of ion channels, including chloride channels.

The invention belongs to the technical field of tobacco gene engineering, and particularly relates to a tobacco chloride ion channel protein gene NtCLC-F and application thereof. The base sequence of the gene is as shown in SEQ ID NO.1. The tobacco chloride ion channel protein NtCLC-F is composed of 726 amino acid residues, wherein the 181st-500th amino acids are conserved chloride ion channel protein structural domains. The protein is related to the chloride ion content of plant leaves, and after the expression of the protein is reduced, the chloride ion content of the leaves is obviously increased. Preliminary research on a specific tobacco chloride ion channel NtCLC-F shows that the specific tobacco chloride ion channel NtCLC-F is highly related to the content of the tobacco chloride ions, and after the gene is silenced, the content of the tobacco chloride ions is obviously increased. Based on the characteristic, a certain application basis and reference can be provided for cultivation of a new tobacco variety with the chlorine ion content regulated and controlled.

The invention discloses an application of borneol in promoting a poorly differentiated nasopharyngeal cell to intake adriamycin through chloride channel mediation. With the human poorly differentiated nasopharyngeal cell (CNE-2Z) as a research object, the conclusions are explored from the angle of the chloride channel as follows: (1) the chloride channel can be an action target point of the borneol in promotion of adriamycin transportation across a CNE-2Z cytomembrane; (2) the borneol can activate a CNE-2Z cell chloride current, and the current has volume sensitivity; (3) the ClC-3 chloride channel plays an important role in promotion of adriamycin transportation across the CNE-2Z cytomembrane by the borneol; and (4) the anti-tumor effect of the adriamycin can be obviously improved by combination of the borneol and the adriamycin. The mechanism of the borneol in promotion of drug uptake through the cytomembrane is expected to be diluted from the angle of the chloride channel by discussing the relationship between the chloride channel and the drugs taken by the cell in different states; and a new theoretical basis is provided for the borneol in clinical application.

The invention discloses an application of fipronil and an insect growth regulator to an anti-ectoparasite animal compound external preparation. The anti-ectoparasite animal compound external preparation comprises the fipronil and the insect growth regulator, and the weight ratio of the fipronil to the insect growth regulator is (0.1-20):(0.01-15). The preparation method includes the step: uniformly mixing the fipronil, the insect growth regulator and acceptable accessories. The anti-ectoparasite animal compound external preparation can effectively block a gamma-aminobutyric acid regulation chloride ion channel of an insect central nervous system, interfere the insect central nervous system and induce over-excitation of insect nerves and muscles until death, unhatched or ametabolic larvae cannot emerge into adults and die, and more complete overall expelling and killing effects of the preparation are ensured. Moreover, the preparation can more rapidly enter bodies to generate effects, and efficacy can be rapidly achieved.

In one aspect, there is provided a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:2 or SEQ ID NO:3 or SEQ ID NO:4 or SEQ ID NO:10 or SEQ ID NO:11; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:5 or SEQ ID NO:6 or SEQ ID NO:7 or SEQ ID NO:12 or SEQ ID NO:13 or SEQ ID NO:14; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i); and wherein the expression or activity of the polynucleotide or the polypeptide is modulated as compared to a control plant and wherein the nitrate levels in the mutant, non-naturally occurring or transgenic plant containing the mutant, non-naturally occurring or transgenic plant cell are modulated as compared to the control plant containing the control plant cell.

This invention relates to novel β-keto-amide derivatives that are found to be potent modulators of ion channels, and in particular potassium channels and chloride channels, and, as such, are valuable candidates for the treatment of diseases or disorders as diverse as those which are responsive to the modulation of potassium channels.

The present invention provides, inter alia, methods and pharmaceutical compositions for preventing, treating, or ameliorating the effects of a disease characterized by altered smooth muscle contractility, such as e.g., asthma and chronic obstructive pulmonary disease.

A pesticidal composition comprises a synergistically effective amount of a chloride channel activator compound and a pesticide selected from N-(3-chloro-1-(pyridin-3-yl)-1H-pyrazol-4-yl)-N-ethyl-3-((3,3,3-trifluoropropyl)thio) propanamide (I), N-(3-chloro-1-(pyridine-3-yl)-1H-pyrazol-4-yl)-N-ethyl-3-((3,3,3-trifluoropropyl) sulfinyl)propanamide (II), or any agriculturally acceptable salt thereof. A method of controlling pests comprises applying the pesticidal composition near a population of pests. A method of protecting a plant from infestation and attack by pests comprises contacting the plant with the synergistic pesticidal composition.

The invention discloses an application of hesperetin, tangeretin and glycyrrhetinic acid in inhibiting chloride ion channels, wherein the chloride ion channels can be inhibited; the invention discloses the application in development of drugs for treatment of diarrhea, heart disease, lung disease, stomach disease, brain disease, mental disease, rhinitis, otology diseases and ocular diseases. The detection verification method has raw materials easy to obtain, and the detection method is simple.

The present invention relates to the composition and methods of using a combination of a serotonin3 (5-HT3) receptor antagonist, such as ondansetron, and a selective chloride channel modulator, such as flumazenil, for treatment of patients with drug addiction or dependence. Examples of the drugs include prescription drugs as well as illegal drugs, such as, but not limited to, alcohol, cocaine, nicotine, marijuana, benzodiazepines and opiates. Furthermore, this combination can be used for treatment of patients suffering from central nervous system (CNS) disorders such as, but not limited to, depression, emotional and mood disorders and loss of memory.

The invention relates to a ds RNA inhabiting expression of chloride channel gene of aphid and an application thereof. Particularly, the ds RNA inhabiting expression of chloride channel gene of aphid is a double-stranded RNA composed of nucleotide of sequences represented by SEQ ID No:1 and the nucleotide of reverse complementary sequences. The application is that the ds RNA is introduced into aphid so as to prevent and control aphid, reduce aphid survival rate or inhabit expression of chloride channel gene of aphid. The invention uses the method of in vitro ds RNA feeding, and adopts the RNA i technology to silence expression of chloride channel gene of sitobion avenae to produce lethal effect on sitobion avenae. According to real-time fluorescence quantitative PCR study on chloride channel gene of aphid after feeding, expression of chloride channel gene is remarkably inhabited. The invention shows that sequences of chloride channel gene of aphid can be applied to research on increasing wheat resistance to aphids through plant mediating RNA i technology.

The present invention provides methods for the modulation of vascular tone in a patient having compromised vascular tissue, which methods comprise the administration of a chloride channel blocking agent or a pharmaceutically acceptable salt thereof.

The invention discloses a method for measuring intracellular chlorine concentration or chloride channel gene CFTR expression of human endometrial stromal cells in a menstrual cycle so as to predict prognosis of endometrial deciduation related diseases of the human endometrial stromal cells. Intracellular chlorine concentration up-regulation and chlorine channel gene CFTR expression down-regulation in endometrial stromal cells in a menstrual cycle can hinder deciduation of the endometrial cells, and finally, repeated abortion / repeated implantation failure is caused. Restoration of CFTR expression and / or reduction of chloride ion concentration in endometrial stromal cells in a menstrual cycle can improve uterine decidualization and improve the outcome of recurrent abortion / recurrent implantation failure. The invention develops a new field for clinical prediction and treatment of recurrent abortion / recurrent implantation failure, and has a good application prospect.