31 results about "Bacterial degradation" patented technology

The invention discloses a method for screening electricity-producing functional bacteria capable of degrading chlorophenols, the screened mixed bacteria and applications thereof. The present invention screens out electricity-producing bacteria capable of degrading chlorophenols from existing marine hydrothermal deposits, uses the bacteria to treat refractory chlorophenol wastewater in a microbial fuel cell system, and generates electricity through the microbial fuel cell system, which can Wastewater resources and energy. The invention has the advantages of low operating cost, convenient operating conditions, etc., and can effectively screen out objective functional bacteria and use them for wastewater treatment. The invention is a treatment process that integrates the screening of electricity-producing bacteria that degrades chlorophenols, sewage treatment, energy, and resource recovery, and has broad application prospects.

The invention provides bacillus aryabhattai GH-9 and an application thereof. The bacillus aryabhattai GH-9 is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number being CGMCC No.14519 on August 10th, 2017. The bacillus aryabhattai GH-9 has the following advantages: the bacillus aryabhattai GH-9 can degrade high-ammonia nitrogen and total nitrogen, accumulation of nitrite nitrogen and nitrate nitrogen is avoided, and nitrification and denitrification are performed synchronously. Tests prove that ammonia nitrogen with concentration being 500 mg / L can be degraded by a biological bacterial degradation agent prepared from the bacillus aryabhattai GH-9, the ammonia nitrogen degradation efficiency reaches 77%, and the total nitrogen degradationefficiency can reach 61%.

Methodologies, formulations, and kits suitable for decontaminating environments containing bacterial spores by degrading the spores. Formulations contain papain and at least one germinant. Methods for killing bacterial spores include contacting the spores with the formulation for a duration sufficient to initiate germination of the bacterial spores. The spores are then killed with the papain in the formulation, or are rendered by the papain susceptible to being killed by a reagent.

The invention discloses a method for preparing lambda-carrageenan oligosaccharide by carrying out bacterial degradation on lambda-carrageenan degrading bacteria. The method is characterized by comprising the following steps of: firstly, cultivating the lambda-carrageenan degrading bacteria which can generate extracellular lambda-carrageenan degrading enzymes, subsequently directly adding the bacterium liquid into a reaction liquid which contains 0.5-2.0% by weight of the lambda-carrageenan according to a volume ratio of 1:(0.8-1.2), reacting for 1-5 days at 20-30 DEG C under 100-150rpm, centrifuging so as to obtain supernate-containing oligosaccharide, adsorbing the oligosaccharide by using an activated carbon column, desorbing by using 20-40(v)% ethanol, concentrating, freezing and drying so as to obtain the lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8. The method has the advantages of being high in preparation process, high in yield, high in stability, and applicable to industrial production, and lambda-carrageenan oligosaccharide of which the polymerization degree ranges from 2 to 8 can be rapidly produced in large scale, so that the method has large application prospects in production of lambda-carrageenan oligosaccharide.

A transgenic Arabidopsis thaliana for repairing PCBs-contaminated soil, the invention comprises: subcloning TfdB and BphC genes from the soil metagenome; co-expressing TfdB+BphC plants through the construction of plant expression vectors, and plant genetic transformation mediated by Agrobacterium , screening of transgenic positive plants, transgenic plants repairing PCB 28 contaminated soil and other steps; constructing a pathway similar to bacterial degradation of PCBs in Arabidopsis, improving the tolerance of Arabidopsis to PCBs, reducing the limitation of PCB28 on plant growth, and improving The effectiveness of phytoremediation of PCBs-contaminated soil, and the acquisition of new phytoremediation materials for PCBs-contaminated soil; it has a good application prospect in reducing the threat of pollutants to the ecosystem and improving the ecological safety of PCBs phytoremediation.

The invention provides a protease-producing polylactic acid (PLA) degrading bacterium. The present invention isolates and screens a protease-producing polylactic acid-degrading bacterium Pseudomonas from a soil where polylactic acid / polybutylene terephthalate adipate (PLA / PBAT) mulch film is laid in Weifang, Shandong, and It is named Pseudomonas sp.strain LXM88, and is preserved in China General Microorganism Culture Collection and Management Center with the preservation number (CGMCC 18058). The advantage of the invention is that the bacterium produces protease for degrading PLA, and the bacterium has good pH tolerance and good ability to degrade PLA. The pseudomonas and its protease can be used as enzyme preparations, biological bacteria agents and biological enhancers for environmental restoration of PLA and PLA-based biodegradable materials in the environment, and have good application value.

The invention relates to a domestication kit and domestication method of a strain for coal coking wastewater COD (chemical oxygen demand) degradation. The kit comprises an enrichment pipe, a domestication prefabricated pipe A and a domestication prefabricated pipe B. The domestication method comprises anoxic domestication and aerobic domestication. According to the kit, abundant coal coking wastewater samples are acquired in all country, the wastewater composition is analyzed, a domestication culture medium capable of simulating true sewage is prepared through experimentation, and the kit is formed on such basis. The domestication culture medium has higher adaptability; and after the strain domesticated by the domestication culture medium is added into the plurality of coal coking sewage samples, the COD degradation capacity is enhanced, and the sewage treatment starting time is greatly shortened. The kit can simulate the anoxic and aerobic biological processes, can be used for optimizing the coal coking sewage treatment microbial inoculant product, and can also be used for quickly domesticating sludge before operation of the sewage plant.

The invention belongs to the field of environmental microbes, and specifically discloses a Klebsiella sp. bacterium CGMCC13201, which can effectively degrade crude oil in soil contaminated by crude oil, such as oily sludge and oily mud sand. In addition, the invention also discloses a method for using the bacteria to degrade oil stains.

The invention discloses treatment device and method for degrading residual oil through an oil-philic bacterium. The treatment device for degrading the residual oil through the oil-philic bacterium comprises a floating plate, wherein the edge of the floating plate is provided with floating barrels; the floating plate is provided, through a supporting frame, with solar cell panels which are connected with a storage cell pack; the end surface of one side of the floating plate is provided with propellers; at least one side of another three side surfaces of the floating plate is provided with a plurality of oil liquid decomposition devices; a residual oil degradation device is arranged under the floating plate among the floating barrels; the oil residual degradation device and the oil liquid decomposition devices each mainly comprises an oil liquid convergence layer, an oil absorption layer and a degradation layer; the total thickness of the oil liquid convergence layers, oil absorption paper and the degradation layers of each of the residual oil degradation device and the oil liquid decomposition devices does not exceed 1cm; one side of the floating plate is provided with a rotating shaft on which a number of the oil absorption layer is wound; the other side of the floating plate is provided with a rotary drum paired with the rotating shaft; the rotary drum is connected with a second motor; the oil absorption paper on the rotating shaft is arranged on the rotary drum after passing through the lower part of the floating plate.

The invention discloses a preparation method for fermentation broth of 1,3-propylene glycol, a treating method for the fermentation broth of 1,3-propylene glycol, application of a bacterial degradation supernatant and a solid containing an organic acid potassium salt and an organic acid salt produced by the treating method to preparation of a water-soluble fertilizer, a preparation method for the water-soluble fertilizer, and the water-soluble fertilizer prepared by using the method, belonging to the field of fermentation of 1,3-propylene glycol. The fermentation broth of 1,3-propylene glycol is obtained through microbial fermentation of glycerin; a neutralizer is used for adjusting the pH value of the fermentation broth in the process of fermentation; and the neutralizer contains more than 50 wt% of potassium hydroxide. The treating method comprises a step of treating the fermentation broth of 1,3-propylene glycol. According to the invention, direct or indirect pollution of by-products to environment is eliminated and production cost for the fertilizer is reduced on the basis that microbial fermentation conditions for production of 1,3-propylene glycol are guaranteed.