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Method for improving anaerobic energy production efficiency of algae raw material through bacterial degradation biological pretreatment

A biological pretreatment and algae technology, which is applied in the field of biotechnology and renewable energy, can solve the problems of decreased anaerobic gas production rate, enzymes cannot be reused, and high processing costs, so as to achieve high raw material utilization rate, improve energy conversion efficiency, The effect of promoting hydrolysis and breaking the wall

Active Publication Date: 2013-05-01
TONGJI UNIV
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Problems solved by technology

The results showed that high-pressure extrusion, microwave heating, and high-temperature drying pretreatment could improve the degradation efficiency of algae, but the organic matter in the algae had a certain loss after the first two methods, which led to a decrease in the subsequent anaerobic gas production rate; After treatment, the organic matter in the algae had a greater loss, and the gas production was lower than that of the untreated algae.
Compared with the mechanical method and heat treatment method, the enzymatic hydrolysis method has the advantages of high efficiency and small loss; and when the algae is pretreated by the enzymatic hydrolysis method, the action of the enzyme is selective and will not destroy other components; but the enzyme cannot be reused , it will be invalid after one use, so the processing cost is higher

Method used

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  • Method for improving anaerobic energy production efficiency of algae raw material through bacterial degradation biological pretreatment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for improving the anaerobic production efficiency of algae raw materials through bacteriolysis biological pretreatment:

[0028] (1) Centrifuge and concentrate the liquid of Scenedesmus subspicatus for later use.

[0029] Add 500ml of nutrient salt solution to the concentrated algae liquid, insert the pre-cultivated cellulose-degrading bacteria Clostridium sp. seed liquid, and control the temperature at about 55°C for pretreatment; Clostridium sp. The volume ratio of the solution was 5%.

[0030] The nutrient salt solution formula is per liter including NH 4 Cl, 1g; K 2 HPO 4 , 2g; KH 2 PO 4 , 3.3g; CaCl 2 , 0.1g; MgCl 2 ·6H 2 O, 0.2g; yeast powder, 0.1g; FeSO 4 ·7H 2 O, 1 mg; MnCl 2 4H 2 O, 1 mg; ZnCl 2 , 1 mg; CuCl 2 2H 2 O, 0.2 mg; NaMoO 4 2H 2 O, 1 mg in water; pH=7.0.

[0031] (2) After 20 hours of incubation and incubation in the dark, the TOC in the solution increased by 284 mg / L, but the total amount of ethanol and volatile fatty acids...

Embodiment 2

[0033] A method for improving the anaerobic production efficiency of algae raw materials through bacteriolysis biological pretreatment, such as figure 1 Shown:

[0034] (1) Centrifuge and concentrate the algae liquid of Chlorella vulgaris for later use.

[0035](2) Add 500ml of nutrient salt solution to the concentrated algae solution, insert the pre-cultivated Clostridium sp. seed solution, and insert anaerobic granular sludge at the same time, and control the temperature at about 55°C for anaerobic fermentation.

[0036] The formula of nutrient salt solution is as follows: NH 4 Cl, 0.5g; K 2 HPO 4 , 1g; KH 2 PO 4 , 1.7g; CaCl 2 , 0.05g; MgCl 2 ·6H 2 O, 0.1g; yeast powder, 0.05g; FeSO 4 ·7H 2 O, 0.5 mg; MnCl 2 4H 2 O, 0.5 mg; ZnCl 2 , 0.5mg; CuCl 2 2H 2 O, 0.1 mg; NaMoO 4 2H 2 O, 0.5mg; water, 500ml; pH=7.0.

[0037] The anaerobic granular sludge is taken from the anaerobic internal circulation reactor, which contains highly active methanogenic bacteria, an...

Embodiment 3

[0039] A method for improving the anaerobic production efficiency of algae raw materials through bacteriolysis biological pretreatment, such as figure 2 Shown:

[0040] (1) Centrifuge and concentrate the algae liquid of Chlorella vulgaris for later use.

[0041] Add 500ml of nutrient salt solution to the concentrated algae solution, insert the pre-cultivated Clostridium sp. seed solution, and control the temperature at about 55°C for pretreatment.

[0042] (2) After culturing for 4 days, insert anaerobic granular sludge and carry out anaerobic fermentation at about 55°C.

[0043] The formula of nutrient salt solution is as follows: NH 4 Cl, 0.5g; K 2 HPO 4 , 1g; KH 2 PO 4 , 1.7g; CaCl 2 , 0.05g; MgCl 2 ·6H 2 O, 0.1g; yeast powder, 0.05g; FeSO 4 ·7H 2 O, 0.5 mg; MnCl 2 4H 2 O, 0.5 mg; ZnCl 2 , 0.5mg; CuCl 2 2H 2 O, 0.1 mg; NaMoO 4 2H 2 O, 0.5 mg; water, 500 ml; pH 7.0.

[0044] (3) After the synchronous hydrogen production culture by bacterial hydrolysis, an...

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Abstract

The invention belongs to the field of biotechnology and renewable energy sources, and relates to a method for improving the anaerobic energy production efficiency of an algae raw material through bacterial degradation biological pretreatment. The method comprises the following steps: (1), the algae raw material is mixed with a nutritive salt solution to be inoculated to an anaerobic cellulose degrading bacteria seed solution which is cultivated in advance; (2), the seed solution is cultivated under the conditions of thermal insulation and opaque background, the bacterial degradation pretreatment is performed, hydrogen is synchronously produced, hydrolysis of cell walls is promoted, and hydrogen, hydrolysis product ethanol and volatile organic acid are obtained; and (3), after the bacterial degradation synchronous hydrogen production cultivation is finished, anaerobic inoculated sludge containing methanogens is inoculated in fermentation liquor, and accordingly hydrolysis product ethanol, volatile organic acid and algae cell residues after the bacterial degradation suffer anaerobic fermentation for methanogenesis. According to the invention, the algae biomass raw material is subjected to pretreatment through bacterial degradation so as to promote hydrolysis wall breaking of algae cells; and moreover, higher yield of hydrogen is simultaneously obtained in the process of anaerobic fermentation methanogenesis, thereby improving the energy conversion efficiency of the algae biomass raw material.

Description

technical field [0001] The invention belongs to the fields of biotechnology and renewable energy, and relates to a method for improving the anaerobic production efficiency of algae raw materials through bacteriolysis biological pretreatment. Background technique [0002] In the context of energy and environmental crisis, it is imperative to seek clean, safe and sustainable energy. The use of renewable energy can reduce dependence on petroleum and reduce environmental pollution. In this context, the development of biomass renewable energy has attracted more and more attention from the society, and has become an important choice for the diversification of energy sources and the national energy strategy to resist risks. Algal raw material is one of the important biomass resources. Algae is a group of autotrophic plants with various species and extremely wide distribution in lower plants. Compared with other biomass such as lignocellulosic materials (wood), algae have the cha...

Claims

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Application Information

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IPC IPC(8): C12P5/02C12P3/00C12P7/06C12P7/40C12R1/145
CPCY02E50/17Y02E50/10Y02E50/30Y02P20/59
Inventor 吕凡季佳琪何品晶毛斌邵立明吴铎柴丽娜
Owner TONGJI UNIV
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