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Bacteria domestication kit and domestication method for cod degradation of coal coking wastewater

A coal coking and kit technology, which is applied to the bacterial species domestication kit for COD degradation of coal coking wastewater and the field of domestication, can solve the problems of refractory organic matter, long startup time, long time, etc., and achieves the improvement of COD degradation ability and adaptation. The effect of ability, wide applicability

Active Publication Date: 2020-02-28
TIANJIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the treatment of coal coking wastewater, the effect of adding sludge and bacterial agents is restricted by the following problems: 1. Coal coking wastewater contains a variety of substances that are toxic to microorganisms, and the sludge added to sewage or Some microorganisms in the strains are easily inhibited by toxic substances in the wastewater. To solve this problem, it is often necessary to domesticate the sludge or strains for a long time before adding them; 2. There are a large amount of refractory organic matter in coal coking wastewater, These substances are difficult to be utilized and degraded by microorganisms. To solve this problem usually requires the rational design of the process and the domestication of sludge or bacterial agents; 3. Compared with ordinary sewage, the start-up time of coal coking wastewater treatment facilities is longer and requires longer domestication

Method used

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  • Bacteria domestication kit and domestication method for cod degradation of coal coking wastewater
  • Bacteria domestication kit and domestication method for cod degradation of coal coking wastewater
  • Bacteria domestication kit and domestication method for cod degradation of coal coking wastewater

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Experimental program
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Effect test

Embodiment 1

[0049] The kit used in embodiment 1 and the preparation method are as follows:

[0050] 1. Enrichment tube:

[0051] Preparation of enrichment tubes:

[0052] 1. Take 2g of beef extract, 2g of peptone, 780mg of potassium dihydrogen phosphate, and 1.25g of disodium hydrogen phosphate, and dissolve them in 1L of water. Use 5% sodium carbonate and 5% HCl to adjust the pH of the above medium to 7.00. Add 20ml of culture medium to each 50ml screw tube; after dispensing, unscrew the cap of the screw tube and sterilize at 115°C for 25 minutes. After the sterilization is completed and cooled to room temperature, take it out from the sterilizer and tighten it Tube cap, so far the preparation of the basic enrichment tube is completed;

[0053] 2. Take 22 mg of phenol, 7 mg of naphthol, 7 mg of 2-methylphenol, 7 mg of 3-methylphenol, and 2 mg of quinoline, dissolve them in 100 ml of sterile water, and filter them with a 0.22 μm sterile filter membrane under sterile conditions Steriliz...

Embodiment 2

[0090] The kit used in embodiment 2 and the preparation method are as follows:

[0091] Rich tube:

[0092] 1. Preparation of the enrichment tube:

[0093] 1. Take 1 g of beef extract, 1 g of peptone, 640 mg of potassium dihydrogen phosphate, and 0.70 g of disodium hydrogen phosphate, dissolve them in 1 L of water, adjust the pH of the above culture medium to 7.50 with 5% sodium carbonate and 5% HCl, and divide into Add 20ml of medium to each 50ml screw tube; after dispensing, unscrew the cap of the screw tube, sterilize at 115°C for 30min, after the sterilization is completed and cooled to room temperature, take it out from the sterilizer and tighten it Tube cap, so far the preparation of the basic enrichment tube is completed;

[0094] 2. Take 18mg of phenol, 3mg of naphthol, 3mg of 2-methylphenol, 3mg of 3-methylphenol, and 1mg of quinoline, dissolve it in 100ml sterile water, and filter it with a 0.22μm sterile filter membrane under sterile conditions Sterilization: Aft...

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Abstract

The invention relates to a domestication kit and domestication method of a strain for coal coking wastewater COD (chemical oxygen demand) degradation. The kit comprises an enrichment pipe, a domestication prefabricated pipe A and a domestication prefabricated pipe B. The domestication method comprises anoxic domestication and aerobic domestication. According to the kit, abundant coal coking wastewater samples are acquired in all country, the wastewater composition is analyzed, a domestication culture medium capable of simulating true sewage is prepared through experimentation, and the kit is formed on such basis. The domestication culture medium has higher adaptability; and after the strain domesticated by the domestication culture medium is added into the plurality of coal coking sewage samples, the COD degradation capacity is enhanced, and the sewage treatment starting time is greatly shortened. The kit can simulate the anoxic and aerobic biological processes, can be used for optimizing the coal coking sewage treatment microbial inoculant product, and can also be used for quickly domesticating sludge before operation of the sewage plant.

Description

Technical field: [0001] The invention belongs to a strain domestication kit and a domestication method, in particular to a strain domestication kit and a domestication method for degrading coal coking wastewater COD. Background technique: [0002] Coal coking is also called coal high temperature dry distillation. It is a coal conversion process that uses coal as raw material and heats it to about 950°C under air-isolated conditions to produce coke through high-temperature dry distillation, obtain gas, coal tar and recover other chemical products at the same time. [0003] Water pollution caused by the coal coking industry is quite serious. my country has a large coal reserve and a large amount of coal coking wastewater. The composition of the wastewater is quite complex. The discharged wastewater contains a large amount of organic pollutants that are difficult to degrade. According to relevant statistics, coal coking wastewater contains more than 300 kinds of pollutants. Wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
Inventor 宋文军于春花李博智李霏
Owner TIANJIN UNIV OF COMMERCE
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