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Screening system for microbial strains with different keratin degradation capabilities

A technology of keratin degradation and microbial strains, which is applied in the field of screening system of microbial strains with different keratin degradation capabilities, can solve problems such as inconclusiveness, and achieve the effect of simple steps, short cycle and strong repeatability

Inactive Publication Date: 2018-02-23
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Microbial degradation of hard keratin such as feathers is a complex process. Current research shows that the process of microbial degradation of feathers is not only the function of keratinase, but also requires the direct participation of other molecules and even microorganisms. But how is this process led and completed by microorganisms? has been inconclusive

Method used

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  • Screening system for microbial strains with different keratin degradation capabilities
  • Screening system for microbial strains with different keratin degradation capabilities
  • Screening system for microbial strains with different keratin degradation capabilities

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1 Implementation materials

[0025] Microbial species: Stenotrophomonas maltophilia DHHJ, a bacterium that degrades feathers;

[0026] Feather powder: Wash the chicken feathers with detergent, dry them at 80°C for 48 hours, shred them and crush them with a pulverizer.

Embodiment approach

[0028] 2.1 Choose the right feather powder plate

[0029] Prepare different feather meal concentrations, the feather meal plates of agar concentrations, as shown in Table 1:

[0030] Table 1 Composition of the initial feather meal flat plate (100mL)

[0031]

[0032] After high temperature and high pressure sterilization, the feather meal is unevenly distributed in the medium and is easy to agglomerate. After being cultured by dropping the bacterial solution, the contrast between the bacteria and the plate is low, and it is difficult to observe the growth of the bacteria. In order to solve the above problems, the feather meal plate was improved. recipes such as figure 2 shown.

[0033] Feather meal flat plate composition (100mL) after the improvement of table 2

[0034]

[0035] Feather powder is evenly distributed in the culture medium without agglomeration. After being cultured by dropping the bacterial solution, the contrast between the bacteria and the plate is h...

Embodiment 2

[0046] (1) The preparation of feather meal flat plate: get the feather meal 1g that embodiment 1 obtains, glucose 1g, potassium chloride 0.04g, sodium chloride 0.03g, Tween 80 0.4g, agar 2g, disodium hydrogen phosphate 0.4g, Potassium dihydrogen phosphate 0.03g, pH = 7.5 to prepare a feather meal flat plate.

[0047] (2) Cultivation of the second-generation bacterial liquid of the transformant: Inoculate Stenotrophomonas maltophilia DHHJ on a feather meal plate, pick a single colony of the microorganism to be identified, inoculate it in a test tube containing 5mL of beef extract peptone medium, and place it at 35°C, 160rpm Cultivate for 12 to 16 hours to obtain the transformant bacterial liquid, inoculate 500 μL of the bacterial liquid from the above-mentioned test tube into an Erlenmeyer flask containing 50 mL of beef extract peptone medium according to the inoculation ratio of 1%, and culture it at 35°C and 160 rpm until OD 600 =0.6, the second-generation bacterial liquid of...

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Abstract

The invention relates to a screening system for microbial strains with different keratin degradation capabilities. The screening system comprises feather meal plates and transformant secondary-generation bacteria solution. The screening system has the advantages of simple steps, short cycle and high repeatability. Besides, mutants with weakened keratin degradation capabilities or without the keratin degradation capability can be quickly and accurately screened by the screening system, and accordingly foundations can be laid for carrying out deep research on feather degradation mechanisms of bacteria, finding genes related to keratin degradation and constructing new-generation efficient strains.

Description

technical field [0001] The invention belongs to the technical field of biochemistry and molecular biology, and particularly relates to a screening system for microbial strains with different keratin degradation abilities. Background technique [0002] my country's feather resources are extremely rich, but they are not fully utilized, and some even cause environmental pollution. According to measurements, the crude protein content of feathers is more than 80% (Fu Hongmei. Research on the technology of producing soluble protein by hydrolysis of feather powder [J]. Journal of Sichuan Union University (Engineering Science Edition), 1997, 6 (1): 65-71 .), the amino acid content is above 70%, and it also contains macro elements, trace elements, vitamins and some unknown growth factors. Keratin is insoluble in water and anti-decomposition. It is a hard protein with strong resistance. Its structure forms a highly cross-linked three-dimensional stable structure through intermolecula...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 曹张军郝亚楠张卫
Owner DONGHUA UNIV
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