37 results about "Aspergillus parasiticus" patented technology

The invention belongs to the technical field of microbial fermentation, and discloses a method for separating L-malic acid, which comprises the following steps: step 1) preparing Aspergillus oryzae seed liquid, step 2) preparing Aspergillus parasiticus seed liquid, step 3) fermenting, Step 4) Centrifugation, filtration and ion exchange. The invention has the advantages of high product yield and purity for separating L-malic acid from the fermentation liquid, simple operation process, low production cost and large-scale production.

The invention provides a method for producing malic acid by using fermentation of wheat bran hydrolysate. The method comprises the following steps: preparing wheat bran hydrolysate by an acid hydrolysis method, and then carrying out detoxification treatment on a fermentation inhibitor contained in the hydrolysis liquid glucose by using activated carbon; and selecting aspergillus parasiticus CICC40365 with good adaptability, and matching with the optimized fermentation nutrients to mix and ferment, so as to obtain malic acid fermentation liquor. The method has advantages of being high in wheat bran hydrolysis rate, fast in reaction speed, simple in process and low in cost, the hydrolysis liquid glucose is low in loss in the fermentation process by good detoxification treatment, and the adopted aspergillus has good adaptability, and is high in conversion rate of the malic acid. In addition, the method is free of poison and harm in the production process, environment-friendly and energy-saving, applicable to industrial large-scale production, and significant in economic benefits may be achieved, energy crisis in China and in the world can be effectively relieved.

The invention relates to a serratia plymithica synthetic culture medium and a method for preparing fermentation liquid of the serratia plymithica synthetic culture medium. The formula (gram / liter) of the culture medium comprises the following components of 2 to 10 of colloidalchitin, 3 to 10 of beef extract, 5 to 15 of peptone, 6 to 10 of sodium chloride, 1 to 5 of ammonium sulfate, 1 to 3 of sodium citrate, 1 to 5 of dipotassium phosphate, 0.5 to 3 of magnesium sulfate and 0.01 to 0.05 of ferrous sulfate. The serratia plymithica high-yield fermentation liquid for producing a large quantity of active substances and chitinase which are used for suppressing aflatoxin can be obtained by continuously culturing on a table concentrator at the temperature within 28 and 35 DEG C and the speed of 140 r / min for 4 to 6 days according to the inoculation quantity within 1 to 10 percent. The activity of the chitinase in fermentation supernate is 2.04 to 5.36U / ml; the rate of suppressing the growth of parasitic aspergillus silk is 80.13 to 89.56 percent; and the rate of suppressing the aflatoxin is 90.22 to 98.31 percent. The culture medium is rational in component, and the preparation method is simple and reliable.

The invention belongs to the technical field of fermentation engineering, and specifically relates to an AFG2 toxin production fermentation method of aspergillus parasiticus. The method comprises: a,performing reactivation propagation to aspergillus parasiticus spores by adopting an enrichment medium; and b, performing solid fermentation culture through adoption of a solid fermentation medium, wherein each of the enrichment medium and the solid fermentation medium contains 3-5% of yeast extract. The method is used for solving the problem that grain naturally infected with mould is low in AFG2concentration and nonuniform in AFG2 and the culture time is long.

The invention discloses a primer sequence used to identify Aspergillus parasiticus and Aspergillus flavus; the primer sequence comprises two sets of primers; the forward primer in the first set of primers is provided with the base sequence of SEQ ID NO:1 in a sequence table, and the reverse primer of the first set of primers is provided with the base sequence of SEQ ID NO:2 in the sequence table;the forward primer in the second set of primers is provided with the base sequence of SEQ ID NO:3 in the sequence table, and the reverse primer of the second set of primers is provided with the base sequence of SEQ ID NO:4 in the sequence table. The primer sequence has high specificity, identities Aspergillus parasiticus and Aspergillus flavus through PCR amplification with high speed and reliability, thereby laying theoretical foundation for researching the soil polluted by aflatoxin or the distribution and dynamic changes of two types of fungi in crops and providing scientific bases for thecontrol of aflatoxin.

The invention provides a strain of Aspergillus parasiticus, which is Aspergillus parasiticus RWSF001‑02, which is preserved in the "Guangdong Microbial Culture Collection Center" with a preservation number of GDMCC NO: 60080. The Aspergillus parasitica is selected from aphids that died naturally due to fungal parasitism. The Aspergillus parasitica and the spore powder containing the Aspergillus parasitica spores can be used to prepare insecticides, especially for aphids with a high control rate, and the strain has good production properties and rapid growth. After 5 days, the sporulation amount can reach 8×108 / g.

The invention relates to the field of bioengineering, and relates to a preparation method and application of an inoculum composition for strengthening watercress post-fermentation. The inoculant composition includes yeast inoculum, aspergillus oryzae inoculum and Gesporum coelomyces inoculum, which are prepared separately The three bacterial agents are then mixed with the post-fermented watercress in proportion for the post-fermentation of the watercress. The invention provides a preparation method of the bacterial agent composition, the overall process is suitable for industrial continuous production, comprehensively utilizes the by-product of Pixian watercress production (pepper bellflower), the production cycle can be saved for 6 months, the amino nitrogen is increased by 20%, and the volatility is high. Fragrance components increased by more than 3 times (total ester, total acid and total aldehyde content), in aflatoxin B 1 The peak produced (fermentation 30-60 days) was inserted into the yeast agent, which strengthened the process of ester production and aroma production, competitively inhibited the metabolism of toxin-producing Aspergillus flavus and some Aspergillus parasitica, and reduced the content of aflatoxin B1. Aspergillus toxin B 1 Less than 0.5ppm improves food safety.

The invention relates to a strain of bacillus amyloliquefaciens with high fungal inhibitory activity and application thereof, belonging to the field of biotechnology application. The invention provides a new bacterial strain; a Bacillus amyloliquefaciens, the preserved strain name is Bacillus amyloliquefaciens F1, its preservation number is CGMCCNo.10942, the preservation date is June 1, 2015, and the preservation unit is China Microbial Strain Collection Committee General Microbiology Center (CGMCC). The Bacillus amyloliquefaciens F1 with the preservation number of CGMCC No. 10942 of the present invention can produce antifungal protein, and can effectively inhibit the growth and toxin production of fungi such as Aspergillus niger, Aspergillus white, Aspergillus parasitica and Aspergillus flavus. The antibacterial protein produced by the bacterial strain of the invention can resist the infection of the mold on the black pepper, weaken the growth ability of the mold hyphae, protect the active ingredients of the black pepper, and reduce the loss and deterioration of the quality of the black pepper. The bacterial strain and its metabolites of the invention can be developed into a residue-free, safe and efficient food protection agent, which prolongs the shelf life of food and improves food safety.

The invention discloses a method for preparing epipodophyllotoxin diglucoside and a special bacterial strain thereof. The strain is Aspergillus parasiticus TEQB, and its preservation number is CGMCC No.3116. The bacterial strain of the present invention can be used to prepare epipodophyllotoxin diglucoside. The method for preparing epipodophyllotoxin diglucoside of the present invention has the advantages of simple operation, low cost, easy operation and short production cycle. The method of the present invention provides a new way for preparing epipodophyllotoxin diglucoside, avoids the limitations of other various preparation methods in the prior art, has strong operability, is suitable for industrialized large-scale production, and simultaneously It is of great significance to protect the biodiversity of plants.