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Antifungal peptide and application thereof to inhibition of generation of aflatoxin

A kind of technology of aflatoxin and antifungal peptide, which is applied in the field of polypeptides, can solve the problems that it is not suitable for large-scale production and application, and the inhibitory effect of aflatoxin is limited, so as to inhibit the growth and production of aflatoxin, and the inhibitory effect is good, Easy to prepare

Active Publication Date: 2014-09-24
BEIJING YUANSHENGYUAN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides an antifungal peptide and its application in inhibiting the production of aflatoxin, which is used to solve technical defects such as limited inhibitory effect on aflatoxin in the prior art, unsuitable for large-scale production and application, etc.

Method used

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  • Antifungal peptide and application thereof to inhibition of generation of aflatoxin
  • Antifungal peptide and application thereof to inhibition of generation of aflatoxin
  • Antifungal peptide and application thereof to inhibition of generation of aflatoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of antifungal peptides:

[0025] The antifungal peptide whose amino acid sequence is Ala-Asp-Leu-Pro-Val-Lys-Ser-Gly-Asp-Thr-Pro-Glu was synthesized by ABI433A automatic peptide synthesizer, and desalted by HPLC reversed-phase column chromatography, and used The molecular weight of the antifungal peptide determined by Tricine-SDS-PAGE was 1380Da.

Embodiment 2

[0027] The antifungal peptides prepared in Example 1 were dissolved in sterile water to prepare antifungal peptide solutions with mass percent concentrations of 0.01% and 0.03% respectively, and antibacterial tests were carried out.

[0028] The cultured Aspergillus flavus and Aspergillus parasitica were respectively prepared so that the spore concentration was 10 5 CFU mL -1 The left and right spore suspensions are respectively added to the plates with sterilized solid medium (such as PDA medium), and about 20mL is added to each plate, then a sterilized Oxford cup is placed in each plate, and the Oxford cup of each plate is added. 100 μL of the above-mentioned antifungal peptide solutions with concentrations of 0.01% and 0.03% were respectively added into the cups as test examples 2-1 and 2-2, and sterile water was used as control example 2-1.

[0029] After each plate was placed in a constant temperature incubator at 28°C for 72 hours, the diameter of the inhibition zone wa...

Embodiment 3

[0035] The antifungal peptide prepared in Example 1 was dissolved in sterile water to prepare antifungal peptide solutions with mass percentage concentrations of 0.02% and 0.05% respectively.

[0036] Take common commercially available corn kernels (moisture content is about 15%), detect its aflatoxin content and divide it into four batches, and spray the above-mentioned antifungal peptide solutions with concentrations of 0.02% and 0.04% and sterile water evenly in them On the three batches of corn kernels, as test example 3-1, 3-2 and comparative example 3-1, and control the water content of three batches of corn kernels after spraying to be about 22%; Another batch of corn kernels without any treatment is used as Comparative example 3-2.

[0037] After the above four batches of corn kernels were packaged by a packaging machine, they were stored in a storage room at room temperature, and samples were taken on the 10th day, 30th day, 60th day and 90th day after storage, and th...

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Abstract

The invention provides an antifungal peptide and an application thereof to inhibition of generation of aflatoxin. The antifungal peptide has the amino acid sequence as follows: Ala-Asp-Leu-Pro-Val-Lys-Ser-Gly-Asp-Thr-Pro-Glu. The antifungal peptide provided by the invention is capable of effectively inhibiting the growth of aspergillus flavus and aspergillus parasiticus and the generation of aflatoxin, favorable and durable in inhibiting effect and suitable for large-scale production and application.

Description

technical field [0001] The invention relates to a polypeptide, in particular to an antifungal peptide and its application in inhibiting the production of aflatoxin. Background technique [0002] Aflatoxins are a class of compounds with similar chemical structures, mainly produced by Aspergillus flavus and A. parasiticus, B1, B2, G1, G2, M1, M2 have been isolated and identified There are more than a dozen kinds of aflatoxins, the basic structures of which are dihydrofuran ring and coumarin. Aflatoxin is a highly toxic and highly toxic substance. Its harmfulness mainly lies in its destructive effect on the liver tissue of humans and animals. In severe cases, it can cause liver cancer or even death. [0003] Aspergillus flavus and Aspergillus parasitica exist widely in the air and soil, and after they infect peanuts, corn and other food crops, they will produce aflatoxins during the storage of these crops. Aflatoxin B1 is the most common in naturally contaminated food, and it...

Claims

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Application Information

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IPC IPC(8): C07K7/08A23L3/3544
Inventor 于赞祝传斌牛旼
Owner BEIJING YUANSHENGYUAN SCI & TECH DEV
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