Toxigenic medium of AFG2, and AFG2 toxigenic fermentation method of Aspergillus parasiticus

A technology of toxin-producing medium and Aspergillus parasiticus, applied in the field of fermentation engineering, can solve the problems of long time consumption, unevenness, and low AFG2 concentration, and achieve the effects of shortened culture time, high spore vigor, and low production cost

Active Publication Date: 2015-04-22
南京金桔饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides an AFG2 toxin-producing medium, which is used to solve the problems of low, uneven and time-consuming AFG2 concentrations in grains naturally infected with molds

Method used

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  • Toxigenic medium of AFG2, and AFG2 toxigenic fermentation method of Aspergillus parasiticus
  • Toxigenic medium of AFG2, and AFG2 toxigenic fermentation method of Aspergillus parasiticus
  • Toxigenic medium of AFG2, and AFG2 toxigenic fermentation method of Aspergillus parasiticus

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Experimental program
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Effect test

Embodiment 1-4

[0018] The preparation of embodiment 1-4 enrichment culture medium

[0019] A bacterium enrichment medium for Aspergillus parasiticus strains, after mixing the components shown in Table 1, adjust the pH value of the bacterium enrichment medium to 5.5-7.0 with hydrochloric acid and sodium hydroxide solution.

[0020] Table 1

[0021]

Embodiment 5-8

[0022] The preparation of embodiment 5-8 compound mineral salt

[0023] Take sodium nitrate, magnesium sulfate, potassium chloride, dipotassium hydrogen phosphate, zinc sulfate, manganese sulfate and ferrous sulfate pentahydrate according to the ratio in Table 2, mix the above mineral salts evenly, and pulverize them through an 80-mesh sieve , mixed again for later use, to obtain the compound mineral salts described in the enrichment medium and solid fermentation medium.

[0024] Table 2

[0025]

[0026] Now take the compound mineral salt formula of embodiment 6 and the bacterium enrichment medium formula of embodiment 2 as examples, prepare 100g compound mineral salt and 1L bacterium enrichment medium.

[0027] Composite mineral salt (100g): take by weighing 44.0g sodium nitrate, 14.0g magnesium sulfate, 12.0g potassium chloride, 20.0g dipotassium hydrogen phosphate, 5.7g zinc sulfate, 4.0g manganese sulfate, 0.3g pentahydrate with analytical balance respectively Mix fe...

Embodiment 9-12

[0032] The preparation of embodiment 9-12 Aspergillus parasiticus solid fermentation medium

[0033] According to the formula in Table 3, prepare the required solid fermentation medium.

[0034] table 3

[0035]

[0036] Raw material preparation: crush the indica rice and cottonseed meal, and it is required to pass through a 20-mesh standard sampling sieve, but not through a 40-mesh standard sampling sieve, that is, the particle size is between 20-40 mesh.

[0037] Now take the compound mineral salt formula of embodiment 6 and the component of embodiment 10 as example, prepare 100g compound mineral salt and 1L bacteria enrichment medium.

[0038] Composite mineral salt (100g): take by weighing 44.0g sodium nitrate, 14.0g magnesium sulfate, 12.0g potassium chloride, 20.0g dipotassium hydrogen phosphate, 5.7g zinc sulfate, 4.0g manganese sulfate, 0.3g pentahydrate with analytical balance respectively Mix ferrous sulfate in a beaker evenly, then put all the mixed mineral salts...

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Abstract

The invention relates to a toxigenic medium of AFG2, and an AFG2 toxigenic fermentation method of Aspergillus parasiticus. The medium contains 3-5wt% of yeast extract, and a fermentation medium comprises an enrichment medium and a solid fermentation medium. The toxigenic medium of AFG2 solves the problems of low concentration of the AFG2 in present cereal naturally infecting molds, non-uniformity and long time.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to an AFG2 toxin-producing medium and an AFG2 toxin-producing fermentation method of Aspergillus parasiticus. Background technique [0002] Aflatoxins (AFs) are secondary metabolites produced by some toxin-producing fungi such as Aspergillus parasiticus, Aspergillus flavus and Aspergillus jifeng, which have triple effects of carcinogenicity, teratogenicity and mutagenesis. According to literature reports, more than 20 types of AFs have been identified, the most common of which are aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG2), and aflatoxin G2. (AFG2). AFs can contaminate corn, wheat, soybean, cottonseed and other grain and oil-bearing agricultural products, and can also remain in some agricultural by-products such as DDGS, wheat bran, soybean meal and cottonseed meal. When AFs-contaminated agricultural products are used as human food, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12N1/14C12R1/66
Inventor 熊江林侯永清丁斌鹰刘玉兰邱银生吴灵英
Owner 南京金桔饲料有限公司
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