A preparation method and application of a composition for strengthening watercress post-fermentation bacteria
A technology for fermentation inoculum and watercress, which is applied in the field of preparation of fermented inoculum composition after strengthening watercress, can solve problems such as hidden dangers of food safety, production of musty odor, etc., and achieve the effects of reducing content and improving food safety.
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Embodiment 1
[0017] A preparation method for strengthening watercress post-fermentation inoculum composition, characterized in that, said inoculum composition includes saccharomyces, aspergillus oryzae inoculum and Gesporum coelomyces agent, and the preparation method is as follows:
[0018] Preparation of yeast agent: Pichia anomaly and Torulopsis globosa in the logarithmic growth phase were cultured at 5 ° Be' wort at 30 ° C for 3 days at 3-5% (v / v) inoculum, and then in the fermentation broth Add 6% of porous starch, 3% of sucrose and 1% of glycerin, freeze and vacuum dry at a temperature of -40°C to -45°C, and freeze-dry for 24-48 hours to obtain a yeast agent;
[0019] The preparation of aspergillus oryzae inoculant: the aspergillus oryzae in logarithmic growth phase is cultivated 2-5 days at 6~7 ° of Bé rice koji juice, 28-30 ℃ by 3-5% (v / v) inoculum, makes rice koji When the juice appears slightly yellow, after the cultivation is completed, add porous starch, 8% sucrose and 1% glyce...
Embodiment 2
[0023] An application method for strengthening the post-fermentation microbial composition of watercress in watercress post-fermentation. During the post-fermentation of watercress, when the fermentation reaches 15-30 days, the Aspergillus oryzae inoculum is added according to the mass ratio of 0.2:10000 and mixed evenly for fermentation. When the fermentation reaches 30-60 days, add the yeast agent according to the mass ratio of 0.1:10000 and mix evenly. When the fermentation reaches more than 90 days, add the fermented Gesperia columella according to the mass ratio of 0.05:10000.
Embodiment 3
[0025] Inoculum composition of the present invention is effective against aflatoxin B in watercress post-fermentation 1 Impact.
[0026] (1) Sample collection
[0027] Add Pichia anomala CICC 1332, Pleospora sp. BNCC179839, Torulopsis globosa BNCC176445 and Aspergillus oryzae ATCC 41416 alone or in combination, totaling 40 batches Post-fermentation of Pixian Douban samples, 5 parallel experiments were done for each addition. (See table below for results)
[0028] (2) Instruments and reagents
[0029] Instruments: 90mm mortar (Tangshan Kaiping Shengxing Chemical Porcelain Factory); constant temperature culture oscillator (Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.); vortex oscillator (Germany IRM company); microplate reader (US Bio-Rad company) ; Constant temperature water bath (Jintan Fuhua Instrument Equipment Company).
[0030] Reagents: methanol, n-hexane, chloroform (all of analytical grade, Chengdu Kelong Chemical Reagent Factory), aflatoxin B1 (...
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