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Serratia plymithica synthetic culture medium and method for preparing fermentation liquid of serratia plymithica synthetic culture medium

A Serratia culture medium technology, applied in the field of Serratia fermentation synthesis medium and fermentation, to achieve the effect of reasonable collocation and rich nutrition

Inactive Publication Date: 2015-05-20
HARBIN INST OF TECH AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports on the culture medium and fermentation conditions of Serratia producing chitinase at home and abroad, but none have been used for the high production of chitinase in Serratia. At the same time, the fermentation product can effectively prevent and control aflatoxin in peanuts. Synthetic media and fermentation methods reported

Method used

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Examples

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preparation example Construction

[0014] A preparation method of Serratia fermented liquid, it comprises the steps:

[0015] (1) Preparation of culture medium for chitinase-producing Serratia strains, the medium formula (g / L) is: colloidal chitin 2-10, beef extract 3-10, peptone 5-15, sodium chloride 6~10, ammonium sulfate 1~5, sodium citrate 1~3, K 2 HPO 4 1~5, MgSO 4 0.5~3, FeSO 4 ·7H 2 O 0.01~0.05;

[0016] (2) Seed culture: The medium is glucose 20 g / L, yeast powder 5 g / L, initial pH 6.8-7.2; culture conditions: shaker 140 r / min at 30°C for 12 hours;

[0017] (3) Liquid fermentation culture: transfer the liquid seeds to the high-yield medium according to the inoculum size of 1-10%, and continuously culture in a shaker at 28-35°C at 140 r / min for 4-6 days to obtain chitin-containing Fermentation broth of Serratia sp. with protease and aflatoxin-inhibiting active substances.

Embodiment 1

[0019] Bacterial species: Serratia sp., a new strain of Serratia sp. that is highly effective in inhibiting aflatoxin, was isolated from the soil of peanut pods in Huaian City, Jiangsu Province by the Harbin Institute of Technology (Weihai) Marine Science and Technology .

[0020] Serratia fermentation synthetic medium formula (g / L) is: colloidal chitin 4, beef extract 3, peptone 12, sodium chloride 6, ammonium sulfate 5, sodium citrate 1, K 2 HPO 4 5. MgSO 4 0.5, FeSO 4 · 7H 2 O 0.01. First, after activating the preserved Serratia strain to obtain a single colony, inoculate it into a 100ml Erlenmeyer flask containing 30ml of medium, and continuously culture it in a shaker at 30°C at a constant temperature of 140 rpm for 12 hours to obtain a Serratia liquid Seeds: Transfer the liquid seeds to the high-yield liquid medium according to the inoculum size of 1%, and continuously culture them at a constant temperature of 140 r / min in a shaker at 28°C for 6 days to obtain hi...

Embodiment 2

[0022] Bacterial species: Serratia sp., a new strain of Serratia sp. that is highly effective in inhibiting aflatoxin, was isolated from the soil of peanut pods in Huaian City, Jiangsu Province by the Harbin Institute of Technology (Weihai) Marine Science and Technology .

[0023] The medium formula (g / L) used is: colloidal chitin 6, beef extract 5, peptone 10, sodium chloride 8, ammonium sulfate 3, sodium citrate 3, K 2 HPO 4 2. MgSO 4 1. FeSO 4 · 7H 2 O 0.02. First, after activating the preserved Serratia strain to obtain a single colony, inoculate it into a 100ml Erlenmeyer flask containing 30ml of medium, and continuously culture it in a shaker at 30°C at a constant temperature of 140 rpm for 12 hours to obtain a Serratia liquid Seeds: Transfer the liquid seeds to the high-yield liquid medium according to the inoculum size of 5%, and continuously cultivate them at a constant temperature of 140 r / min in a shaker at 30°C for 6 days to obtain high-yield fermentation o...

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Abstract

The invention relates to a serratia plymithica synthetic culture medium and a method for preparing fermentation liquid of the serratia plymithica synthetic culture medium. The formula (gram / liter) of the culture medium comprises the following components of 2 to 10 of colloidalchitin, 3 to 10 of beef extract, 5 to 15 of peptone, 6 to 10 of sodium chloride, 1 to 5 of ammonium sulfate, 1 to 3 of sodium citrate, 1 to 5 of dipotassium phosphate, 0.5 to 3 of magnesium sulfate and 0.01 to 0.05 of ferrous sulfate. The serratia plymithica high-yield fermentation liquid for producing a large quantity of active substances and chitinase which are used for suppressing aflatoxin can be obtained by continuously culturing on a table concentrator at the temperature within 28 and 35 DEG C and the speed of 140 r / min for 4 to 6 days according to the inoculation quantity within 1 to 10 percent. The activity of the chitinase in fermentation supernate is 2.04 to 5.36U / ml; the rate of suppressing the growth of parasitic aspergillus silk is 80.13 to 89.56 percent; and the rate of suppressing the aflatoxin is 90.22 to 98.31 percent. The culture medium is rational in component, and the preparation method is simple and reliable.

Description

technical field [0001] The invention belongs to the field of microbial culture medium and fermentation technology, and relates to a synthetic culture medium for fermented products to efficiently inhibit aflatoxin and simultaneously produce chitinase and its application, in particular to a fermented synthetic culture of Serratia Base and fermentation method. Background technique [0002] Aflatoxins are a class of fungal secondary metabolites with similar structures, which have carcinogenic, teratogenic and mutagenic effects. Aflatoxin contamination of peanuts in my country is very serious, and they are susceptible to aflatoxin contamination in the field and during storage. How to effectively prevent aflatoxin from contaminating peanuts and other agricultural products has very important practical significance for ensuring food safety. [0003] Serratia marcescens is a high-yielding strain of chitinase, and the hydrolysis product of chitinase, chitooligosaccharides, has the f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/42C12R1/425
Inventor 王凯闫培生丁清龙武芹席赵腾飞邵翅
Owner HARBIN INST OF TECH AT WEIHAI
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