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48results about How to "Will not affect the result" patented technology

Use of piperazinopyrimidine isotope labeling reagent

The invention relates to a use of a piperazinopyrimidine isotope labeling reagent. The piperazinopyrimidine isotope labeling reagent is a light / heavy isotope labeling reagent of [d0]- / [d6]-2,4-dimethoxy-6-piperazinopyrimidine. The piperazinopyrimidine isotope labeling reagent can be used for comparison analysis of carboxyl compounds such as fatty acid, polypeptides or proteins. Fatty acid analysis comprises the following steps of a) fatty acid reacts respectively with light and heavy labeling reagents; after after-treatment, an LC-MS / MS analysis process is carried out; and product ions m / z 225 and m / z 231 are selected as quantitative ions; and b) through observation of pairs of mass spectrum peaks of the labeled fatty acid, identification of a plurality of carboxyl fatty acids is realized and quantification is realized according to relative peak areas. Polypeptide / protein comparison analysis comprises the following steps of a) a protein needing to be analyzed is subjected to thermal denaturation and enzymolysis and then reacts with the light / heavy labeling reagent; and b) the reaction solution is subjected to after-treatment; and a MALDI-TOF MS comparison analysis process is carried out.
Owner:SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI

Loop-mediated isothermal amplification detection primer set, method and kit for detection of Xanthomonas oryzae

The invention discloses a loop-mediated isothermal amplification detection primer set, method and kit for detection of Xanthomonas oryzae. Based on genome specific molecular marker characteristics ofXanthomonas oryzae, conservative specific DNA fragment xcp of Xanthomonas oryzae is acquired by means of a comparative genomics method; diseased rice seedlings and seeds are successfully detected through loop-mediated isothermal amplification; the method herein is quick, simple and efficient. The whole detection process has no need for special instruments and can be finished within 45-90 min in afield or lab. The method is high in sensitivity, suitable for detecting rice materials containing down to 10 bacteria, good in specificity, and is free of influence upon results due to other pathogensor plant genomes. The method is good in safety and free of harm and employs no organic agents or nucleic acid binding dyes; all agents are directly releasable to the environment; the method is suitable for detecting all possible pathogen propagating sources.
Owner:HAINAN UNIVERSITY

Watermelon fertilizing method

The invention discloses a watermelon fertilizing method, and belongs to the technical field of fruit fertilizing. The method comprises the following steps: 1, base fertilizer application: applying farmyard manure to soil, adding plant ash and calcium superphosphate, uniformly mixing with soil, covering soil, and planting or directly sowing ; and 2, additional fertilization: carrying out first time fertilizer application after directly sowed sprouts of watermelon seedlings have two true leaves or transplanted sprouts recover, applying urea to the sprouts as a seedling assisting fertilizer when the watermelon sprouts vine, applying a ternary composite fertilizer between the sprouts after the sprouts vine, carrying out trenching urea and potassium sulfate fertilizer application during the appearance of young fruits of the watermelons, and respectively spraying 0.5% potassium dihydrogen phosphate and 0.5% calcium nitrate two times before maturation. Fertilization is carried out according to the difference of nutrients required by the watermelons in different stages, and sufficient nutrients are provided for the watermelon sprouts in each stage in order to make the watermelon sprouts rapidly grow, so high yield and economic benefit improvement are realized.
Owner:黄少伟

Purity evaluation device for electronic-grade trichlorosilane

The invention relates to a purity evaluation device for electronic-grade trichlorosilane. The device comprises a chassis, a bell-jar, which is arranged on the chassis and has a cooling water jacket, a tantalum filament, which is arranged in the bell-jar with the cooling water jacket, and a pair of electrodes, which go through the chassis and are connected to the power supply. The top part of the bell-jar with the cooling water jacket is provided with a hanger and a bell-jar cooling water outlet, the side wall of the bell-jar with the cooling water jacket is provided with a bell-jar cooling water inlet and a sight glass; the two electrodes are connected through the tantalum filament, the outside of the pair of electrodes is provided with a reaction gas inlet and a reaction gas outlet, and the reaction gas inlet and the reaction gas outlet go through the chassis and communicate with the cavity of the bell-jar with the cooling water jacket. The purity evaluation device for electronic-grade trichlorosilane has the advantages of simple structure and low cost, achieves the goals of rapid and precise purity measurement, and realizes safety operation.
Owner:青海黄河上游水电开发有限责任公司新能源分公司 +1

Program-controlled variable five-bit non-reciprocal microwave monolithic integrated attenuator

The invention discloses a program-controlled variable five-bit nonreciprocal microwave monolithic integrated attenuator, which consists of an input port standing wave regulation module, a microwave attenuation module, an output port standing wave regulation module and a control module (21), wherein the input port standing wave regulation module is completely symmetrical with the output port standing wave regulation module. The program-controlled variable five-bit nonreciprocal microwave monolithic integrated attenuator has the advantages that: all attenuation networks are connected in parallel, the loss of microwave signals is low; all control switches and all microwave single-pole single-throw switches are realized by adopting small gallium arsenide field effect transistors, and have high switching performance, and the operation bandwidth of the attenuator is wide; an attenuation circuit is connected in parallel with a microwave amplitude balancing circuit, so the amount of attenuation in the whole working band is balanced and flat; attenuation control coding is irregular, and if a certain bit is shifted, results may not be influenced greatly; and a novel structure is introduced to reduce the sensitivity of a process and then the yield of a chip is increased from 60 percent to 80 percent.
Owner:THE 13TH RES INST OF CHINA ELECTRONICS TECH GRP CORP

A method for extracting and processing vitamin A detection and analysis in food

InactiveCN103852367BOvercome problems such as incomplete water removalOvercoming outcome effectsPreparing sample for investigationBiotechnologyFreeze-drying
The invention belongs to the field of fine chemical extraction, and in particular relates to an extraction treatment method for detection and analysis of vitamin A in food. First, the food sample is freeze-dried to remove water, then the freeze-dried food sample is added to the chloroform-ethanol mixture and homogenized at high speed, then the homogenized sample is centrifuged and the chloroform extract located in the lower layer is collected, and the extract is dried with nitrogen. Ethanol is added to reconstitute and purify the vitamin A, and ultrasonic waves are used in the redissolution step to completely dissolve and extract the vitamin A. The extraction of the present invention does not require processes such as lye saponification and degreasing and vacuum distillation in the traditional method, and also overcomes the problems of incomplete dehydration of food samples caused by adding anhydrous sodium sulfate to grind and absorb water, and has the advantages of simple operation and shortened time , high extraction efficiency, etc., and also overcomes the danger of using ether, n-hexane or methanol as the extraction solvent. Due to the water solubility of ethanol, even trace amounts of water in the extracted sample will not affect the results.
Owner:XIAMEN UNIV

Program-controlled variable four-digit microwave one-chip integrated attenuator

The invention discloses a program-controlled variable four-digit microwave one-chip integrated attenuator. The attenuator is composed of an input port standing wave adjusting module, a microwave attenuation module, an output port standing wave adjusting module and a control module (21); impedances of all the ports are normalized to 50 ohms; and the ports can be utilized in cascade connection to realize high attenuation. The attenuator provided in the invention has the following advantages that: all attenuation networks are in parallel connection, so that there are few losses on a microwave signal; all control switches and all microwave single-pole single-throw switches employ GaAs field effect transistors, which are extremely small, so that the switch performances are good and a working bandwidth of the attenuator is very wide; an attenuation circuit and a microwave amplitude balancing circuit are in parallel connection, so that the attenuation in a whole working frequency band is substantially balanced and flat; there is no rule for attenuation control coding, so that there is no great influence on results caused by deviation of a certain digit; And introduction of a novel structure enables sensitiveness of the technology to be reduced and a chip yield to be improved from 60% to 80%.
Owner:THE 13TH RES INST OF CHINA ELECTRONICS TECH GRP CORP

Experimental measurement device and method for additional mass matrix between circular and cylindrical members of annular fluid region of reactor

The invention provides an experimental measurement device and method for an additional mass matrix between circular and cylindrical members of an annular fluid region of a reactor, simplification and scaling are carried out according to a fast reactor model, and five branch circular bearing cylinders are symmetrically placed in a semi-annular region device with an included angle of 180 degrees relative to a main pump bearing cylinder according to a placement relation in a fast reactor body; the semi-annular region device is filled with water, each bearing cylinder is provided with three layers of measuring points, the measuring points are all underwater, and each layer of measuring points is provided with an acceleration sensor and a pressure sensor for measuring acceleration and pressure. A vibration exciter is used for carrying out vibration excitation on each bearing cylinder, and an additional mass matrix is obtained through calculation according to a formula of fluid acting force borne by a plurality of interacting entities in water. According to the invention, the obtained result is more accurate and stable by selecting the sensors and the measuring point positions in a targeted manner. By adopting the measurement mode of the device, the fluid-solid coupling characteristic between adjacent cylinders in a limited drainage basin can be better evaluated.
Owner:NORTH CHINA ELECTRIC POWER UNIV (BAODING)

Application of mass spectrum technology to detection of molecular level of PSM-E in urine in preparation of product for early diagnosis of prostate cancer

The invention discloses application of A mass spectrum technology to detection of molecular level of PSM-E in urine in preparation of product for early diagnosis of prostate cancer A urine protein sample is diluted and then is directly introduced into a liquid chromatography-electrospray ionization-tandem mass spectrometer for determination, and the content level of the PSM-E in the urine can be rapidly and accurately detected; a fully-synthesized PSM-E characteristic peptide standard substance is adopted as an internal standard quantitative analysis substance, so that errors caused in a sample pretreatment process are reduced; the selectivity and the accuracy of the method are better improved by the liquid chromatography-tandem mass spectrometry; through selection and optimization of a chromatographic column and elution conditions, the chromatographic separation process is improved, and the chromatographic analysis time is shortened. According to the invention, the quantitative detection of the PSM-E in the urine sample can be realized, the operation is simple, the application is convenient, the content of the PSM-E in the urine can be used as a specific biomarker for detecting the prostatic cancer, and an efficient and feasible technical method is provided for early screening of the prostatic cancer.
Owner:广州济士源生物技术有限公司

Program-controlled variable 5-bit reciprocal microwave monolithic integrated attenuator

The invention discloses a program-controlled variable 5-bit reciprocal microwave monolithic integrated attenuator. The program-controlled variable 5-bit reciprocal microwave monolithic integrated attenuator comprises an input port standing wave adjusting module, a microwave attenuating module, an output port standing adjusting module and a control module (27), wherein the input port standing wave adjusting module is completely symmetrical to the output port standing wave adjusting module. The program-controlled variable 5-bit reciprocal microwave monolithic integrated attenuator has the following advantages: the attenuator has very small microwave signal loss because all of attenuation networks are connected in parallel; all of control switches and microwave single-pole single-throw switches have excellent switching performance by adopting small GaAs field effect transistors, and the attenuator has wide working bandwidth; the attenuating circuit is connected with a microwave amplitude balancing circuit in parallel, so that the attenuation in the whole working frequency band is flat and balance; attenuation control codes are irregular so as to obviate large influences on the result when a certain bit offsets; and the introduction of the novel structure can lower the sensitivity to the process, thereby improving the chip yield from 60% to 80%.
Owner:THE 13TH RES INST OF CHINA ELECTRONICS TECH GRP CORP
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