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116results about How to "The result is stable and accurate" patented technology

System, method, and computer program for assessing risk within a predefined market

A system and method for measuring or quantifying the probability of default of a borrower. Credit factors from companies that banks have extended loans to are inputted and collected into a processor. The method employs a process utilizing an optimization function and a standard multivariate nonlinear regression to process client information and to provide an output value whose value is indicative of the likelihood or risk of default by a particular borrower.
Owner:THE MCGRAW HILL

Equipment residual life prediction method and system

The invention belongs to the technical field of mechanical equipment state monitoring and life prediction, and discloses an equipment residual life prediction method and system. The method comprises the steps: firstly, screening historical monitoring signals of multiple physical quantities of equipment need to be acquired, and signals with high relevancy from the historical monitoring signals; setting a normalization label for the screened signal and converting the normalization label into a matrix form; constructing a nested long-short-term memory neural network, and training by using a knownsignal matrix to obtain an equipment residual life prediction model; finally, further optimizing model output through a Gaussian filtering method, and guaranteeing that an output result is stable andreliable. According to the method and the system adopting the method, the residual service life and the degradation state of the equipment, especially the mechanical equipment which works for a longperiod and has a coupling fault mode, can be accurately predicted in real time, the faults of the mechanical equipment can be perceived in advance, and safe, stable and long-term operation of the equipment is guaranteed.
Owner:HUAZHONG UNIV OF SCI & TECH

X-ray controlled contract agent injection

An imaging system including a closed-loop controller (FC) for automatically adapting a power injector system (PJ). The power injector (PJ) injects contrast agent into an object (V) and the imager (100) acquires one or more images (AN, AN i) of the object (V) with the contrast agent resident therein. The controller (FC) calculates a desired optimal injection rate from the images (AN, ANi). Controller (FC) then controls the injector (PJ) according to the calculated optimal injection rate.
Owner:KONINKLJIJKE PHILIPS NV

Long-path infrared spectrometer

A tunable mid-infrared laser operated in a pulsed mode is coupled off-axis into a high-finesse optical cavity to produce a long-path spectrometer. The cavity receives a gas sample. Laser pulses may be wavelength-scanned by stepping an external grating, allowing the grating to mechanically settle, then measuring the ring-down with a set of laser pulses, before moving on the next wavelength. A detector receiving infrared light exiting the cavity supplies a cavity ring-down trace representative of sample absorption of the infrared pulses. A processor determines an absolute absorption spectrum of the gas sample from the ring-down trace and analyzes sample gas composition and trace concentration from that spectrum. The absorption baseline is highly reproducible and stable, improving the accuracy of multivariate fits, and the spectral resolution can be better than 0.001 cm−1 (contingent upon the laser source), allowing for high-resolution measurements of sharp absorption features.
Owner:ABB RES LTD

Fingerprint authentication apparatus having a plurality of fingerprint sensors and method for same

A fingerprint authentication apparatus and method which processes fingerprint data input through a plurality of fingerprint sensors and uses the processed fingerprint data in authentication to thereby increase security and performance. The fingerprint authentication apparatus includes a first sensor configured to acquire first fingerprint data from a user; a second sensor configured to acquire second fingerprint data that is different from the first fingerprint data; and a control unit configured to determine whether or not to authenticate the user based on similarities obtained from the comparison of each of the first fingerprint data and the second fingerprint data with registered fingerprint data.
Owner:SUPREMA INC

Method for automatic detection and tracking of multiple targets with multiple cameras and system therefor

A method for automatically detecting and tracking multiple targets in a multi-camera surveillance zone and system thereof. In each camera view of the system only a simple object detection algorithm is needed. The detection results from multiple cameras are fused into a posterior distribution, named TDP, based on the Bayesian rule. This TDP distribution represents a likelihood of presence of some moving targets on the ground plane. To properly handle the tracking of multiple moving targets with time, a sample-based framework which combines Markov Chain Monte Carlo (MCMC), Sequential Monte Carlo (SMC), and Mean-Shift Clustering, is provided. The detection and tracking accuracy is evaluated by both synthesized videos and real videos. The experimental results show that this method and system can accurately track a varying number of targets.
Owner:NAT CHIAO TUNG UNIV

Intraperitoneal pressure monitoring system and monitoring method

The invention discloses an intraperitoneal pressure monitoring system. The intraperitoneal pressure monitoring system comprises a catheter, a pressure sensor, a urine collection container, a syringe, a monitor, a first three-way switch and a second three-way switch, wherein the connecting end of the catheter is communicated with a first interface of the first three-way switch; the urine collection container is communicated with a second interface of the first three-way switch; a third interface of the first three-way switch is communicated with a first interface of the second three-way switch by a first extension pipe; the syringe is communicated with a second interface of the second three-way switch; the input end of the pressure sensor is communicated with a third interface of the second three-way switch; and the signal output end of the pressure sensor is electrically connected with the signal input end of the monitor. The invention also discloses a method for carrying out pressure monitoring by adopting the intraperitoneal pressure monitoring system. The intraperitoneal pressure monitoring system and monitoring method disclosed by the invention have the advantages that the pressure measuring result is accurate and reliable, the operation is simple and convenient, and the dynamic monitoring is convenient and the like.
Owner:许永华 +1

Method and a device for objects counting

A method and a device for objects counting in image processing includes acquiring the depth image of any one frame; detecting objects according to the depth image; associating the identical object in different frames to form a trajectory; and determining the number of objects according to the number of trajectories. The devices include an acquisition module for acquiring the depth image of any one frame; a detection module for detecting objects according to the depth image; an association module for associating the identical object in different frames to form a trajectory; a determining module for determining the number of objects according to the number of trajectories. The objects are detected according to the depth image. The identical object in different frames is associated to form a trajectory and the number of objects is determined according to the number of trajectories.
Owner:NEC (CHINA) CO LTD

Tongue division and extracting method for tongue diagnosis used colored digital photo of tongue in Chinese traditional medicine

The invention relates to a tongue color image processing technique used to diagnose tongue, wherein the invention is characterized in that: it uses two steps to extract the tongue, that first obtaining the rough profile of tongue, then using two-dimension deformation profile model to accurately cut the image; in the first step, using divide transformation to process the red channel of image, to obtain rouge tongue profile; when processing, first using saturated threshold value to obtain the mark image, and using mark image to minimize the ladder image, to obtain the image need cutting; then using divide transformation method to treat the cut image on the sample-point-gray value curvature of longitudinal section; division transforming to obtain one group formed by the divisions of each longitudinal section; one connecting area of divisions is the tongue area, while its boundary is the tongue rough profile. The experiment has proved that the image result is accurate to apply different people.
Owner:TSINGHUA UNIV

Robust multi-pose fatigue monitoring method based on face shape regression model

The invention relates to a robust multi-pose fatigue monitoring method based on a face shape regression model. According to the robust multi-pose fatigue monitoring method, the face alignment technology is adopted, an existing face alignment method is improved to be used for accurate human eye location according to practical problems in practical application of fatigue monitoring, and the face pose information can be provided at the same time; the stable human eye position can be provided on the condition of complex light environments and different poses of personnel, and the accurate human eye position can be provided even if the personnel turn away; during application of fatigue monitoring, the closing state sequence of the human eyes can be judged preliminarily according to the given human eye area, and the stability and the applicability of a fatigue monitoring product are further improved because the fatigue state of the personnel is judged through the face pose cooperatively; the closing state of the eyes in a front face image can be accurately judged, and the closing state of the eyes in a sideway face image can be judged. The fatigue state is judged through the face pose information cooperatively, and therefore the robustness is further improved, and the fatigue monitoring requirement of the personnel in a complex scene can be met.
Owner:ZHEJIANG ICARE VISION TECH

Measurement method and system for a humidity or gas concentration sensor

A method and system relating to an improvement in measuring accuracy of a gas concentration sensor, the method comprising the steps of measuring a parameter of the sensor, the parameter being a resistance or a capacitance, that is sensitive to the gas or its concentration being measured, and simultaneously changing the temperature of the sensor in a cyclic manner. The parameter of the sensor being measured is measured more than two times or continuously during each heating cycle. The measured parameter value is filtered to obtain a signal component associated with the fundamental frequency of the heating cycle for use at a later step, and the thus filtered signal component is used in the correction and / or calibration of actual output signal of the sensor.
Owner:OYJ VAISALA

Measuring device for liquid sample and method for measuring liquid sample

An objective of this invention is to achieve good measurement accuracy, stable measurement sensitivity and rapid response in a device for measuring a liquid sample and a method for measuring a liquid sample using a sensor comprising an enzyme layer and to minimize sensitivity reduction in repeated or continuous measurement. A device for measuring a liquid sample consists of a sensor comprising an enzyme layer, an upper case 3 and a lower case 4 sandwiching the sensor 5. The sensor 5 is removable from the upper case 3. The upper case 3 comprises a liquid sample inlet channel 1 and a liquid sample outlet channel 2. The liquid sample inlet channel 1 is placed at an angle to the surface of the sensor 5 while its opening end is placed in the vicinity of the surface of the sensor 5.
Owner:NEC CORP

Long-path infrared spectrometer

A tunable mid-infrared laser operated in a pulsed mode is coupled off-axis into a high-finesse optical cavity to produce a long-path spectrometer. The cavity receives a gas sample. Laser pulses may be wavelength-scanned by stepping an external grating, allowing the grating to mechanically settle, then measuring the ring-down with a set of laser pulses, before moving on the next wavelength. A detector receiving infrared light exiting the cavity supplies a cavity ring-down trace representative of sample absorption of the infrared pulses. A processor determines an absolute absorption spectrum of the gas sample from the ring-down trace and analyzes sample gas composition and trace concentration from that spectrum. The absorption baseline is highly reproducible and stable, improving the accuracy of multivariate fits, and the spectral resolution can be better than 0.001 cm−1 (contingent upon the laser source), allowing for high-resolution measurements of sharp absorption features.
Owner:ABB RES LTD

Analysis method for ingredients and mixing ratio of oil product and mixed oil by using terahertz spectroscopy

The invention discloses an analysis method for ingredients and a mixing ratio of an oil product and mixed oil by using terahertz spectroscopy. The method comprises the following steps of: acquiring a terahertz frequency range, and solving a signal sn ( sequential number) (t) of nsi (nitrogen solubility index) (1THz) measured by a plurality of times which is changed along a measurement time; determining a time period t1- t2 of the mixed oil in the measurement time according to the signal sn (t); respectively solving oil product types in pipeline sequence transportation in the 0-t1 time and t2-tend time; carrying out least square fitting on an absorption coefficient alpha si (w) which corresponds to a measuring sequence appeared in the t1-t2 time in the terahertz frequency range, and solving respective volume percentages of two types of the oil products in the corresponding mixed oil. The two mixed oil products decided by the invention are stable and accurate in results and low in misjudgment rate. The mixing ratio obtained by the least square fitting and an actual mixing ratio have an average error which is 4.3%, and oil mixed regions in the oil product pipeline sequence transportation can be precisely cut and divided.
Owner:TIANJIN UNIV

Polynucleotide for identifying male and female ginkgo strains and application thereof

The invention provides a polynucleotide for identifying male and female ginkgo strains. The polynucleotide comprises or consists of: 1) a nucleotide sequence shown as SEQ ID NO: 5; or 2) a complement,degenerate or homologous sequence of the sequence shown as SEQ ID NO: 5; or 3) a polynucleotide which hybridizes to the nucleotide sequence shown in SEQ ID NO: 5 under stringent conditions or a complement thereof; 4) a coding sequence of any of the sequences 1) to 3); or 5) a nucleotide sequence identical to a nucleotide of 10 bp or more in any of the sequences of sequences 1) to 4). The polynucleotide provided by the present invention is unique among male ginkgo plants, uses the same to identify male and female plants of ginkgo, has the advantages of stable and accurate results, good reproducibility and rapid detection, and provides a basis for the identification of male and female ginkgo by molecular markers.
Owner:AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI +1

Clutch friction plate flatness testing equipment and using method thereof

The invention discloses clutch friction plate flatness testing equipment and a using method thereof. The clutch friction plate flatness testing equipment comprises an upper structure and a lower structure. The upper structure comprises an air cylinder support, an air cylinder, a linear bearing, a tested friction plate, a spline seat, a precise sensor support, a fluted disc, a magnetoelectric sensor and an operating console. The lower structure comprises a belt pulley upper end bearing, a control box, a main shaft, a main bearing, a belt pulley III, a triangular belt II, a belt pulley shaft, a belt pulley II, a belt pulley I, a triangular belt I, a motor supporting plate, a belt pulley lower end bearing, motor fixing bolts, a gearbox, an alternating-current motor, a lower support and a frequency converter. A main transmission system, a friction plate bearing mechanism and a precise probe fixture are arranged in the upper structure and the lower structure. The clutch friction plate flatness testing equipment has the characteristics of higher rapidness and simplicity in installation, high automation level, convenience in testing operation and the like, and can better guarantee the accuracy of clutch friction plate flatness testing.
Owner:HANGZHOU DIANZI UNIV

Method for detecting drugs in goat milk and goat milk products

InactiveCN107561190AMake up detection methodMake up for the lack of comprehensive standardsComponent separationOrganic solventInstrumentation
The invention provides a method for detecting 7 classes of 59 forbidden drugs in goat milk and goat milk products. The method comprises the following steps: (1) sample pretreatment: extracting a to-be-detected sample with an acid organic solvent, carrying out purification by virtue of an SPE column, dissolving a purified liquid by virtue of a primary flow phase, and carrying out UPLC-MS / MS detection; and (2) detection of 59 to-be-detected forbidden drugs: detecting the pretreated sample by virtue of an ultra-high performance liquid chromatography-mass spectrometry under specified instrument conditions of the method. The method has the advantages that many drugs can be detected, the type of the drugs is rich, the detection time is short, the sensitivity is high, and a result is accurate andstable; and meanwhile, the detection blank that multiple types and quantity of drugs in the goat milk and the goat milk products can be simultaneously detected is filled up, and the method has important significances to the guarantee of the body health of consumers and the increase of export trade competitiveness of the goat milk and the goat milk products.
Owner:CHINA ANIMAL DISEASE CONTROL CENT

Rapid detection method for health degree of battery module

The invention discloses a rapid detection method for the health degree of a battery module. The method comprises the following steps: acquiring platform voltage data of a battery module sample with known available capacity in a charging and discharging process; establishing a probability density function PDF curve of a known available capacity battery module sample according to the platform voltage data, and calculating a peak area of a set voltage interval; fitting and establishing a battery module health degree SOH-probability density function PDF curve; obtaining the relationship between the voltage data and the battery aging degree in the charging and discharging process of the battery module, wherein only platform voltage data in the charging and discharging process of the to-be-detected battery module needs to be detected in the detection process; and establishing a battery module health degree SOH value of a battery module sample corresponding to the consistency of the peak areain the battery module health degree SOH-probability density function PDF curve and the detection peak area, wherein the battery module health degree SOH value is the battery module health degree SOHvalue of the to-be-detected battery module. The method is simple, the detection speed is high, the battery module does not need to be independently modeled and detected, and the detection result is accurate.
Owner:国网陕西省电力公司汉中供电公司 +3

Kit for quantitative evaluation for long-term recurrent risk of colorectal cancer

The invention relates to functional genomics and a gene expression detection and analysis technology, in particular to a kit for quantitative evaluation for long-term recurrent risk of colorectal cancer. The kit is characterized by consisting of 18 pairs of primers, 18 specific taqman fluorescent probes, 10* reverse transcription-polymerase chain reaction (RT-PCR) buffer solution, deoxyribonucleoside triphosphate (dNTP) mixed liquid, reverse transcriptase, deoxyribose nucleic acid (DNA) polymerase, 10* PCR buffer solution and ribonucleic acid (RNA) enzyme inhibitor. By adopting the self-designed and optimized RT primers and integrating a RT-PCR technology and a taqman real-time fluorescent quantitative PCR technology, the kit is simple and rapid in operation, more stable in diction results and lower in detection cost.
Owner:苏州科贝生物技术有限公司

Detachable probe cover for ear thermometer and manufacturing method thereof

InactiveUS20120076169A1Easy to installNot easily take off a measuring probeThermometer detailsRadiation pyrometryEngineeringEar thermometers
This present invention discloses a detachable probe cover for an ear thermometer and a manufacturing method thereof. The detachable probe cover for the ear thermometer is for being mounted onto a measuring probe of the ear thermometer, wherein a combining mechanism is provided at a bottom of the measuring probe and the detachable probe cover comprises a main body of a hollow structure and a base, in which the main body has an open end and a closed end opposite to the open end, and the hollow structure has a diameter gradually reducing from the open end toward the closed end.
Owner:ACTHERM INC

Fingerprint authentication apparatus having a plurality of fingerprint sensors and method for same

A fingerprint authentication apparatus and method which processes fingerprint data input through a plurality of fingerprint sensors and uses the processed fingerprint data in authentication to thereby increase security and performance. The fingerprint authentication apparatus includes a first sensor configured to acquire first fingerprint data from a user; a second sensor configured to acquire second fingerprint data that is different from the first fingerprint data; and a control unit configured to determine whether or not to authenticate the user based on similarities obtained from the comparison of each of the first fingerprint data and the second fingerprint data with registered fingerprint data.
Owner:SUPREMA INC

Discrimination method for laser damage on the surface of a film or an optical element and determination device thereof

The invention relates to a discrimination method and determination device for laser damage on the surface of a film or an optical element and a determination device therof. The prior art has the disadvantages of strong subjectivity, great working intensity and low testing efficiency. The invention provides a discrimination method for laser damage on the surface of a film or an optical element. According to the method, an operating laser beam carries out monopulse laser irradiation on a sample; if the surface of the sample has no damage, the surface does not produce plasma flash; if the surface is damaged, strong plasma flash is generated the moment the damage appears, and if a photoluminescence peak of an element not being N, O, H or C appears according to analysis of a flash spectrum, appearance of damage can be determined. The invention also discloses a determination device realizing the above-mentioned method. The device comprises a laser generator and a test board and is characterized by further comprising a convergent lens and a fibre-optical probe on the imaging surface of the convergent lens, wherein the fibre-optical probe, a fiber spectrometer and a computer are connected sequentially. According to the invention, high precision in the discrimination is obtained; the discrimination speed is fast; a wide variety of films can be discriminated; the construction of the determination device is simple.
Owner:XIAN TECHNOLOGICAL UNIV

Method for detecting drugs in goat milk and products thereof

The invention provides a method for detecting 8 categories of restricted drugs in goat milk and products thereof, wherein the number of the restricted drugs is 74. The method comprises: (1) pre-treating a sample: extracting a sample to be detected with an acid organic solvent, purifying with a SPE column, and dissolving the purified liquid by using an initial mobile phase so as to be used for detection with UPLC-MS / MS; and (2) determining 74 restricted drugs: determining the pre-treated sample by using ultra performance liquid chromatography-LC-MS under the instrument condition specified by the method. According to the present invention, the method has advantages of more types of detected drugs, rich variety, short detection time, high sensitivity and accurate and stable results, can makesup for the absence of the simultaneous detection of multiple drugs in goat milk and dairy products, and provides the important significance for the assurance of the health body of consumers and the improvement of the export competitiveness of goat milk and dairy products.
Owner:CHINA ANIMAL DISEASE CONTROL CENT

Cloud analysis technology based ovulation tester and testing method thereof

The invention disclosed a cloud analysis technology based ovulation tester and a testing method thereof. The cloud analysis technology based ovulation tester comprises a device terminal and a cloud computing server; the device terminal comprises a terminal body, a terminal body control unit, a sensing probe and a lithium battery power; the terminal body control unit comprises a temperature detection unit connected with a temperature calculation module, a conductivity detection module connected with a conductivity calculation module, a data compression module, a data transmission module and a data receiving module, and the data compression module, the data transmission module and the data receiving module are connected in sequence; the temperature calculation module and the conductivity calculation module are respectively connected with the data compression module; the terminal body control unit is associated with the cloud computing server through the data transmission module and the data receiving module; the sensing probe is connected with the temperature detection module and the conductivity detection module; the cloud computing server is provided with an information feature extraction module, an information feature matching module, a trend judgment module and a sending module which all connected in sequence. With the method, outcome prediction is more stable.
Owner:南京市小鲸鲨信息科技有限公司

Ultrasonic water meter interception coefficient processing method

The invention relates to the technical field of ultrasonic water meter detection, in particular to an ultrasonic water meter interception coefficient processing method. The method includes A, extracting a calculation value of a last interception coefficient in memory; B, extracting a time difference acquired by the current detection in the memory; C, acquiring a current interception coefficient Pn according to the step A and step B; D, judging relations between the Pn with a minimum Pmin and a maximum Pmax, and writing an appropriate value into the memory; E, judging the relations between the Pn with a preset threshold P1 and P2, and entering different flow processing programs. By guiding in the interception coefficient, according to the associations of the current detecting time with the last interception coefficient, by means of the feature that the flow of a fluid in the ultrasonic water meter never change suddenly, the current detecting time difference can be judged, and different flow processing programs are entered according to different judging results, so that the calculated result is more accurate, and initiative flow performance indexes of flow detection can be improved greatly.
Owner:姜跃炜

Method and a device for objects counting

A method and a device for objects counting in image processing includes acquiring the depth image of any one frame; detecting objects according to the depth image; associating the identical object in different frames to form a trajectory; and determining the number of objects according to the number of trajectories. The devices include an acquisition module for acquiring the depth image of any one frame; a detection module for detecting objects according to the depth image; an association module for associating the identical object in different frames to form a trajectory; a determining module for determining the number of objects according to the number of trajectories. The objects are detected according to the depth image. The identical object in different frames is associated to form a trajectory and the number of objects is determined according to the number of trajectories.
Owner:NEC (CHINA) CO LTD

Method and device for testing gelation time of electrolyte colloid

The invention discloses a method for testing the gelation time of electrolyte colloid, which comprises the following steps of: providing a gelation time test object with large specific gravity; leading the test object to obtain initial velocity; causing the test object obtaining the initial velocity to fall into a tested liquid electrolyte, wherein the test object in the liquid electrolyte receives the combined action of gravity, buoyancy and electrolyte resistance; promoting the resistance (F) to the test object in the solidification process, wherein the time for the test object to deceleratefrom the initial velocity to zero under the action of the electrolyte resistance is the gelation time of the liquid electrolyte. The invention adopts the test object with large specific gravity, thusnot only overcoming the influence of buoyancy factors, but also providing larger impulse force for the test object, and obviously showing resistance change in the gelation process of the electrolyte,and further determining the gelation time according to the change of the motion state of the test object, providing reference for the infusion time of colloidal batteries, solving the problem of half-way gelation during infusion of the colloidal batteries by controlling the time for infusing the electrolyte, and guaranteeing the quality of the colloidal batteries.
Owner:ZHAOQING LEOCH BATTERY TECH

Panoramic image and video splicing method, computer-readable storage medium, and panoramic camera

The present invention is applicable to the fields of panoramic images and videos, and provides a panoramic image and video splicing method, and a panoramic camera. In the present invention, fisheye photos captured by two adjacent cameras are mapped to corresponding seam areas of a sphere model, so as to form two strip graphs having overlapping areas; performing block template matching on the two strip graphs to obtain an initial template matching result; performing matching filtering on the initial template matching result by using an area expansion-based matching filtering algorithm, so as to obtain a final matching result; and updating a mapping relationship between the fisheye photos and the corresponding seam areas of the sphere model, and performing panoramic splicing according to the updated mapping relationship to obtain a seamless panoramic image.
Owner:SHENZHEN ARASHI VISION CO LTD

A detection kit for measuring small berry orac and its detection method

InactiveCN102269702AGood repeatabilitySolving Kinetic Lag Time Measurement ProblemsFluorescence/phosphorescenceCardboardDisodium Fluorescein
The invention relates to a method for measuring the total antioxidant capacity of small berries, provides an ORAC detection kit suitable for the determination of the total antioxidant capacity of small berries and a detection method thereof, and belongs to the technical field of activity detection and determination. The kit includes: antioxidant standard substance Trolox storage reagent, fluorescent substance fluorescein sodium storage reagent, free radical generator AAPH reagent, phosphate buffer, and fluorescent microwell reaction plate, which is made of a plastic or hard paper material Boxes are packed together to make up. The ORAC detection method for the total antioxidant capacity of small berries comprises the following steps: small berry sample pretreatment; detection with reagent kits; and analysis of detection results. By mixing the sample and the reagent in a certain volume ratio, the fluorescence microplate analyzer is used to detect the change of the fluorescence intensity of the complex, thereby measuring and calculating the total antioxidant capacity of the small berry. The invention can provide a complete set of total antioxidant capacity test of small berries, has high sensitivity and good accuracy, and is convenient for popularization and application.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Method for detecting acetyl choline content in peripheral blood mononuclear cells

The invention provides a method for detecting the acetyl choline content in peripheral blood mononuclear cells. The method provided by the invention lays a foundation for accurately detecting the ACh (acetyl choline) content by separating an ACh ingredient from a sample to be detected by adopting a UPLC (ultra performance liquid chromatography) technology. According to the technical scheme, an HILIC (hydrophilic interaction chromatography) column is found to be more suitable for separation of a polar compound by adopting experimental measures, can be used for effectively separating ACh from iso-ACh, and has a better separation effect on Cho. On this basis, chromatographic conditions and internal standard substance categories are further designed, and d9-ACh of isotope is taken as national standard substances of ACh and iso-ACh at the same time to furthest reduce the analysis error caused by sample damage and effectively ensure the accuracy of a measurement result. According to the method provided by the invention, on the basis of effectively separating ACh by using the UPLC, ACh signal detection is performed by a liquid chromatography-mass spectrometry system, and the ACh is accurately quantified by optimizing mass spectrometer conditions with a stable and accurate result and favorable linearity and sensitivity; meanwhile, the method has relatively low detection limit and relatively high recovery rate and also has a broad application prospect.
Owner:GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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