The invention relates to a
surface modified carbon nano point, preparation thereof, application of the
surface modified carbon nano point used as a
fluorescence probe for detecting Cu2+ and
glutathione. The invention relates to a detection method of the Cu2+ and the
glutathione, and aims to solve the technical problems that an existing
fluorescence probe for detecting the Cu2+ is easy to interfere by other
metal ions, high in preparation cost and complex in preparation step. The triethylene
tetramine-
modified carbon nano point is used as the
fluorescence probe for detecting the Cu2+ and the
glutathione; the preparation method of the fluorescence probe comprises the steps of
microwave heating
citric acid and
urea in a water solution, adding the acquired carbon nano point into an
ethanol solution containing triethylene
tetramine for reflowing, and preparing the fluorescence probe. The preparation method is simple, the
reaction conditions are moderate, and the cost is low. A detection method comprises the steps of fluorescence
quenching TCDs and forming a TCDs-Cu2+ compound through interaction of the fluorescence probe and the Cu2+, further interacting the TCDs-Cu2+ compound and the glutathione, recovering fluorescence of the TCDs, and detecting the Cu2+ and the glutathione. The probe can be used for detecting
copper ions and the glutathione in the water solution and a life entity.