The invention discloses a method for continuously massively producing
recombinant adenoviruses. The method comprises the following steps: 1,
cell culture; 2,
cell culture bag culture; 3, large-scale amplification of the adenoviruses; and 4, purification, wherein
cell culture bags in step 2 and step 3 are placed on a shaking table, and culture in a thermotank is carried out; and the culture conditions of the
cell culture bags are as follows: the temperature is 37 DEG C, the pH value is 7.2, the shaking speed is 10-20 rpm, and the angle of the shaking table is 7-9 DEG. The
cell growth environment is improved, damages of ventilation to cells are reduced, and the dissolved
oxygen effect is increased, so the problems of difficult
digestion, separation and transfer of cells in traditional technologies are solved, thereby the enlargement culture problem and the
continuous production problem of adherent cells are solved, and the problem of difficult freeze-thaw
cracking of the cells is solved;and the adherent cells are cultured in the
cell culture bags filled with a sheet carrier and the shaking
bed, so the damages of a mechanical shearing force to the cells are reduced, and the growth density of the cells is improved, thereby the
total cell yield of the cells is improved, and the yield of the adenoviruses is increased.