Fermentation method for erythromycin
A fermentation method and tetracycline technology, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of increasing the difficulty of fermentation process operation, incomplete utilization of delayed carbon sources, waste of raw materials, etc. The effect of shortening the bacteria time, reducing the amount of starch, and reducing the cost of raw materials
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Embodiment 1
[0025] 1.1 Seed culture: Inoculate the slant spores prepared by conventional methods into the sterilized seed medium for cultivation.
[0026] The composition of seed medium: starch 2.5%, soybean powder 2.5%, yeast powder 0.5%, corn steep liquor 0.5%, ammonium sulfate 0.3%, light calcium carbonate 0.3%, sodium bromide 0.2%, and the rest is water. Prepare and sterilize according to the ratio of weight to volume.
[0027] Culture conditions: culture temperature 32°C, ventilation rate 1: 0.4 v / v / min with stirring, culture for 22 hours to obtain seed liquid.
[0028] 1.2 Fermentation culture: Inoculate the seed liquid into the sterilized fermentation medium with 18% inoculum amount for cultivation.
[0029] Composition of fermentation medium: starch 3.0%, dextrin 1.5%, glucose 1.5%, soybean powder 2.0%, corn steep liquor 2.0%, ammonium sulfate 0.5%, calcium carbonate 0.5%, sodium bromide 0.2%, M-accelerator 0.002% , and the rest is water. Prepare and sterilize according to the ...
Embodiment 2
[0038] 2.1 Seed culture: Inoculate the slant spores prepared by conventional methods into the sterilized seed medium for seed culture.
[0039] The composition of seed medium: starch 4.0%, soybean powder 3.5%, yeast powder 1.5%, corn steep liquor 1.0%, ammonium sulfate 0.7%, light calcium carbonate 0.5%, sodium bromide 0.35%, and the rest is water. Prepare and sterilize according to the ratio of weight to volume.
[0040] Culture conditions: culture temperature 35°C, ventilation rate 1: 0.8v / v / min with stirring, cultivate for 27.8hr to obtain seed liquid.
[0041] 2.2 Fermentation culture: Inoculate the seed liquid into the sterilized fermentation medium according to 24% inoculation amount to carry out fermentation sheet culture.
[0042] Composition of fermentation medium: starch 4.5%, dextrin 2.5%, glucose 2.5%, soybean powder 3.0%, corn steep liquor 3.0%, ammonium sulfate 0.8%, calcium carbonate 0.7%, sodium bromide 0.35%, M-accelerator 0.003% , and the rest is water. Pr...
Embodiment 3
[0051]3.1 Seed culture: Inoculate the slant spores prepared by conventional methods into the sterilized seed medium for seed culture.
[0052] The composition of seed medium: starch 3.5%, soybean powder 3.0%, yeast powder 1.0%, corn steep liquor 0.8%, ammonium sulfate 0.5%, light calcium carbonate 0.4%, sodium bromide 0.3%, and the rest is water. Prepare and sterilize according to the ratio of weight to volume.
[0053] Culture conditions: culture temperature 33°C, ventilation rate 1: 0.8v / v / min with stirring, cultivate for 26hr to obtain seed liquid.
[0054] 3.2 Fermentation culture: Inoculate the seed liquid into the sterilized fermentation medium according to 20% inoculum amount for fermentation culture.
[0055] Composition of fermentation medium: starch 4.0%, dextrin 2.0%, glucose 2.0%, soybean powder 2.5%, corn steep liquor 2.5%, ammonium sulfate 0.7%, calcium carbonate 0.6%, sodium bromide 0.3%, M-accelerator 0.0015% , and the rest is water. Prepare and sterilize ac...
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