The invention discloses a method for culturing non-heading Chinese cabbage
microspore plantlets. The method comprises the following specific steps of (1) flower
bud selection, wherein flower buds withthe number ratio of 1:1 between petals and anthers are selected; (2) culture of
microspore derived embryos, wherein the flower buds collected in the step (1) are disinfected by a
sodium hypochloritesolution for 10-15 minutes, washed with sterilization water and rinsed by an NLN-13 culture medium; the rinsed flower buds are completely ground, filtered, centrifuged, diluted by the NLN-13 culture medium containing 10-30 mg / L of a
boron substance and subpackaged in culture dishes,
activated carbon is added,
heat shock treatment is conducted, dark culture is conducted at 24-26 DEG C for 14-16 days, and the embryoids are formed; (3)
germination into the plantlets from mature embryos, wherein the embryoids are subjected to shake culture, mature embryos turning to green are transferred into a
solid MS culture medium for culture, and after 38-42 days of culture,
acclimatization and transplant can be directly conducted. According to the method, on the premise of ensuring the
embryo formation rate, the operation steps are simplified, the
operation time is greatly shortened, and the
plantlet production efficiency is improved.