The invention discloses a RS232 communication interface circuit that supports hot swapping. The interface circuit uses a high-speed operational amplifier as a core device, and is equipped with corresponding peripheral devices to form a voltage buffer; a three-stage tube is used as an inverter, and the phase is reversed twice consecutively. , obtain the same phase transmission level, and also realize the wide voltage range of the interface circuit, meet the requirements of the RS232 communication frequency band, realize the hot swap of the serial port circuit, and meet the voltage requirements between different devices and different signal levels. The power supply circuit of the interface circuit can follow the input voltage, and can overcome the surge voltage and surge current during the hot swapping process. The invention has high integration degree, small volume, simple circuit connection mode and low failure rate. In the case of failure, it can also facilitate fault detection and analysis, and troubleshoot and repair. The invention can be used in the transmission of different signal levels of each RS232, and has level conversion, signal buffering, hot plug function and high reliability.