The invention relates to a reverse dismantling method for a high-rise frame structure, and belongs to the field of building construction. A reverse dismantling method for a high-rise frame structure comprises the following steps that S10, wall structures of the first layer and the second layer are dismantled, beam plates of the second layer are dismantled, frame columns are all dismantled, and the frame columns are replaced with steel columns; s20, the concrete beams on the second layer are dismantled, the main beams are replaced with the steel beams, a plurality of jacking devices are fixed to the steel beams at intervals in the steel beam replacing process, and the jacking devices are used for jacking and supporting the main beams on the third layer; s30, the pushing device is driven to contract, the high-layer frame structure descends, frame columns on the second layer fall into the operation space, and the frame columns on the second layer are dismantled; s40, wall structures and beam plates of the third layer are dismantled, concrete beams of the third layer are dismantled, a jacking device is used for jacking and supporting main beams of the fourth layer, a pushing device is driven to contract, and frame columns of the third layer are dismantled; and according to the step S40, the high-rise frame structure is dismounted layer by layer. The dismantling method for the high-rise frame structure is high in dismantling speed, environmentally friendly and low in labor intensity.