36results about How to "Enhance growth-promoting effect" patented technology

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and / or improved chemical stability, e.g. as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and / or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and / or a deletion of one or more of amino acids 31 to 33 and / or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy.

The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoter and the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.

The invention provides the production of plant growth-promoting compound microbial agent based on the ecological relationship among bacterial strains and its application. A plant growth-promoting composite bacterial agent, which is mixed with the bacterial liquid of Bacillus amyloliquefaciens SQR9, Bacillus amyloliquefaciens RF‑CU‑1, Bacillus lysinica RF‑CU‑2 and Bacillus brevifaciens RF‑CU‑3 made. The bacillus synthetic flora of the invention can cooperate with each other and significantly promote the growth of cucumber plants, and the growth-promoting effect is also significantly improved with the increase of the diversity of the inoculated flora under the condition of aseptic hydroponic culture in the laboratory.

The invention discloses a nitrilase gene nit2 and its overexpression method and application. A key gene for IAA biosynthesis in Trichoderma harzianum NJAU4742 is a nitrilase gene nit2, the nucleotide sequence of which is shown in SEQ ID NO.1. A method for overexpression containing the nitrilase gene nit2. Application of nitrilase gene nit2 and its overexpression fragment in constructing Trichoderma harzianum NJAU4742 genetically engineered bacteria with significantly improved IAA yield. The newly discovered nit2 gene in the Trichoderma harzianum NJAU4742 of the present invention plays an important role in the IAA synthesis process, and the overexpression of the gene can significantly increase the IAA content at the pericycle in the root hair region of the main root system of the plant. The invention provides a theoretical basis and technical support for the development of novel trichoderma bio-organic fertilizer and the development of ecological agriculture.

The invention belongs to the field of soil remediation, and in particular relates to a method for improving severely saline-alkali land. Specifically, a new Bacillus subtilis is provided, and a modifier is prepared by using it. The specific components of the modifier include: 5-10 parts of microbial inoculum, 70-80 parts of organic fertilizer, 10-20 parts of biochar, desalination 5 to 10 doses. And based on the improver, a series of methods for improving saline-alkali land are provided. Using the method provided by the invention has no secondary pollution and is environmentally friendly, and can completely improve the severely saline-alkali land into land that can be normally cultivated.

The invention discloses a strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion. The invention provides a strain of Pseudomonas fluorescens pf27, and prepares a microbial agent pf27. Experiments have shown that the plant height and root length of plants are significantly improved after the microbial agent pf27 treatment, and it has a certain growth-promoting effect on the plants. Up to 829.14%, it has a broad-spectrum and high-efficiency role in promoting plant growth, especially can promote the growth of Solanaceae vegetable crops and improve their quality. The invention solves the problems of poor effect or residues of other methods such as chemical fertilizers, and helps to promote the sustainable development of agriculture, and has a good application prospect.