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A strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion

A Pseudomonas fluorescens, plant technology, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve problems such as hindering crop transformation and absorption, soil nutrient imbalance, crop nutrition imbalance, etc., to promote sustainable agriculture. Development, efficient plant growth, the effect of promoting plant growth

Active Publication Date: 2019-07-02
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Excessive application of several chemical fertilizers of a single nature will easily lead to nutritional imbalance of crops. Most of the chemical fertilizers contain various salts, among which chemical substances such as nitrogen, phosphorus, and potassium are easily consolidated by the soil and accumulate in the soil. The salinization of the soil causes the imbalance of soil nutrients, affects and hinders the transformation and absorption of the absorbed nutrients by crops, makes the growth characteristics of fruits and vegetables inferior, the taste and quality of crops deteriorate, and the quality declines. For example, vegetables taste unsavory and fruits taste bad Not sweet, poor taste, and perishable, not suitable for storage

Method used

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  • A strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion
  • A strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion
  • A strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The isolation and identification of embodiment 1, pf27 bacterial strain

[0054] 1. Isolation of pf27 strain

[0055] pf27 was isolated from the rhizosphere soil of the wild rice field in Pu'er City, Yunnan Province (22 N. 。 33'56", 100 。 35'12", 737 meters above sea level). The specific separation method is as follows: pick the root system from the rhizosphere soil in the verrucous wild rice area, scrape the rhizosphere soil, weigh 5g, and put it into the prepared In a sterilized Erlenmeyer flask, dilute to 10 with sterilized water -6 times, set 3 repetitions. Take 200 μl of the soil samples that were diluted in gradient and spread them on MEA medium, beef extract peptone medium, LB medium and KB medium plates respectively, culture them in 28°C and 37°C incubators, and observe the growth status of the colonies every day. The result shows a dilution of 10 -5 、10 -6 There were basically no colonies growing on the plate when doubled, and the dilution was 10 -4 When...

Embodiment 2

[0066] Embodiment 2, the preparation of microbial bacterial agent pf27

[0067] The Pseudomonas fluorescens pf27 obtained in Example 1 was shaken and cultured in KB medium, shaken and cultured at 28°C and 200rpm for 12-16h, then centrifuged at 4000rpm for 20min, discarded the supernatant, and collected the bacterial cell precipitate , and then dilute the thalline precipitation with sterilized water to make microbial agent pf27 (bacteria agent pf27), the total concentration of pf27 viable bacteria in the finished product of microbial agent pf27 is 1×10 9 -1×10 10 CFU / mL.

Embodiment 3

[0068] Embodiment 3, the growth-promoting effect of microbial bacterial agent pf27 on tomato seedlings of Solanaceae crops

[0069] 1. Experimental method

[0070] Tomato seeds Zhongza No. 9 (produced by Zhongshu Seed Industry Technology (Beijing) Co., Ltd.) were sterilized with 3% NaClO solution for 10 minutes, and ddH 2 O was washed 6 times to obtain sterilized seeds. Then spread the sterilized seeds evenly on the sterilized vermiculite peat soil matrix (mix the vermiculite and peat soil according to the ratio of 1:1, autoclave at 121°C, 20min), and germinate at 26°C, 12h:12h light: Cultivate the seeds to germinate under dark conditions, and when the seeds germinate and grow to two true leaves, they are transplanted into the evenly added bacterial agent pf27 (using a concentration of 50ml of bacterial solution with an OD value of 1 for each kg of vermiculite peat soil matrix mixture, The final concentration of bacterial solution was 1×10 5CFU / mL) and the nutrient holes of...

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Abstract

The invention discloses a strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion. The invention provides a strain of Pseudomonas fluorescens pf27, and prepares a microbial agent pf27. Experiments have shown that the plant height and root length of plants are significantly improved after the microbial agent pf27 treatment, and it has a certain growth-promoting effect on the plants. Up to 829.14%, it has a broad-spectrum and high-efficiency role in promoting plant growth, especially can promote the growth of Solanaceae vegetable crops and improve their quality. The invention solves the problems of poor effect or residues of other methods such as chemical fertilizers, and helps to promote the sustainable development of agriculture, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion. Background technique [0002] The continuous cropping obstacles brought about by intensive production have seriously affected a series of problems such as crop yield and quality, plant physiological characteristics, soil microbial community structure, and rampant plant diseases and insect pests, seriously affecting the planting efficiency and healthy development of agriculture. The large-scale excessive application of chemical fertilizers has caused great harm to the environment and human health, and has become an important factor affecting the environment, human health and social development. In recent years, due to the large-scale production and unlimited use of chemical pesticides and chemical fertilizers, they have polluted the human living environment and agricultural, forestry an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/39
CPCA01N63/00C12N1/20C12N1/205C12R2001/39
Inventor 叶健姚香梅王端
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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