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A kind of vibrio lx6-2 and its application in preparing biological seaweed fertilizer

A technology of seaweed fertilizer and vibrio, applied in microorganism-based methods, microorganisms, microorganisms, etc., can solve problems such as the blank of industrialization technology, the lack of in-depth systematic research on the development process and application mechanism, etc.

Active Publication Date: 2020-06-09
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of seaweed fertilizer by biological fermentation is limited to laboratory research at home and abroad, and there is a lack of in-depth systematic research on its development process and application mechanism, and it is still blank in terms of industrialization technology.

Method used

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  • A kind of vibrio lx6-2 and its application in preparing biological seaweed fertilizer
  • A kind of vibrio lx6-2 and its application in preparing biological seaweed fertilizer
  • A kind of vibrio lx6-2 and its application in preparing biological seaweed fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Embodiment 1: the preparation of biological seaweed fertilizer of the present invention

[0104] The implementation steps of this embodiment are as follows:

[0105] A. Incline culture of Vibrio LX6-2

[0106] Prepare slant medium: prepare pH7.0 containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate, 2g / l dipotassium hydrogen phosphate and 20g / l agar powder aqueous solution, and then sterilized at a temperature of 121° C. for 30 minutes to obtain the slant culture medium;

[0107] Inoculate the glycerol tube strain of Vibrio LX6-2 strain on the slant surface of the test tube slant solid medium, and then culture the slant surface in a constant temperature incubator at a temperature of 28°C for 2 days to obtain a slant culture;

[0108] B. Seed culture

[0109] Preparation of seed medium: prepare a pH7.0 aqueous solution containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate and 2g / l dipotass...

Embodiment 2

[0116] Embodiment 2: the preparation of biological seaweed fertilizer of the present invention

[0117] The implementation steps of this embodiment are as follows:

[0118] A. Incline culture of Vibrio LX6-2

[0119] Prepare slant medium: prepare pH7.0 containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate, 2g / l dipotassium hydrogen phosphate and 20g / l agar powder aqueous solution, and then sterilized at a temperature of 121° C. for 30 minutes to obtain the slant culture medium;

[0120] Inoculate the glycerol tube strain of Vibrio LX6-2 strain on the slant surface of the test tube slant solid medium, and then culture the slant surface in a constant temperature incubator at a temperature of 29°C for 3 days to obtain a slant culture;

[0121] B. Seed culture

[0122] Preparation of seed medium: prepare a pH7.0 aqueous solution containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate and 2g / l dipotass...

Embodiment 3

[0129] Embodiment 3: the preparation of biological seaweed fertilizer of the present invention

[0130] The implementation steps of this embodiment are as follows:

[0131]A. Incline culture of Vibrio LX6-2

[0132] Prepare slant medium: prepare pH7.0 containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate, 2g / l dipotassium hydrogen phosphate and 20g / l agar powder aqueous solution, and then sterilized at a temperature of 121° C. for 30 minutes to obtain the slant culture medium;

[0133] Inoculate the glycerol tube strain of Vibrio LX6-2 strain on the slant surface of the test tube slant solid medium, and then culture the slant surface in a constant temperature incubator at a temperature of 30°C for 2 days to obtain a slant culture;

[0134] B. Seed culture

[0135] Preparation of seed medium: prepare a pH7.0 aqueous solution containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate and 2g / l dipotassi...

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Abstract

The invention relates to a Vibrio LX6‑2 and its application in preparing biological seaweed fertilizer. The preparation step of the biological seaweed fertilizer includes the steps of Vibrio LX6‑2 slant culture, seed cultivation, preparation of seaweed digestive juice and fermentation of the biological seaweed liquid. The gibberellin content of the biological seaweed fertilizer of the present invention is more than 5000ppm, which is 1-2 times higher than conventional chemical treatment methods and other biological methods, and can effectively promote plant growth and increase crop yield. The composite biological seaweed fertilizer of the present invention also has the effect of promoting the formation of aggregate structure in the soil, enhancing the soil permeability, and has the function of obviously improving the drought resistance and stress resistance of plants, and has a remarkable effect of promoting growth. It has a good control effect on Rhizoctonia graminearum and Rhizoctonia solani and so on.

Description

【Technical field】 [0001] The invention belongs to the technical field of biological fertilizers. More specifically, the present invention relates to a Vibrio LX6-2, and also relates to the use of the Vibrio LX6-2 in preparing biological seaweed fertilizer. 【Background technique】 [0002] Seaweed is a rich source of marine organisms, widely used in food, industrial raw materials, and herbal medicines. Seaweed has been used in agriculture and livestock production for centuries. Seaweed fertilizer is a new type of pure natural fertilizer with no toxic and side effects. It is rich in amino acids, minerals, polysaccharides, vitamins and physiologically active substances. It can increase crop yield, improve quality and enhance crop drought resistance, cold resistance, disease resistance, etc. effect. Therefore, the use of seaweed to produce organic fertilizers has become one of the important hotspots in the research of new fertilizers. [0003] Seaweed fertilizer is made from ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C05F17/20C05G5/23C05G3/00C05G3/60C05G3/80C12R1/63C12R1/125C12R1/07C12R1/39C12R1/10
CPCC05B7/00C05F17/00C05G3/00C12N1/20C12P1/04C05G3/80C05G5/23C12N1/205C12R2001/07C12R2001/10C12R2001/125C12R2001/39C12R2001/63C05C11/00C05D5/00C05D9/00C05F11/00C05F11/08Y02W30/40
Inventor 孙杉杉肖艳杜迎辉高小佳杨娜李丽艳揣峻峰徐延平
Owner 领先生物农业股份有限公司
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