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Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof

A technology of arbuscular mycorrhizal fungi and bio-fertilizers, applied in the fields of application, rice cultivation, fertilizer mixture, etc., can solve the problems of arbuscular mycorrhizal fungus infection, rice cannot obtain mineral nutrition, difficult symbiosis, etc., and achieve a good ecological environment , Improving the distribution of rice materials, and the effect of high rice yield

Active Publication Date: 2013-10-23
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the current problem that rice cannot be infected by arbuscular mycorrhizal fungi in aquatic conditions, and it is difficult to effectively form symbiosis, so that rice cannot obtain mineral nutrition from arbuscular mycorrhizal fungi. Preparation and Application of Compound Biological Fertilizer for Rice Based on Mycorrhizal Fungi

Method used

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  • Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof
  • Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof
  • Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof

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specific Embodiment approach 1

[0013] Specific embodiment one: present embodiment is based on the preparation of the composite rice special-purpose biological fertilizer of arbuscular mycorrhizal fungus, according to the following steps: one, the preparation of colonization reinforcement: 1kg white clover seed adds 1.5kg water and 0.001kg molybdenum Ammonium acid, soaked for 12 hours, rinsed with distilled water for 3 to 5 times, and obtained after drying;

[0014] 2. Pretreatment of the arbuscular mycorrhizal fungal agent: cut the white clover roots in the arbuscular mycorrhizal fungal agent to 1~2cm, crush the matrix in the arbuscular mycorrhizal fungal agent and pass through a 10-mesh soil sieve , and the treated arbuscular mycorrhizal fungus inoculum is obtained after the two are mixed evenly;

[0015] 3. Mix the treated arbuscular mycorrhizal fungal agent, rice seedling strengthening agent and colonization enhancer according to the volume ratio of (1500~2500):(300~600):1, and then complete the arbuscul...

specific Embodiment approach 2

[0018] Specific embodiment two: what this embodiment is different from specific embodiment one is the preparation of the arbuscular mycorrhizal fungus inoculum in the step 3, carry out according to the following steps: one, field soil, river sand and vermiculite cross 10 mesh soil sieves respectively , and then mix field soil, river sand and vermiculite in a volume ratio of 2:5:3 as a substrate, sterilize the substrate by high-pressure steam at 121°C for 2 hours, wash and dry the flower pots, and then use 0.1% high manganese Potassium acid potassium solution for 30 minutes; 2. Soak white clover seeds in alcohol with a volume concentration of 75% for 10 minutes, then rinse 3 times with distilled water, and accelerate germination at 28°C; 3. Put 6 kg of sterilized Substrate, spray sterile water until the water seeps from the base of the pot, inoculate the substrate with arbuscular mycorrhizal fungi at an inoculation rate of 20 spores per pot, and then evenly sprinkle with germina...

specific Embodiment approach 3

[0020] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the processed arbuscular mycorrhizal fungal agent, rice seedling strengthening agent and colonization enhancer are mixed in a volume ratio of 2000:450:1 in step 3 . Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoter and the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.

Description

technical field [0001] The invention relates to the preparation and application of special biological fertilizer for rice. Background technique [0002] Nitrogen and phosphorus are essential macroelements for rice growth, and the lack of nitrogen and phosphorus will cause poor growth of crops, eventually leading to a sharp decline in rice yield and quality. Fertilizer use has contributed significantly to the growth of rice globally today. However, the increase in rice yield that relies heavily on chemical fertilizers comes at the expense of the ecological environment. Rice has a low utilization rate of chemical fertilizers, and unabsorbed nutrients cause environmental pollution through leakage, surface runoff, and denitrification. In addition, greenhouse gases from nitrogen fertilizer production and its application on farmland, such as CO 2 , N 2 O and NO x , and have an increasing impact on global climate change. At the same time, the over-exploitation of phosphate ro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00A01G16/00A01G22/22
Inventor 王立张淑娟马放张雪李哲
Owner HARBIN INST OF TECH
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