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Vibrion LX6-2 and application thereof in preparation of biological seaweed fertilizer

A technology of seaweed fertilizer and vibrio, which is applied in the directions of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems such as the gap in industrialization technology, the lack of in-depth systematic research on the development process and application mechanism, etc.

Active Publication Date: 2017-07-07
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of seaweed fertilizer by biological fermentation is limited to laboratory research at home and abroad, and there is a lack of in-depth systematic research on its development process and application mechanism, and it is still blank in terms of industrialization technology.

Method used

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  • Vibrion LX6-2 and application thereof in preparation of biological seaweed fertilizer
  • Vibrion LX6-2 and application thereof in preparation of biological seaweed fertilizer
  • Vibrion LX6-2 and application thereof in preparation of biological seaweed fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Embodiment 1: the preparation of biological seaweed fertilizer of the present invention

[0108] The implementation steps of this embodiment are as follows:

[0109] A. Incline culture of Vibrio LX6-2

[0110] Prepare slant medium: prepare pH7.0 containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate, 2g / l dipotassium hydrogen phosphate and 20g / l agar powder aqueous solution, and then sterilized at a temperature of 121° C. for 30 minutes to obtain the slant culture medium;

[0111] Inoculate the glycerol tube strain of Vibrio LX6-2 strain on the slant surface of the test tube slant solid medium, and then culture the slant surface in a constant temperature incubator at a temperature of 28°C for 2 days to obtain a slant culture;

[0112] B. Seed culture

[0113] Preparation of seed medium: prepare a pH7.0 aqueous solution containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate and 2g / l dipotass...

Embodiment 2

[0120] Embodiment 2: the preparation of biological seaweed fertilizer of the present invention

[0121] The implementation steps of this embodiment are as follows:

[0122] A. Incline culture of Vibrio LX6-2

[0123] Prepare slant medium: prepare pH7.0 containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate, 2g / l dipotassium hydrogen phosphate and 20g / l agar powder aqueous solution, and then sterilized at a temperature of 121° C. for 30 minutes to obtain the slant culture medium;

[0124] Vibrio LX6-2 strain glycerol tube strains were inoculated on the slant of the test tube slant solid medium, and then cultured on the slant in a constant temperature incubator at a temperature of 29°C for 3 days to obtain a slant culture;

[0125] B. Seed culture

[0126] Preparation of seed medium: prepare a pH7.0 aqueous solution containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate and 2g / l dipotassium hydrogen...

Embodiment 3

[0133] Embodiment 3: the preparation of biological seaweed fertilizer of the present invention

[0134] The implementation steps of this embodiment are as follows:

[0135] A. Incline culture of Vibrio LX6-2

[0136] Prepare slant medium: prepare pH7.0 containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate, 2g / l dipotassium hydrogen phosphate and 20g / l agar powder aqueous solution, and then sterilized at a temperature of 121° C. for 30 minutes to obtain the slant culture medium;

[0137] Inoculate the glycerol tube strain of Vibrio LX6-2 strain on the slant surface of the test tube slant solid medium, and then culture the slant surface in a constant temperature incubator at a temperature of 30°C for 2 days to obtain a slant culture;

[0138] B. Seed culture

[0139] Preparation of seed medium: prepare a pH7.0 aqueous solution containing 5g / l sodium alginate, 3g / l peptone, 3g / l sodium chloride, 0.1g / l magnesium sulfate and 2g / l dipotass...

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Abstract

The invention relates to vibrion LX6-2 and application thereof in preparation of a biological seaweed fertilizer. The preparation steps of the biological seaweed fertilizer are as follows: slant cultivation of the vibrion LX6-2, seed cultivation, preparation of seaweed digestive juice, fermentation of the biological seaweed liquid and the like. The content of gibberellins of the biological seaweed fertilizer is not less than 5000ppm; compared with the conventional chemical treatment method and other bio-treatment methods, the content of the gibberellins is improved by 1 to 2 times, so that plant growth development is effectively promoted and the crop yield is increased. The composite biological seaweed fertilizer disclosed by the invention also has a soil improvement effect in promoting soil to form pellets and enhancing soil permeability, has functions of obviously improving drought-resistant and stress-tolerant ability of plants and obviously promoting plant growth, has a good control effect on take-all disease of wheat and bacterial wilt of pepper, and has a good prevention and treatment effect on rhizoctonia cerealis, rhizoctonia solani and the like.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of biological fertilizers. More specifically, the present invention relates to a Vibrio LX6-2, and also relates to the use of the Vibrio LX6-2 in preparing biological seaweed fertilizer. [0003] 【Background technique】 [0004] Seaweed is a rich source of marine organisms, widely used in food, industrial raw materials, and herbal medicines. Seaweed has been used in agriculture and livestock production for centuries. Seaweed fertilizer is a new type of pure natural fertilizer with no toxic and side effects. It is rich in amino acids, minerals, polysaccharides, vitamins and physiologically active substances. It can increase crop yield, improve quality and enhance crop drought resistance, cold resistance, disease resistance, etc. effect. Therefore, the use of seaweed to produce organic fertilizers has become one of the important hotspots in the research of new fertilizers. [0005] Seaweed fertilize...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C05G3/00C05G3/04C05F17/00C12R1/63C12R1/125C12R1/07C12R1/39C12R1/10C05G3/80
CPCC05B7/00C05F17/00C05G3/00C12N1/20C12P1/04C05G3/80C05G5/23C12N1/205C12R2001/07C12R2001/10C12R2001/125C12R2001/39C12R2001/63C05C11/00C05D5/00C05D9/00C05F11/00C05F11/08Y02W30/40
Inventor 孙彬彬肖艳杜迎辉高小佳杨娜李丽艳揣峻峰徐延平
Owner 领先生物农业股份有限公司
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