The invention discloses a radiation-proof plant fermentation beverage and a manufacturing method applied in the technical field of food. The formula of the beverage consists of algae, grape skin and seeds, concentrated black tea juice, blueberry, edible alcohol, high fructose syrup, xylitol, honey and water. The manufacturing method produces the beverage with radiation-proof function and special application by extracting the algae, the grape skin and seeds, the concentrated black tea juice and the blueberry juice through alcohol and implementing acetic acid fermentation. The manufacturing method comprises the following steps of: soaking and cleaning the algae by using warm water to remove impurities such as sand and the like and salt, and preparing algae size for later use; mixing the grape skin and seeds, the concentrated black tea juice and the blueberry juice in the algae size, adding the edible alcohol, and performing normal temperature extraction; inoculating acetic acid bacteria to the regulated solution, and performing deodorization, curing and precipitation treatment; and filtering the fermented solution, adding a proper amount of the high fructose syrup, the xylitol, the honey and the water to prepare the radiation-proof plant fermentation beverage, and filling the beverage. The beverage has very high iodine supplementing efficiency and safety, is rich in proanthocyanidin and tea polyphenol, is easily absorbed by the human body, and can relieve the social problem caused by nuclear radiation after being frequently drunk.