The invention discloses a Macrobrachium rosenbergii Nodavirus RT-LAMP-LFD (reverse transcription-loop-mediated isothermal amplification-lateral flow dipstick) detection kit and a detection method thereof. The detection method includes: according to the conserved region of a Macrobrachium rosenbergii Nodavirus (MrNV) sequence published by GenBank, designing the primers and probe needed by an MrNVRT-LAMP-LDF reaction system; employing a virus RNA extraction reagent prepared by the inventor to extract MrNV virus RNA, using the MrNVRT-LAMP reaction system established by the invention to perform detection, and according to the detection probe added to the reaction system, utilizing nucleic acid rapid test paper to determine the result at the end of the reaction. Compared with common detection technologies of the virus, the method provided in the invention has the characteristics of simplicity and rapidness, good specificity, and high sensitivity, can be used for field service of technical personnel and farmers on the production front line, and is also in favor of diagnosis and prevention of white tail disease.