The invention discloses an isolated kidney perfusion system, and relates to the technical field of kidney transplantation. According to the invention, a liquid conveying pipeline is used as a main body; a liquid inlet pipe, a liquid dripping bucket, a fluid speed regulation roller and an outflow assembly are sequentially arranged on the conveying pipeline in series; and through arrangement of theabove-mentioned structures, the isolated kidney perfusion system provided by the invention can make up the defect that a perfusion device used in the kidney transplantation process in the prior art isgenerally a self-made simple device and is inconvenient when a plurality of blood vessels are encountered or the blood vessels are thin. During kidney perfusion, a medicine can be injected through above-mentioned devices; a flow speed can be adjusted through a pulley; a pressure can be generated during liquid conversion and extrusion through a soft ball to facilitate perfusion; a proper siliconetube can be selected for perfusion according to the thickness and number of kidney arteries; thus, the isolated kidney perfusion system provided by the invention has the advantages of simple structure, widely-available materials, convenient operation, low price, practicality, sufficient perfusion, scientific and reasonable structure and safe and convenient use.