43 results about "Tumor marker test" patented technology

A combined device for rapidly and quantitatively detecting multiple tumor markers is characterized by being composed of 3 to 8 pieces of independent tumor marker test paper such as AFP, CEA, PSA, CA125, CA153, CA199, CA242 and NSE, a clamping shell special for the 3 to 8 pieces of test paper, and an immune layer result analysis and judgment recorder with built-in corresponding standard curves and the judgment method. The test paper can be combined to form the detection device with the 3 to 8 pieces of test paper according to the specific requirements. The standard curves and the judgment method for the 8 pieces of test paper are built in the immune layer result analysis and judgment recorder and used for conducting quantitative judgment on test results, and forecasting of various tumor occurrence conditions can be conducted on different types of combination of the test results through the judgment method. Detection is completed within 15 minutes to 20 minutes. The combined device for rapidly and quantitatively detecting the multiple tumor markers has the advantages of being combined in detection, easy and convenient to operate, rapid in response, accurate in result, suitable for on-site test and the like, and the requirements for more accurate, easier and move convenient clinical detection are met.

The invention discloses a photoelectrochemical immunosensor for detecting CA125, a preparation method and application thereof; on the basis of the principle that PDA can quench CdTe fluorescence on anelectrode substrate, by using of a SiO2@PDA nanocomposite as a probe molecule formed by self-polymerization deposition of dopamine DA and coating with SiO2 and using of CdTe as a photoelectrically-active substance, the photoelectrochemical immunosensor is constructed to detect tumor markers. According to the method, the photoelectrochemical immunosensor is constructed by use of the SiO2@PDA nanocomposite, and is used to detect the tumor markers, compared with an enzyme linked immunosorbent assay, a traditional optical method and the like, the photoelectrochemical immunosensor has the advantages of simple operation, low technical requirement and low price and easy miniaturization, and by use of fluorescence quencher SiO2@PDA, loading of biomolecules can be increased, and a signal can be amplified.

The invention provides a novel method for monitoring the cancer cell number of a cancer patient by adopting circulating DNA. According to the novel method, tumor DNA fragments in plasma of the cancer patient are separated and extracted by adopting a specific free small fragment DNA extraction kit; the obtained tumor DNA fragments are taken as a template, a tumor susceptible gene personalized molecular probe is designed for carrying out QPCR detection on the gene copy number, the detection periods of the tumor patients are different, range from 2 weeks to 1 month, and are determined according to the chemotherapy periods of patients. As the convenient, non-invasive, repeatable and highly-sensitive liquid biopsy technology, the detection method can be applied to various tumor susceptible genes and cancers, and belongs to an important assessment means for the cancer treatment effects, drug resistance and recurrent risk in advance of the clinical tumor marker detection.

The invention discloses a paper chip enzyme-linked immunoassay test card for a combined multi-tumor marker test. The test card is characterized in that upper, middle and lower filter paper chips, a double-sided tape and a paper-based slide frame are arranged between an upper shell and a lower shell; the upper, middle and lower filter paper chips refer to wax patterned filter paper chips which are provided with hydrophilic and hydrophobic regions and are alternately arranged respectively; a sample introduction and liquid feeding window is formed in an upper shell body; the upper filter paper chip is arranged between the upper shell and the double-sided tape; the middle filter paper chip is arranged between the double-sided tape and the lower filter paper chip, and sensitive regions which are distributed in arrays and used for fixing different reaction reagents are arranged on the middle filter paper chip; the water-based slide frame is arranged between the lower filter paper chip and the lower shell; the upper and middle filter paper chips are bonded through the double-sided tape; the lower filter paper chip slides on the water-based slide frame relative to the upper and middle filter paper chips, so that the different reaction reagents can sequentially flow among the multiple layers of filter paper chips. According to the test card, an early diagnosis device applied in clinical site is provided for patients.

The invention discloses a method for detecting a tumor marker through a novel biochemical sensor which is constructed based on integration of a biological probe and DNA (Deoxyribose Nucleic Acid) circle amplification. The method comprises the following steps: carrying out connection modification on the surface of a magnetic ball through hairpin DNA and a precursor 1, adding telomerases for forming hairpin structures and further bonding with gold nanoparticles which are modified by a precursor 2 and marked with a large quantity of fluorescent molecules, and enhancing fluorescent signals through circle amplification; and finally, quantitatively analyzing telomerase in tumour cells by using the fluorescent signals so as to detect the tumor cells as soon as possible in medicine.

The invention discloses a white light-emitting up-conversion nanoparticle and a test paper strip based on the white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers. According to the present invention, the nanoparticle has a three-layer core-shell structure, and emits white light under the excitation of near infrared light; and after the antibodies of multi-component tumor markers to be detected are labeled with the nanoparticle, the obtained conjugates are immobilized on the labeling complex binding zone of the test paper strip, such that the multiple tumor markers can be simultaneously detected; and with the nanoparticle and the test paper strip, the sensitive detection on the multiple tumor markers by the single up-conversion nanoparticle is achieved, and the advantages of simple operation, good specificity, high qualitative and quantitative real-time detection and high sensitivity are provided.

The invention discloses a Prussian blue-based electrochemical immunosensor, an electrochemical immunosensing method built based on the sensor and application. The electrochemical immunosensor is composed of a glassy carbon electrode serving as a base electrode, and a glycan-Prussian blue nanocomposite, gold nanoparticles and a tumor marker capture antibody which are modified on the surface of thebase electrode in sequence. The electrochemical immunosensor is combined with a urease functionalized silicon nanoprobe to determine the content of a tumor marker in a sample. The Prussian blue-basedelectrochemical immunosensor has high analysis properties such as simple preparation process, controllability, high stability, high sensitivity, wide linear range and low cost, and has an extremely practical prospect in the field of clinical tumor marker detection.

The invention discloses a reversibly assembled and disassembled Strep-tag polypeptide-labeled biomolecule derivatization matrix and an application thereof. Polypeptide can be linked on the surfaces of different matrixes on the basis of the characteristic that Strep-tag polypeptide can be bound to a matrix loaded with Strep-Tactin protein. Chemical derivatization is carried out on biological targeting molecules so as to form a biomolecule-Strep-tag conjugate; by virtue of specific recognition effects of the Strep-tag and the Strep-Tactin, the biomolecule-Strep-tag conjugate is immobilized on the surface of the matrix. After biotin is added, the linkage between the biomolecule and the matrix is destroyed due to the competitive binding between the biotin and the Strep-Tactin, so that reversible trapping and releasing on a to-be-detected object are achieved. The matrix is simple and rapid in preparing and using processes, good in repeatability, and is widely applicable to such fields of tumor marker detection, cell trapping and releasing, and the like.

The invention discloses a multichannel field effect transistor nano biosensor and a preparation method and application thereof, and belongs to the field of analysis and detection. According to the multi-channel field effect transistor nano biosensor, the corresponding relation between the bladder cancer marker concentration and an indium gallium zinc oxide (IGZO) field effect transistor sensing device electric signal is established, so that a plurality of bladder cancer markers in a urine sample can be rapidly detected at the same time. The field effect transistor nano biosensor can realize simultaneous rapid detection of a plurality of cancer markers in a urine sample, and well makes up for the defects in the current bladder cancer diagnosis field. The sensor has the advantages of high detection flux, high sensitivity, good selectivity, high detection speed, good reproducibility and the like, and has a wide clinical application prospect in the field of tumor marker detection.

The present invention provides a giant magneto-impedance effect biosensor preparation method for serum tumor marker detection. The method comprises the following steps: (1) adopting a MEMS process to prepare a giant magneto-impedance effect sensor; (2) preparing an aluminum oxide insulation layer on the giant magneto-impedance effect sensor, and carrying out spin photoresist coating, exposuring, developing, aluminum oxide etching, and photoresist removing, such that the sensor pin is exposed; (3) carrying out Cr / Au film sputtering, and carrying out spin photoresist coating, exposuring, developing, Cr / Au film etching, and photoresist removing, such that the Au film on the sensor pin and the sensor sensitive part is exposed; (4) preparing a biologically sensitive film on the Au film; (5) immobilizing tumor marker monoclonal antibody; (6) carrying out antigen spotting; (7) immobilizing biotinylated antibody; and (8) immobilizing a magnetic tag. According to the present invention, the GMI effect sensor is applied in serum tumor marker detection, the detection is rapid, mass production is easily achieved, and the existing clinical application requirements can be met.

The invention discloses a micro RNA (miRNA) biomarker combination and kit for diagnosing or assisting in diagnosing gastric cancer, and relates to the technical field of gastric cancer detection. ThemiRNA biomarker combination comprises miR-18a, miR-93, miR-146b, miR-181a, miR-181b, and miR-335, provides a new way for screening of early gastric cancer, and is superior to an existing tumor markerin the aspects of accuracy and sensitivity of detection.

The invention discloses a tumor risk multiple calculation method and system based on a blood examination index, and belongs to the field of medical data processing. The system comprises a data obtaining module, which is used for obtaining a blood routine laboratory sheet picture, a blood biochemical laboratory sheet picture and a tumor marker laboratory sheet picture, identifying detection indexes, age and gender in the blood routine test sheet picture, the blood biochemical test sheet picture and the tumor marker test sheet picture, or directly importing related data from electronic data; and a tumor risk multiple calculation module, which is used for predicting the increase multiple of the tumor disease risk of the to-be-detected person relative to the average crowd risk, wherein the calculation process of the tumor risk multiple calculation module comprises the steps that classified learning samples are established for non-tumor people and tumor disease people, the learning samples are used for training a tumor disease risk model, the tumor disease risk model is used for an average population sample to calculate an average population tumor disease risk value, and the tumor risk value of the to-be-detected person is divided by the average group tumor risk value to obtain a tumor risk increasing multiple. The tumor risk multiple is predicted by using blood routine examination, blood biochemistry and tumor marker detection indexes, and a tumor risk early warning mode is innovated.

The present invention provides a preparation method of a miniaturized magnetic fluxgate biosensor for serum tumor marker detection. The method comprises the following steps: (1) adopting a MEMS process to prepare a miniaturized magnetic fluxgate sensor on a glass substrate; (2) carrying out Cr / Au film sputtering on the glass substrate, and carrying out spin photoresist coating, exposuring, developing, Cr / Au film etching, and photoresist removing, such that the Au film is exposed; (3) preparing a bio-sensitive film on the Au film; (4) immobilizing tumor marker monoclonal antibody; (5) carrying out antigen spotting; (6) immobilizing biotinylated antibody; and (7) immobilizing a magnetic tag. According to the present invention, the miniaturized magnetic fluxgate biosensor is applied in serum tumor marker detection, the detection is rapid, mass production is easily achieved, and the existing clinical application requirements can be met.

The invention discloses a gastric cancer biomarker based on peripheral blood, a detection method and application thereof. The invention also discloses the detection of peripheral blood RNA m 6 A reagent or kit for diagnosing gastric cancer. The specific peripheral blood RNA m provided by the present invention 6 A. The intermediate results of gastric cancer diagnosis can be used alone or in combination with related mRNA or serum tumor marker detection, which will help physicians to diagnose gastric cancer in combination with clinical symptoms, medical history or other examination information.

The invention discloses a micro-fluidic chip for tumor marker detection and a detection method. The chip comprises a reaction layer substrate and a micro-channel sample adding layer located on the reaction layer substrate, and the reaction layer substrate comprises a glass slide substrate and a graphene oxide quantum dot nanometer material substrate assembled on the glass slide substrate; a strip-shaped detection band and a quality control band are printed on the graphene oxide quantum dot nano material substrate; a plurality of micro-channel grooves are formed in the bottom of the micro-channel sample adding layer, the plurality of micro-channel grooves and the plurality of strip-shaped detection bands and quality control bands are vertically arranged to form a plurality of reaction dot matrixes, and an inflow hole and an outflow hole which are through up and down are further formed in the micro-channel sample adding layer; and two ends of the micro-channel groove are respectively communicated with the inflow hole and the outflow hole. According to the chip and the detection method disclosed by the invention, multiple different tumor markers can be detected at the same time, and the chip and the detection method have the advantages of rapidness, stability and on-site high-throughput detection.

The invention relates to the field of image recognition, and discloses a medical image recognition method and device based on tumor markers, equipment and a medium, and the method comprises the steps: cutting a first region of interest in a to-be-recognized medical image, and obtaining a global to-be-recognized image; cutting a second region of interest in the to-be-identified medical image to obtain a local to-be-identified image, the second region of interest being in the first region of interest; performing image recognition on the global to-be-recognized image by using a pre-constructed first recognition model to obtain a first recognition result; performing image recognition on the local to-be-recognized image by using a pre-constructed second recognition model to obtain a second recognition result; and performing comprehensive identification and judgment according to the first identification result, the second identification result and the tumor marker detection result to obtain a target identification result. According to the invention, the problem of low medical image recognition accuracy can be solved.

The present invention provides a preparation method of a miniaturized magnetic fluxgate biosensor for serum tumor marker detection. The method comprises the following steps: (1) adopting a MEMS process to prepare a miniaturized magnetic fluxgate sensor on a glass substrate; (2) carrying out Cr / Au film sputtering on the glass substrate, and carrying out spin photoresist coating, exposuring, developing, Cr / Au film etching, and photoresist removing, such that the Au film is exposed; (3) preparing a bio-sensitive film on the Au film; (4) immobilizing tumor marker monoclonal antibody; (5) carrying out antigen spotting; (6) immobilizing biotinylated antibody; and (7) immobilizing a magnetic tag. According to the present invention, the miniaturized magnetic fluxgate biosensor is applied in serum tumor marker detection, the detection is rapid, mass production is easily achieved, and the existing clinical application requirements can be met.

The invention discloses a photoelectrochemical immunosensor for detecting CA125, a preparation method and application thereof; on the basis of the principle that PDA can quench CdTe fluorescence on anelectrode substrate, by using of a SiO2@PDA nanocomposite as a probe molecule formed by self-polymerization deposition of dopamine DA and coating with SiO2 and using of CdTe as a photoelectrically-active substance, the photoelectrochemical immunosensor is constructed to detect tumor markers. According to the method, the photoelectrochemical immunosensor is constructed by use of the SiO2@PDA nanocomposite, and is used to detect the tumor markers, compared with an enzyme linked immunosorbent assay, a traditional optical method and the like, the photoelectrochemical immunosensor has the advantages of simple operation, low technical requirement and low price and easy miniaturization, and by use of fluorescence quencher SiO2@PDA, loading of biomolecules can be increased, and a signal can be amplified.

The antigen specificity of the antibody that recognizes a glycan antigen and is used as a tumor marker is not sufficient, and it has been a drawback that tumor marker detection using such an antibody is low in detection specificity, thereby deteriorating accuracy of cancer diagnosis.To solve the object described above, the present invention provides an antibody specifically reactive with a glycan of DUPAN-2 antigen for use as a tumor marker, and a production method thereof.

The invention discloses a preparation method and application of a red phosphorus / gold nanoflower composite material. The preparation method is characterized by comprising the following steps: (1) preparing nano red phosphorus by adopting a hydrothermal method; (2) preparing red phosphorus / gold nanoparticles by adopting an in-situ deposition method; and (3) preparing the red phosphorus / gold nano-flower composite material by adopting an anisotropic growth strategy: sequentially adding 1ml of the red phosphorus / gold nano-particle aqueous solution prepared in the step (2), 5ml of a 4-mercaptobenzoic acid solution and 12.5 ml of an ascorbic acid solution into 2.5 ml of a chloroauric acid solution, slightly shaking, enabling the mixture to react for 30 minutes, then washing with deionized water and ethanol for several times, and removing the modified 4-mercaptobenzoic acid molecules by photocatalysis to obtain the red phosphorus / gold nanoflower composite material. The red phosphorus / gold nanoflower composite material can be used for preparing a sandwich immune structure for detecting tumor markers, and has the advantage that the detection sensitivity of the tumor markers is improved.