43 results about "Tumor marker test" patented technology

The invention discloses a method for detecting a tumor marker through a novel biochemical sensor which is constructed based on integration of a biological probe and DNA (Deoxyribose Nucleic Acid) circle amplification. The method comprises the following steps: carrying out connection modification on the surface of a magnetic ball through hairpin DNA and a precursor 1, adding telomerases for forming hairpin structures and further bonding with gold nanoparticles which are modified by a precursor 2 and marked with a large quantity of fluorescent molecules, and enhancing fluorescent signals through circle amplification; and finally, quantitatively analyzing telomerase in tumour cells by using the fluorescent signals so as to detect the tumor cells as soon as possible in medicine.

The invention belongs to the technical field of medical devices, and specifically relates to a tumor marker detection kit, which includes a box body, an upper cover is rotatably mounted on the upper side of the box body, and the upper cover can be rotated within a range of 180°; the box body A placement frame is installed in the body; storage holes are provided on the placement frame; the placement frames in the box body are connected together from left to right through hinge blocks; The hinged blocks are respectively located on both sides of the placement frame; the box body is located at the center line of the box body length direction and a connecting block is installed; the connecting block is fixedly connected with the hinged block located in the middle of the box body; the left and right sides of the box body There are mutually symmetrical pull-outs on the sides of both sides; side covers are installed on the pull-outs of the box, and the side plates can be rotated within 90°; the invention has a simple structure, can place multiple detection reagents at the same time, and A quick search for reagents can be achieved.

The invention discloses a tumor risk multiple calculation method and system based on a blood examination index, and belongs to the field of medical data processing. The system comprises a data obtaining module, which is used for obtaining a blood routine laboratory sheet picture, a blood biochemical laboratory sheet picture and a tumor marker laboratory sheet picture, identifying detection indexes, age and gender in the blood routine test sheet picture, the blood biochemical test sheet picture and the tumor marker test sheet picture, or directly importing related data from electronic data; and a tumor risk multiple calculation module, which is used for predicting the increase multiple of the tumor disease risk of the to-be-detected person relative to the average crowd risk, wherein the calculation process of the tumor risk multiple calculation module comprises the steps that classified learning samples are established for non-tumor people and tumor disease people, the learning samples are used for training a tumor disease risk model, the tumor disease risk model is used for an average population sample to calculate an average population tumor disease risk value, and the tumor risk value of the to-be-detected person is divided by the average group tumor risk value to obtain a tumor risk increasing multiple. The tumor risk multiple is predicted by using blood routine examination, blood biochemistry and tumor marker detection indexes, and a tumor risk early warning mode is innovated.

The invention provides a preparation method of Ru nanoparticles, explores its electrochemical activity, and uses its electrochemical signal to construct a sensor for detecting tumor markers, which belongs to the field of electrochemical applications. The present invention mainly reveals that the Ru nanomaterial has its unique electrochemical oxidation peak; explores the change of the electrochemical oxidation peak of the Ru nanomaterial under light conditions, and uses this characteristic to design an electrochemical sensor for detecting tumor markers . The invention is simple and easy to implement, and has high detection sensitivity.

The invention discloses a gastric cancer biomarker based on peripheral blood, a detection method and application thereof. The invention also discloses the detection of peripheral blood RNA m 6 A reagent or kit for diagnosing gastric cancer. The specific peripheral blood RNA m provided by the present invention 6 A. The intermediate results of gastric cancer diagnosis can be used alone or in combination with related mRNA or serum tumor marker detection, which will help physicians to diagnose gastric cancer in combination with clinical symptoms, medical history or other examination information.

The invention provides a preparation method of Ru nanoparticles and research on electrochemical activity of the Ru nanoparticles, and belongs to the field of electrochemical application, wherein a sensor for detecting tumor markers is constructed by utilizing electrochemical signals of the Ru nanoparticles. The invention mainly discloses that a Ru nano material has a unique electrochemical oxidation peak; and the change of the electrochemical oxidation peak of the Ru nano material under an illumination condition is explored, and the electrochemical sensor for detecting the tumor markers is designed by utilizing the characteristic. The method is simple and easy to implement and has high detection sensitivity.

The invention discloses a method for detecting a tumor marker through a novel biochemical sensor which is constructed based on integration of a biological probe and DNA (Deoxyribose Nucleic Acid) circle amplification. The method comprises the following steps: carrying out connection modification on the surface of a magnetic ball through hairpin DNA and a precursor 1, adding telomerases for forming hairpin structures and further bonding with gold nanoparticles which are modified by a precursor 2 and marked with a large quantity of fluorescent molecules, and enhancing fluorescent signals through circle amplification; and finally, quantitatively analyzing telomerase in tumour cells by using the fluorescent signals so as to detect the tumor cells as soon as possible in medicine.

The invention relates to a tumor marker detection kit based on force coding and application of the tumor marker detection kit. The tumor marker detection kit comprises one or more nano sensors, magnetic beads and a solid phase substrate coupled with or fixed to the nano sensors; and each nano sensor comprises a nucleic acid aptamer, a force coding sequence and a complementary DNA sequence. The tumor marker detection kit based on force coding detects a target by detecting magnetic signals, compared with traditional fluorescence signals, interference of ambient light and the solution color is avoided, and detection is sensitive; and the kit can simultaneously detect a plurality of unmarked tumor markers, and the problems that different proteins have the large concentration difference in vivoand cannot be clinically detected are overcome. In addition, the kit can further detect small molecules, nucleic acid and the like besides detecting the proteins and has wide application prospects.

The present invention provides a preparation method of a miniaturized magnetic fluxgate biosensor for serum tumor marker detection. The method comprises the following steps: (1) adopting a MEMS process to prepare a miniaturized magnetic fluxgate sensor on a glass substrate; (2) carrying out Cr / Au film sputtering on the glass substrate, and carrying out spin photoresist coating, exposuring, developing, Cr / Au film etching, and photoresist removing, such that the Au film is exposed; (3) preparing a bio-sensitive film on the Au film; (4) immobilizing tumor marker monoclonal antibody; (5) carrying out antigen spotting; (6) immobilizing biotinylated antibody; and (7) immobilizing a magnetic tag. According to the present invention, the miniaturized magnetic fluxgate biosensor is applied in serum tumor marker detection, the detection is rapid, mass production is easily achieved, and the existing clinical application requirements can be met.

The present invention relates to a method for detecting pancreatic cancer by using a new tumor marker. Specifically, the present invention provides a method for detecting pancreatic cancer, which includes determining the antibody that is directed against the CAPRIN‑1 protein through the antigen in a sample isolated from a subject. The antibody response has the presence or amount of bound reactive polypeptides and provides a reagent or kit for detecting pancreatic cancer comprising CAPRIN-1 protein or fragments thereof, antibodies directed thereto, or polynucleotides encoding them.

An objective of the invention is, when cancer determination is performed by machine learning using mass spectrum data on a sample taken from a subject, to ensure independence from the determination bya tumor marker test performed in parallel and to increase the reliability of mutual diagnosis. The m / z value of ions derived from substances, used in other tests, such as tumor markers is stored in atarget m / z value DB (31), and a spectrum information filtering unit (22) deletes the signal intensity of the m / z value stored in the target m / z value DB (31) from a large number of mass spectra thathave been classified according to the presence or absence of cancer. A learning processing unit (23) obtains the learning result information by using the data after the deletion as learning data and stores the learning result information in a learning result DB (32). The signal intensity of the predetermined m / z value is likewise deleted also with regard to the mass spectrum data relating to the target sample to be determined, and then a determination processing unit (24) determines the classification into the class corresponding to the presence or absence of cancer on the basis of the learning result information stored in the learning result DB (32).

The invention discloses a photoelectrochemical immunosensor for detecting CA125, a preparation method and application thereof; on the basis of the principle that PDA can quench CdTe fluorescence on anelectrode substrate, by using of a SiO2@PDA nanocomposite as a probe molecule formed by self-polymerization deposition of dopamine DA and coating with SiO2 and using of CdTe as a photoelectrically-active substance, the photoelectrochemical immunosensor is constructed to detect tumor markers. According to the method, the photoelectrochemical immunosensor is constructed by use of the SiO2@PDA nanocomposite, and is used to detect the tumor markers, compared with an enzyme linked immunosorbent assay, a traditional optical method and the like, the photoelectrochemical immunosensor has the advantages of simple operation, low technical requirement and low price and easy miniaturization, and by use of fluorescence quencher SiO2@PDA, loading of biomolecules can be increased, and a signal can be amplified.

The present invention provides a giant magneto-impedance effect biosensor preparation method for serum tumor marker detection. The method comprises the following steps: (1) adopting a MEMS process to prepare a giant magneto-impedance effect sensor; (2) preparing an aluminum oxide insulation layer on the giant magneto-impedance effect sensor, and carrying out spin photoresist coating, exposuring, developing, aluminum oxide etching, and photoresist removing, such that the sensor pin is exposed; (3) carrying out Cr / Au film sputtering, and carrying out spin photoresist coating, exposuring, developing, Cr / Au film etching, and photoresist removing, such that the Au film on the sensor pin and the sensor sensitive part is exposed; (4) preparing a biologically sensitive film on the Au film; (5) immobilizing tumor marker monoclonal antibody; (6) carrying out antigen spotting; (7) immobilizing biotinylated antibody; and (8) immobilizing a magnetic tag. According to the present invention, the GMI effect sensor is applied in serum tumor marker detection, the detection is rapid, mass production is easily achieved, and the existing clinical application requirements can be met.

The invention discloses a white light-emitting up-conversion nanoparticle and a test paper strip based on the white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers. According to the present invention, the nanoparticle has a three-layer core-shell structure, and emits white light under the excitation of near infrared light; and after the antibodies of multi-component tumor markers to be detected are labeled with the nanoparticle, the obtained conjugates are immobilized on the labeling complex binding zone of the test paper strip, such that the multiple tumor markers can be simultaneously detected; and with the nanoparticle and the test paper strip, the sensitive detection on the multiple tumor markers by the single up-conversion nanoparticle is achieved, and the advantages of simple operation, good specificity, high qualitative and quantitative real-time detection and high sensitivity are provided.

The invention discloses a tumor marker detection kit based on variable lymphocyte receptors (VLR) and a method for preparing the same, wherein the tumor marker detection kit is high in detection sensitivity, convenient to store and transport, long in period of validity and low in production cost. Just like the existing tumor marker detection kit, the tumor marker detection kit provided by the invention also contains a trapping reagent and a detection reagent; however, the difference is that the trapping reagent and the detection reagent are recombinant variable lymphocyte receptors having high specificity and high affinity to the tumor marker, respectively; and therefore, the clinical detection of the tumor marker is more simple and convenient and early diagnosis of cancer is more accurate and effective.