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Method for monitoring cancer cell number of cancer patient by adopting circulating DNA

A tumor cell and patient technology, applied in the field of monitoring the number of tumor cells, can solve the problems of the intermediate data detection method and the unsatisfactory effect, etc.

Inactive Publication Date: 2017-02-01
程澎 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current detection methods and effects of these intermediate data are not ideal.

Method used

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  • Method for monitoring cancer cell number of cancer patient by adopting circulating DNA
  • Method for monitoring cancer cell number of cancer patient by adopting circulating DNA
  • Method for monitoring cancer cell number of cancer patient by adopting circulating DNA

Examples

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Embodiment Construction

[0041] The following examples will further illustrate the present invention, but do not thereby limit the present invention: 1. Take two patients as examples, and carry out detailed tracking of their therapeutic effects. The first case was a patient with breast cancer and the second case was a patient with non-invasive small cell lung cancer.

[0042] 1. Plasma Separation

[0043] 1) Blood is sucked into a sterile centrifuge tube;

[0044] 2) Immediately start centrifugation, 4°C, 1600g centrifugation for 10min;

[0045] 3) After centrifugation, suck the supernatant into a new centrifuge tube;

[0046] 4) Centrifuge at 16000g for 10min at 4°C;

[0047] 5) Aspirate the supernatant into a 2ml sterile centrifuge tube;

[0048] 6) Store at -20°C.

[0049] 2. ctDNA extraction

[0050] 1) Add 1ml plasma sample to 2ml EP tube, then add 1ml lysis buffer, 30μl proteinase K and 60μl nanoparticle adsorption magnetic beads. Fully invert and mix for 5min.

[0051] 2) Put the mixed ...

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Abstract

The invention provides a novel method for monitoring the cancer cell number of a cancer patient by adopting circulating DNA. According to the novel method, tumor DNA fragments in plasma of the cancer patient are separated and extracted by adopting a specific free small fragment DNA extraction kit; the obtained tumor DNA fragments are taken as a template, a tumor susceptible gene personalized molecular probe is designed for carrying out QPCR detection on the gene copy number, the detection periods of the tumor patients are different, range from 2 weeks to 1 month, and are determined according to the chemotherapy periods of patients. As the convenient, non-invasive, repeatable and highly-sensitive liquid biopsy technology, the detection method can be applied to various tumor susceptible genes and cancers, and belongs to an important assessment means for the cancer treatment effects, drug resistance and recurrent risk in advance of the clinical tumor marker detection.

Description

technical field [0001] The invention belongs to clinical auxiliary detection application technology, and in particular relates to a method for monitoring the number of tumor cells in cancer patients by using circulating DNA. Background technique [0002] Circulating tumor DNA (ctDNA) is a class of DNA fragments derived from tumor cells, with a size between 100-300 bp. It is endogenous DNA that is partially degraded after being released into the peripheral blood circulation by body cells. As early as 1947, Mandcl and Metals discovered free small fragments of DNA in the blood. In 1977, Leon et al. found that the level of free DNA in the blood circulation of tumor patients was significantly higher than that of normal people. Domirguez et al. detected plasma DNA and tumor tissue DNA in patients with bladder cancer, and found that the changes in 17 of 27 bladder cancer patients were very similar. Szymanska et al. detected plasma DNA and tumor DNA in 29 patients with liver cancer....

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2531/113C12Q2537/165
Inventor 程澎贾世哲
Owner 程澎
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