Detection of fluorescent-labeled MCF (Macrophage Chemotactic Factor) tumor marker based on biosensor

A biosensor and tumor marker technology, applied in the field of detection of fluorescently labeled MCF tumor markers based on biosensors, can solve problems such as blocking telomerase activity and unclear molecular mechanism of telomerase

Inactive Publication Date: 2014-10-08
QINGDAO UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

Telomerase inhibitors provide a new field for the treatment of tumors, but it is still difficult and important to select telomerase inhibitors that are highly selective for tumor cells and have no damage to normal cells
In addition, the regulatory mechanism of telomerase and the molecular mechanism of telomerase synthesis are still not very clear, and there are still many questions to be studied and discussed whether telomerase activity can be blocked from the level of telomerase protein and its gene.

Method used

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  • Detection of fluorescent-labeled MCF (Macrophage Chemotactic Factor) tumor marker based on biosensor
  • Detection of fluorescent-labeled MCF (Macrophage Chemotactic Factor) tumor marker based on biosensor
  • Detection of fluorescent-labeled MCF (Macrophage Chemotactic Factor) tumor marker based on biosensor

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Experimental program
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Embodiment 1

[0033] Detection of fluorescently labeled MCF tumor markers based on biosensors (see figure 1 )

[0034] 1. Preparation of biological probes

[0035] (1) Preparation of gold nanoparticles

[0036] The synthesis of gold nanoparticles is prepared by reducing tetrachloroauric acid with trisodium citrate. The specific preparation method is as follows:

[0037] 1% sodium citrate: Weigh 0.0306g of sodium citrate and dissolve in 3mL of water.

[0038] 0.01% chloroauric acid: Take 500 μL of 1% chloroauric acid, put it in a 50 mL volumetric flask, shake well, and set aside.

[0039] Assemble the distillation apparatus (a 250mL three-neck flask, spherical condenser, water inlet pipe and water outlet pipe), add 50mL of 0.01% chloroauric acid to it, adjust the rotor speed, and heat to boiling. Quickly add a certain amount of sodium citrate, and boil for 20 minutes. At this time, the solution in the flask gradually changed from colorless to wine red, and was naturally cooled to room ...

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Abstract

The invention discloses a method for detecting a tumor marker through a novel biochemical sensor which is constructed based on integration of a biological probe and DNA (Deoxyribose Nucleic Acid) circle amplification. The method comprises the following steps: carrying out connection modification on the surface of a magnetic ball through hairpin DNA and a precursor 1, adding telomerases for forming hairpin structures and further bonding with gold nanoparticles which are modified by a precursor 2 and marked with a large quantity of fluorescent molecules, and enhancing fluorescent signals through circle amplification; and finally, quantitatively analyzing telomerase in tumour cells by using the fluorescent signals so as to detect the tumor cells as soon as possible in medicine.

Description

technical field [0001] A biosensor usually refers to an analytical device that is tightly combined with a biosensitive component and a converter, and has a selective and reversible response to a specific type of chemical substance or biologically active substance. It is an indispensable advanced detection and monitoring method for the development of biotechnology, and it is also a method for rapid and microanalysis of substances at the molecular level. Background technique [0002] The main components of biosensors are receptors and transducers. The main working principle is that the substance to be measured enters the sensitive layer of the fixed biofilm through diffusion, and undergoes biological effects through molecular recognition. The information generated is light, heat, sound, etc. It is converted into a quantifiable and processable electrical signal by the corresponding signal converter, and then amplified and output by the secondary instrument, and the current valu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 丁彩凤李小倩张伟张书圣
Owner QINGDAO UNIV OF SCI & TECH
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