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A gastric cancer biomarker based on peripheral blood and its detection method and application

A biomarker and peripheral blood technology, applied in the field of gastric cancer biomarkers and their detection, can solve the problems of low sensitivity, poor specificity, unsuitable for screening work, etc., to improve the sensitivity and specificity, and improve the sensitivity and specificity Effect

Active Publication Date: 2021-01-26
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gastroscopic biopsy is invasive, and commonly used clinical serum tumor-related markers such as carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), carbohydrate antigen 224 (CA224), and carbohydrate antigen 724 (CA724) have low sensitivity , poor specificity and other shortcomings, not suitable for large-scale screening work

Method used

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  • A gastric cancer biomarker based on peripheral blood and its detection method and application
  • A gastric cancer biomarker based on peripheral blood and its detection method and application
  • A gastric cancer biomarker based on peripheral blood and its detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Collection and preservation of blood samples

[0053] Peripheral blood samples were collected from Jinling Hospital Affiliated to Nanjing University School of Medicine, including 100 patients with gastric cancer, 30 patients with benign gastric diseases, and 75 healthy subjects.

[0054] Take 0.5mL fresh EDTA-K2 anticoagulated blood, and immediately add 1.5-2mL RNALock blood RNA stabilizer (Tiangen Biochemical Technology (Beijing) Co., Ltd.).

[0055] Immediately cover the tube cap, mix up and down 8-10 times, and place at room temperature for more than 2 hours. The mixed samples were stored in a -20°C low temperature refrigerator.

Embodiment 2

[0056] Embodiment 2: RNA extraction in blood sample

[0057] Step 1: When purifying a blood sample stored in a blood RNA stabilizer, first place the sample at room temperature or warm it up to room temperature in a 37°C water bath. Then centrifuge at 6600 rpm for 10 min, discard the supernatant with a pipette, and extract total RNA in the blood with the RNAprep Pure Blood Total RNA Extraction Kit (Tiangen Biochemical Technology (Beijing) Co., Ltd.). For specific operations, refer to the kit instructions.

[0058] Nanodrop2000 detects RNA concentration and purity, and performs subsequent operations.

Embodiment 3

[0059] Example 3: RNA m 6 Quantitative detection of A methylation

[0060] Using EpiQuik from Epigentek TM m 6 A RNA Methylation Quantification Kit (Colorimetric) kit, please refer to the kit manual for specific operation.

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Abstract

The invention discloses a gastric cancer biomarker based on peripheral blood, a detection method and application thereof. The invention also discloses the detection of peripheral blood RNA m 6 A reagent or kit for diagnosing gastric cancer. The specific peripheral blood RNA m provided by the present invention 6 A. The intermediate results of gastric cancer diagnosis can be used alone or in combination with related mRNA or serum tumor marker detection, which will help physicians to diagnose gastric cancer in combination with clinical symptoms, medical history or other examination information.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a gastric cancer biomarker based on peripheral blood and a detection method and application thereof. Background technique [0002] Gastric cancer, as a malignant tumor originating from the gastric mucosal epithelium, is one of the most common malignant tumors of the digestive system. In 2018, the number of new cases of gastric cancer worldwide is expected to exceed 1 million, and the number of deaths is about 783,000, ranking fifth in the incidence of malignant tumors and third in the fatality rate. More than 70% of new gastric cancer cases come from developing countries, and nearly half of the cases come from East Asia. Therefore, early diagnosis of gastric cancer is particularly important. At present, gastroscopic biopsy and detection of serum tumor-related markers are the main diagnostic tools. However, gastroscopic biopsy is invasive, and commonly used clinical ser...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 汪俊军王红胜葛李晨
Owner NANJING UNIV
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