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A white luminescent up-conversion nanoparticle and a test strip based on it to simultaneously realize the detection of multi-component tumor markers

A tumor marker, nanoparticle technology

Active Publication Date: 2020-10-23
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, when the existing up-conversion test strips detect multiple tumor markers at the same time, different T-lines need to use different up-conversion nanoparticles, the production process is complicated, and the detection efficiency is seriously affected.

Method used

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  • A white luminescent up-conversion nanoparticle and a test strip based on it to simultaneously realize the detection of multi-component tumor markers
  • A white luminescent up-conversion nanoparticle and a test strip based on it to simultaneously realize the detection of multi-component tumor markers
  • A white luminescent up-conversion nanoparticle and a test strip based on it to simultaneously realize the detection of multi-component tumor markers

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Embodiment 1

[0050] The detection of embodiment 1, CEA, AFP tumor marker

[0051] In this embodiment, CEA and AFP are used as tumor markers to be detected as examples, and the details are as follows.

[0052] 1. Preparation of UCNPs-antibody

[0053] 1.1. White luminescent up-conversion nanoparticles with a three-layer core-shell structure were prepared by the seed crystal method, which was based on NaGdF doped with Yb, Tm and Er 4 The nanoparticle is the inner core, and the outer core is wrapped with Eu-doped NaGdF 4 The first shell, and the first shell is wrapped with NaYF 4 The second shell, which constitutes Yb, Tm, Er and Eu co-doped NaGdF 4 :Yb / Tm / Er@NaGdF 4 :Eu@NaYF 4 Core-shell structure; specific preparation steps are as follows:

[0054] a. Synthesis of NaGdF doped with Yb, Tm and Er 4 Nanoparticles as the core

[0055] Weigh 10mL of oleic acid (OA), 10mL of octadecene (ODE), Gd(OAc) 3 0.0841g, Yb(OAc) 3 Add 0.0858g, 25μL of 0.1mol / L Tm aqueous solution, 2μL of 0.2mol...

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Abstract

The invention discloses a white light-emitting up-conversion nanoparticle and a test paper strip based on the white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers. According to the present invention, the nanoparticle has a three-layer core-shell structure, and emits white light under the excitation of near infrared light; and after the antibodies of multi-component tumor markers to be detected are labeled with the nanoparticle, the obtained conjugates are immobilized on the labeling complex binding zone of the test paper strip, such that the multiple tumor markers can be simultaneously detected; and with the nanoparticle and the test paper strip, the sensitive detection on the multiple tumor markers by the single up-conversion nanoparticle is achieved, and the advantages of simple operation, good specificity, high qualitative and quantitative real-time detection and high sensitivity are provided.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis, and in particular relates to an up-conversion test paper detection method for multi-component tumor markers. Background technique [0002] Malignant tumors are often referred to as cancer. The expression of some genes in the body is out of control. The malignant proliferation of cells destroys the normal life activities of the body. Its growth speed is fast, which will cause weight loss, fever, and serious organ damage. The final crisis life. At present, cancer has become the number one killer threatening people's lives. Therefore, early detection of cancer and timely clinical monitoring have become a major issue in modern biomedicine. With the maturity of nanotechnology, the combination platform of nanotechnology and cancer markers for the detection of tumor markers has become a hot spot. Colloidal gold test strips are the earliest widely used immunochromatographic test strips. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/63
CPCG01N21/63
Inventor 邓胜松高梦萍梅青松
Owner HEFEI UNIV OF TECH
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