A kind of detection method of procymidone in vegetables

A detection method, the technology of procymidone, is applied in the field of analytical chemistry, which can solve the problems of low detection limit and inapplicability, and achieve the effect of simple detection method, reduced use and depreciation, and wide application range.

Active Publication Date: 2021-09-21
WUXI ZODOLABS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The substance to be tested was extracted with acetonitrile, homogenized at a high speed on a homogenizer, filtered, 5-7g of sodium chloride was salted out, 10.0mL of the filtrate was extracted and dried, dissolved in n-hexane, rinsed with Florisil column, and collected The lotion was dried to a constant volume and then analyzed by gas chromatography to realize the determination of procymidone residues; this method has the advantages of high sensitivity and low detection limit, but as a relatively expensive instrument, gas chromatography is not applicable in industrial production Screening of a large number of samples

Method used

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  • A kind of detection method of procymidone in vegetables
  • A kind of detection method of procymidone in vegetables

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Experimental program
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Effect test

Embodiment 1

[0076] (1) Weigh 100 parts of leeks with a mass of 2 g and crush them into a 10 mL centrifuge tube, add them into a mixture of 4 mL of acetonitrile and 1 mL of saturated sodium chloride solution, vortex for 2 min, and vortex After the end, centrifuge at a rate of 4000r / min for 1min. After centrifugation, take the supernatant into another 10mL centrifuge tube, add 1mL saturated sodium chloride solution to shake for 0.5min, and centrifuge at a rate of 4000r / min for 1min for cleaning. , to obtain the extract;

[0077] (2) adding graphitized carbon black with a mass of 0.1 g to the extract obtained in step (1) and mixing, centrifuging for 1 min at a rate of 4000 r / min for decolorization to obtain a decolorized solution;

[0078] (3) Put the decolorized solution obtained in step (2) into another 10mL centrifuge tube, first add 1mL of sodium hydroxide solution with a concentration of 0.1mol / L, and open the ring at a temperature of 100°C Reaction, the reaction time is 5min, nitrogen...

Embodiment 2

[0085] (1) Weigh 100 parts of cucumbers, each with a mass of 1g, and crush them into a 10mL centrifuge tube, add them into a mixture of 2mL acetonitrile and 1.5mL saturated potassium chloride solution, vortex for 2min, and vortex After spinning, centrifuge at a speed of 3000r / min for 3min. After centrifugation, take the supernatant into another 10mL centrifuge tube, add 1mL saturated potassium chloride solution to shake for 1min, and centrifuge at a speed of 3000r / min for 3min for cleaning. , to obtain the extract;

[0086] (2) Adding graphitized carbon black with a mass of 0.05 g to the extract obtained in step (1) and mixing, centrifuging for 3 min at a speed of 3000 r / min for decolorization to obtain a decolorized solution;

[0087] (3) Put the decolorized solution obtained in step (2) into another 10mL centrifuge tube, first add 1.5mL of potassium hydroxide with a concentration of 0.05mol / L, and open the ring at a temperature of 90°C Reaction, reaction time 8min, nitrogen...

Embodiment 3

[0092] (1) Weigh 100 copies of the eggplant to be tested with a mass of 3g and crush it into a 10mL centrifuge tube, add it to a mixture of 6mL acetonitrile and 0.5mL saturated potassium chloride solution, vortex for 3min, vortex After spinning, centrifuge at a speed of 6000r / min for 1min. After centrifugation, take the supernatant into another 10mL centrifuge tube, add 1mL saturated potassium chloride solution to shake for 0.5min, and centrifuge at a speed of 6000r / min for 1min. cleaning to obtain the extract;

[0093] (2) adding graphitized carbon black with a quality of 0.15 g to the extract obtained in step (1) and mixing, centrifuging for 1 min at a speed of 6000 r / min for decolorization to obtain a decolorized solution;

[0094] (3) Put the decolorized solution obtained in step (2) into another 10mL centrifuge tube, first add 0.5mL of sodium hydroxide with a concentration of 0.15mol / L, and open the ring at a temperature of 120°C Reaction, the reaction time is 3min, nitr...

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Abstract

The invention provides a detection method for procymidone in vegetables, the detection method comprising the following steps: (1) extracting the substance to be tested to obtain an extract; (2) adding the extract obtained in step (1) The decolorizing agent is decolorized to obtain a decolorized solution; (3) the decolorized solution obtained in step (2) undergoes a ring-opening reaction under the action of an alkali to obtain a ring-opened compound, and then the ring-opened compound is condensed under the action of hydroxylamine reaction to obtain hydroxamic acid compounds; (4) acidify the hydroxamic acid compounds obtained in step (3), add buffer solution to adjust pH, then add color developing agent for color development, and detect by visual colorimetry Procymidone in vegetables; the detection method has the advantages of simple detection method, short analysis period, low cost, wide application range, good data accuracy, can be used for the detection of procymidone, and meets the production requirements of enterprises.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a method for detecting procymidone in vegetables. Background technique [0002] Procymidone is a systemic fungicide with protective and therapeutic effects. It is used for vegetable crops such as rapeseed, radish, eggplant, and leek to prevent gray mold and sclerotinia. The effect is obvious under low temperature and high humidity conditions. According to GB2763-2014 "National Food Safety Standard Maximum Residue Limits in Foods", the maximum residue limit of procymidone in leek is 0.2mg / kg. According to the survey, the main reason for the excessive residue of procymidone in chives is that the growers use this agent to prevent the occurrence of Botrytis cinerea. Chive gray mold is a disease that infects many times in one growing season. It mainly damages the leaves of leek. Typical symptoms include white spots, dry tips and wet diseases. A small amount of pesticide residues wil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N1/28
CPCG01N1/28G01N21/78
Inventor 张文涛任水英周丽邹晓兰朱华玲李林
Owner WUXI ZODOLABS BIOTECH
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