Micro RNA (miRNA) biomarker combination and kit used for diagnosing or assisting in diagnosing gastric cancer

A technology for biomarkers and auxiliary diagnosis, which is applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc., can solve problems such as missed diagnosis, and achieve the effect of excellent accuracy and sensitivity

Pending Publication Date: 2020-06-23
陈锦飞 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although gastric cancer is diagnosed clinically through gastroscopy and biopsy pathology, it is an invasive operation, and the diagnosis result has a lot to do with the experience of equipment, operating doctors and pathologists, and missed diagnosis may occur

Method used

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  • Micro RNA (miRNA) biomarker combination and kit used for diagnosing or assisting in diagnosing gastric cancer
  • Micro RNA (miRNA) biomarker combination and kit used for diagnosing or assisting in diagnosing gastric cancer
  • Micro RNA (miRNA) biomarker combination and kit used for diagnosing or assisting in diagnosing gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Eleven significantly up-regulated miRNAs in gastric cancer tissues were screened out through the TCGA high-throughput miRNA expression database (n=477), including miR-21, miR-196a-1, miR-146b, miR-196b, miR-135b, miR -181b-1, miR-181a-1miR-93, miR-335, miR-196a-2 and miR-18a, 10 miRNAs after cross-platform verification, miR-21, miR-196a, miR-146b, miR -196b, miR-135b, miR-181b-1, miR-181a-1miR-93, miR-335 and miR-18a, wherein miR-196a-1 and miR-196a-2 are the same sequence (miR-196a) , please refer to Table 1 for the specific sequence.

[0060] Table 1 Sequences of miRNA biomarker combinations

[0061]

[0062] The prediction effects of the above 10 miRNAs were verified on two independent high-throughput data sets (the screening characteristics of the data sets refer to Table 5). For the results, please refer to the attached figure 1 , the area under the ROC curve AUC obtained was greater than 0.93.

[0063] Based on the relationship between the expression of miRN...

Embodiment 2

[0068] The miRNA biomarker combination provided in Example 1 was used for screening of early gastric cancer.

[0069] 1. Serum Sample Collection

[0070] Whole blood was collected in Sarstedt with coagulant Serum-Gel 9ml tubes (cat.no.02.1388) (or other collection tubes without coagulant or EDTA). For complete coagulation, the test tube containing the coagulation accelerator needs to be placed at room temperature (15-25°C) for 10 minutes to 1 hour. It should be noted that the test tube containing the coagulant needs to be placed for 10 minutes, and the test tube without the coagulant needs to be placed for at least 30 minutes;

[0071] Centrifuge the test tube at 1900×g (3000rpm), 4°C for 10 min; transfer the supernatant serum (yellow) to a new test tube with a conical bottom (usually 10 ml of whole blood can obtain 3-5 ml of serum); put the conical bottom The plasma in the test tube was centrifuged at 16,000×g at 4°C for 10 minutes; the supernatant was transferred to a ne...

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Abstract

The invention discloses a micro RNA (miRNA) biomarker combination and kit for diagnosing or assisting in diagnosing gastric cancer, and relates to the technical field of gastric cancer detection. ThemiRNA biomarker combination comprises miR-18a, miR-93, miR-146b, miR-181a, miR-181b, and miR-335, provides a new way for screening of early gastric cancer, and is superior to an existing tumor markerin the aspects of accuracy and sensitivity of detection.

Description

technical field [0001] The present invention relates to the technical field of gastric cancer detection, in particular to a miRNA biomarker combination and a kit thereof for diagnosis or auxiliary diagnosis of gastric cancer. Background technique [0002] Gastric cancer is currently the fifth most common malignancy and the third leading cause of malignancy-related death in the world. Although treatment methods including surgery, chemotherapy, and radiotherapy have been continuously improved in recent years, the prognosis of patients with advanced gastric cancer is still poor. However, early gastric cancer is often difficult to be found due to its hidden symptoms, which leads to missing the best treatment time. [0003] At present, CEA, CA19-9, CA72-4, CA125, CA24-2, CA50 and AFP are mostly used clinically for the diagnosis and detection of gastric cancer, but their accuracy needs to be improved. Although gastric cancer is diagnosed clinically through gastroscopy and biopsy...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178C12Q2600/166
Inventor 陈锦飞高峰陈玥彤霍新颖薛毅琪
Owner 陈锦飞
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