31 results about "Sophoricoside" patented technology

The invention discloses a novel technology for extracting genistein by using sophoricoside as a raw material, comprising the following steps of: crushing sophoricoside, heating by water and carrying out reflux extraction; concentrating a water decoction to an extract, followed by reflux and hydrolysis; cooling and filtering to obtain a crude product; carrying out recrystallization on the crude product to obtain the genistein with the purity being over 98%. The invention has the following advantages: the price of raw materials is low, the product output and high purity are high, the production cost is low, the technology is simple and easy to operate, the requirement on equipment is low and reagents are easy to recover.

The invention relates to a medicine that is a mongolian medicine for treating hemorrhoids. The medicine is made from raw ingredient medicines by weight parts as follows: sophoricoside is 30 to 35 parts, garden burnet is 15 to 20 parts, bitter orange is 15 to 20 parts, scutellaria is 15 to 20 parts, catnip is 15 to 20 parts, coptis is 15 to 20 parts, biota orientalis is 15 to 20 parts, radix saposhinikoviae is 8 to 12 parts, dangguiwei is 12 to 18 parts, and Gentiana macrophylla is 15 to 25 parts. The mongolian medicine which is made by adopting the raw ingredient medicines has the efficacies of detumescence as well as blood stasis removing, regeneration as well as hemostasis, and heat clearing as well as acesodyne; a large number of experiments and clinical application prove that the mongolian medicine has unique therapeutic effect on various types of the hemorrhoids and has the advantages that the course of treatment is short, the cost is low, no toxic and side effects exist, etc., and the application prospect is very considerable.

The invention relates to application of sophoricoside in the preparation of a drug for preventing and / or remedying postmenopausal female articular cartilage degeneration or osteoarthritis. The sophoricoside has obvious curative effect, does not have side effects on uterus, breast and the like caused by animal estrogen, is convenient for extracting and has abundant sources and wide market prospect.

The invention relates to a rodenticide resisting breeding of female rats by taking sophoricoside as an active ingredient, belonging to the technical fields of biological prevention and control. The damage caused by rats is always a serious disaster problem in the world, which causes damage to agriculture, forestry and animal husbandry, seriously damages ecological environment and threatens human health. Through the years, people use multiple methods to control the population quantity of rats, and a method for controlling the population quantity of the rats by a breeding-resisting technique is a new technique developed in recent years. The invention relates to the rodenticide resisting breeding of female rats by taking sophoricoside as an active ingredient, which is used for controlling the murine reproduction. Compared with a traditional ecological deratization method and chemical poison bait method, the breeding resisting method has long effective time, obvious control effect, small toxic and side effects on the environment and the like, and can more effectively inhibit the super compensatory effect of rat population. If the rodenticide resisting breeding of female rats provided by the invention can be applied to the production of agriculture, forestry and animal husbandry, the rat population can be effectively controlled and the wide economic benefit can be achieved.

The invention relates to an Aspergillus aculeatus ZJ bacterial strain and an application thereof. The collection number of the Aspergillus aculeatus ZJ is CCTCC NO.M2011264. The bacterial strain is mainly used for converting effective components of the pod of Chinese scholartree to produce 5,7,8,4'-tetrahydroxyisoflavone. The specific method provided by the invention comprises the following steps of: acting the Aspergillus aculeatus ZJ with strong specificity or the produced enzyme on the pod of Chinese scholartree or extracting solution thereof; converting glucosides compounds including sophoricoside and the like, genistein and the like to produce the 5,7,8,4'-tetrahydroxyisoflavone. The invention provides the method for producing the 5,7,8,4'-tetrahydroxyisoflavone through utilizing the Aspergillus aculeatus with high specificity and high conversion efficiency to convert the sophoricoside; and the method provided by the invention has moderate conditions, green process and simple requirements on the process and the device, and has a very good application value.

The invention relates to a herbal drug for hemorrhoids with natural plants, in particular to a drug with specific formulation, comprising a plurality of active pharmaceutical ingredients, certain amount of penetration enhancer and certain amount of pharmacyeutical adjuvant with the following weight proportion. The active pharmaceutical ingredient comprises one hundred shares of sophoricoside, eighty shares of panax notoginseng, one hundred and ten shares of lightyellow sophora root, one hundred shares of bletilla striata, one hundred shares of common cnidium fruit, one hundred shares of patrinia scabiosaefolia link, sixty-five shares of moxa leaf, eighty shares of pursslane, sixty-five shares of honeysuckle, sixty-five shares of parsnip, fifty shares of alumen, fifty shares of borax, fiveshares of borneol, and thirty shares of licorice. The penetration enhancer comprises a certain amount of laurocapram and a certain amount of propanediol with the weight proportion of eighty to fifteen - thirty; the adding amount of the penetration enhancer is point zero eight to point four percent of the weight of the active pharmaceutical ingredient. The drug has the advantage of being more effective than oral drugs and external lotion due to full play of effective ingredients by adding penetration enhancer in specific prescription and formulation.

The invention relates to an extraction method of genistein. The method comprises the following steps: fully mixing the grinded raw material pagodatree pod with the grain size of 20-80 meshes and 80-95v% ethanol in the ratio of 1 / 8-1 / 15(w / v), pumping into a hydrodynamic force sonic energy generator, introducing sophoricoside to extraction solvent under the action of acoustic wave with the frequency of 1k-3kHz and the sound pressure level of 216-236dB which is generated by the flowing of the hydrodynamic force sonic energy generator, while performing further hydrolysis; after the solid-liquid separation, using ethanol to dissolve water-insoluble precipitate which is obtained by removing solvent in liquid part, after the ethanol dissolving process, performing silica gel column chromatography, using ethanol for elution, collecting the eluant, and removing solvent to obtain genistein. The method is a green extraction method without additional energy under the action of hydrodynamic force sound energy, the genistein can be obtained directly, the extraction process can be completed without any additional auxiliary component at room temperature, high purity extract can be obtained, and the damage of the effective ingredient can be avoided mostly. The treatment process is simple, the equipment is stable and reliable, the extraction equipment and the floor space are greatly reduced, andthe continuous operation can be realized, the unit capacity is high, the extraction time is greatly reduced, and the extraction cost is only 1 / 10-1 / 20 of that of the conventional method.

The invention discloses a method for producing long-chain glycosylated sophoridoside, which belongs to the technical field of enzyme engineering and fermentation engineering. The invention provides a method for producing long-chain glycosylated sophoridosides, in which cyclodextrin glucosyltransferase mutants S77N, Y195S, Y195I, S77N, S77N / Y195S and S77N / Y195I are used, so that the long-chain The content of chain glycosylated sophoric acid was significantly increased. By optimizing the reaction conditions, the content of long-chain glycosylated sophoroside in the reaction solution was increased to 9.8g / L, and the long-chain glycosylated sophoric acid in the reaction solution was reduced to 9.8g / L. The ratio of the glycoside content to the total glycosylated sophoridoside content in the reaction solution is increased to 45%.

The invention discloses a kind of Rhizopus oryzae LJH3 and its application in the preparation of genistein by biotransforming sophoraside. The application is the fermented liquid obtained by fermenting and cultivating Rhizopus oryzae LJH3, or the fermented liquid is filtered to remove bacteria. The mixture of the filtrate of the body and the equal volume of distilled water is used as a biocatalyst, and the transformation system is formed with sophorin as the substrate. The transformation reaction is carried out at 30-38°C and 200-250r / min constant temperature oscillation. After the transformation reaction is completed, The conversion liquid is separated and purified to obtain genistein; in the present invention, when the substrate feeding concentration is 1 g / L, the conversion yield of genistein is 84.2%, and the purity is 92.5%. The invention converts low-activity sophorin into genistein with better activity, which is an optional production method for producing genistein. The process has the advantages of low production cost, high conversion rate, and small environmental pollution.

The invention discloses a method for extracting flavone ingredients and extracting kaempferide from sophoricoside and relates to the medicine. The method comprises the following steps: de-ionized water is added into the raw material of the sophoricoside, and reflux extracting is carried out in heating condition; extracting liquid-purpose diatomite and filtering is carried out; the filter solutionis put on a macroporous resin column, impurity is washed by water and the filter solution is eluted by high-concentration ethanol; concentrating and drying is carried out on the eluting solution, thus obtaining the sophoricoside flavone extract; hydrolysate is added into the sophoricoside flavone extract and hydrolyzing is carried out in heating condition; pH value is adjusted to neutral after hydrolysis; steaming and removing alcohol, filtering, washing repeatedly by water are carried out on filter residue; drying and extracting are carried out on the filter residue by an extracting agent ofmixed solution of aether and ethanol, the extract solution is steamed and the high-purity kaempferide is obtained. The method can be used for producing high-purity kaempferide flavone extract and kaempferide and has the advantages of simple process, good product quality, high degree of comprehensive utilization, being suitable for industrial production and low cost.

The invention relates to a drug for internal hemorrhoid, external hemorrhoid and mixed hemorrhoid treatment, with therapeutic effect to hemorrhoid bleeding, belonging to the field of Chinese patent medicine, comprising cordate houttuyia, purslane, liquirice, angelica, angelica sinensis, gentiana, astragalus, burnet, sophoricoside, plaster, Chinese rhubarb and Chinese honeylocust (or Chinese locust abnormal fruit), which is characterized that the drug can radically curing internal hemorrhoid, external hemorrhoid and mixed hermorrhoid; and the therapeutic effect greatly exceeds prior hemorrhoids drugs on the market according to a research on Chinese herbal medicine market for over thirty years. The drug has the advantages of world advanced radical therapeutic effect for hemorrhoids, safety and reliability, adequacy of drug resource, low cost and simple manufacturing technique, hopefully to be recognized soon.

The invention relates to ointment preparation for treating hemorrhoid, which is prepared by using natural plant as the main pharmaceutic ingredients and using special medicine as the auxiliary material. The ointment is prepared from the following materials in parts by weight: 80 parts of pseudo-ginseng, 80 parts of purslane, 110 parts of lightyellow sophora root, 100 parts of rhizoma bletillae, 100 parts of fructus cnidii, 100 parts of patrinia, 65 parts of folium artemisiae argyi, 100 parts of sophora fruit, 65 parts of honeysuckle, 65 parts of radices silerism, 50 parts of alum, 50 parts ofborax, 5 parts of borneol, 30 parts of liquorice, 20-50 parts of stearic acid, 20-50 parts of polyethylene glycol-6000, 500-900 parts of polyethylene glycol-400, 0.8-4.8 parts of laurocapram and 0.2-1.2 parts of propylene glycol. As special pharmaceutic adjuvant is added in the ointment, the invention can effectively prevent matrine in the medicine from changing into oxymatrine, enhance the functions of antiphlogosis and abirritation, improve the release and penetration ability of sophoricoside and fully exert the pharmacologicalaction of detumescence, antivirus, hemostasis, and the like of sophoricoside.

The invention relates to a medicine that is a mongolian medicine for treating hemorrhoids. The medicine is made from raw ingredient medicines by weight parts as follows: sophoricoside is 30 to 35 parts, garden burnet is 15 to 20 parts, bitter orange is 15 to 20 parts, scutellaria is 15 to 20 parts, catnip is 15 to 20 parts, coptis is 15 to 20 parts, biota orientalis is 15 to 20 parts, radix saposhinikoviae is 8 to 12 parts, dangguiwei is 12 to 18 parts, and Gentiana macrophylla is 15 to 25 parts. The mongolian medicine which is made by adopting the raw ingredient medicines has the efficacies of detumescence as well as blood stasis removing, regeneration as well as hemostasis, and heat clearing as well as acesodyne; a large number of experiments and clinical application prove that the mongolian medicine has unique therapeutic effect on various types of the hemorrhoids and has the advantages that the course of treatment is short, the cost is low, no toxic and side effects exist, etc., and the application prospect is very considerable.

The invention belongs to the technical field of plant extraction, and discloses a process for extracting sophoraside from Sophora japonica, comprising the following steps: crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→washing Desorption → evaporation → drying → dissolution → crystallization. The process of the invention is simple and feasible, and the extraction rate and purity of the total flavonoids and sophosides are high.