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Process for extracting sophorin from Sophora japonica and its use in anti-inflammation

A technology of sophorin and sophora, which is applied in anti-inflammatory agents, non-central analgesics, organic chemistry, etc., can solve the problems of large enzyme dosage and low extraction rate, and achieve improved extraction rate and purity, and high dissolution rate , high extraction efficiency and purity

Active Publication Date: 2021-07-23
刘学键
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many techniques for extracting Sophora flavonoids, most of which mainly use chemical reagents for extraction, while conventional extraction methods have unsafe factors such as organic solvent residues, which pose a potential threat to human health. Improvements have been made, such as "Process optimization of enzymatic extraction of total flavonoids in Sophora japonicus, Food Technology Science 2012" used enzymatic method to extract flavonoids from Sophora japonicus, which has the advantages of non-toxicity and mild reaction conditions, but this method has the advantages of enzyme dosage Larger, lower extraction rate and other defects, it needs to be further improved

Method used

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  • Process for extracting sophorin from Sophora japonica and its use in anti-inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The technology of extracting sophorin from Sophora japonica, it comprises the following steps:

[0028] Crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→elution→evaporation→drying→dissolving→crystallization.

[0029] Specifically include:

[0030] Take Sophora japonicus, pulverize, and pass through a 100-mesh sieve to obtain Sophora japonicus powder, then place it in a reactor, control the pressure in the reactor to 3MPa, hold the pressure for 30s, and perform pulse electric field treatment. The field strength of the electric field is 10kv / cm, and the total treatment The time is 200 μs, the pulse width is 4 μs, and the pulse frequency is 400pps; then add 5 times the weight of water, use a high-speed shearing machine to cut for 3 minutes, and the shear speed is 5000rpm, and then add according to the ratio of 20,000 U:100g Sophora japonicus powder For cellulase, control the enzyme reaction...

Embodiment 2

[0032] The technology of extracting sophorin from Sophora japonica, it comprises the following steps:

[0033] Crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→elution→evaporation→drying→dissolving→crystallization.

[0034] Specifically include:

[0035] Take Sophora japonicus, pulverize, pass through a 200-mesh sieve to obtain Sophora japonicus powder, then place it in a reactor, control the pressure in the reactor to 2MPa, hold the pressure for 40s, and perform pulse electric field treatment. The field strength of the electric field is 10kv / cm, and the total treatment The time is 200 μs, the pulse width is 4 μs, and the pulse frequency is 400pps; then add 4 times the weight of water, use a high-speed shearing machine to cut for 3 minutes, and the shear speed is 5000rpm, and then add according to the ratio of 10,000 U:100g Sophora japonicus powder For cellulase, control the enzyme reaction tem...

Embodiment 3

[0049] Determination of total flavonoids: Taking Huaijiao in southern Shandong as an example, the experiment of the present invention uses 1000g Huaijiao, and the content of total flavonoids in Huaijiao powder measured by HPLC method is 19.7%, and the experimental groups are respectively selected from Example 1 and Comparative Example 1- 4. Obtain the weight of total flavonoids extract by weighing, measure the total flavonoids content in the extract by HPLC method, calculate the yield of total flavones, calculation formula: yield=((quality × content of extract) / (sophora japonicus quality × Content)) × 100%. The specific results are shown in Table 1:

[0050] Table 1

[0051] group Sophora japonicus powder weight (g) Total flavonoid extract (g) Total flavonoid content in extract % Yield% Example 1 1000 245.9 75.8 94.6 Comparative example 1 1000 234.7 70.4 83.9 Comparative example 2 1000 221.8 68.1 76.7 Comparative example 3 1...

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Abstract

The invention belongs to the technical field of plant extraction, and discloses a process for extracting sophoraside from Sophora japonica, comprising the following steps: crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→washing Desorption → evaporation → drying → dissolution → crystallization. The process of the invention is simple and feasible, and the extraction rate and purity of the total flavonoids and sophosides are high.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a process for extracting sophorin from Sophora japonica and its application in anti-inflammation. Background technique [0002] Sophora japonica L. is the fruit of Sophora japonica L., a leguminous plant. It is a commonly used traditional Chinese medicinal material recorded in the Pharmacopoeia. The medicinal Sophora japonica L. should usually be harvested in winter. Ripe pods. The dried Sophora japonicus pods picked after the winter solstice are cylindrical, sometimes curved, and the seeds are constricted into beads. After the foreskin, they are brown or yellowish brown, and the seeds are brownish black; Chinese medicine theory believes that Sophora japonicus is cold in nature and has a strong taste. Bitter, returns to the liver and large intestine meridian, has the functions of clearing away heat and purging fire, cooling blood and stopping bleeding. It is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/26C07H1/08A61P29/00
CPCA61P29/00C07H1/08C07H15/26
Inventor 孙波王芳王洲
Owner 刘学键
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