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Quality evaluation method of sophora japonica-scutellaria baicalensis ointment

A technology for quality evaluation and ointment, applied in the field of quality evaluation of Huaiqin ointment, can solve the problems of high price of reference substance and low supply, and achieve the effect of low price and cost reduction

Pending Publication Date: 2020-10-09
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem that the existing quality standard of Huaiqin ointment is relatively simple in the prior art, and the reference substance is expensive and has little supply when measuring multiple components, the invention provides a quality evaluation method for Huaiqin ointment. Methods Combining fingerprint similarity analysis and quantitative analysis, the method is accurate, efficient, repeatable, stable, and characteristic, and can be used for quality control of Huaiqin Ointment

Method used

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Experimental program
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Effect test

Embodiment 1

[0032] (1) Chromatographic conditions

[0033] Column: Agilent ExtendC 18 (250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile (A) -0.2% phosphoric acid solution (B), gradient elution (0~10min, 13%A; 10~40 min, 13%A→16% A; 50~60 min, 40%A → 50%A); flow rate: 1.0mL min -1 , detection wavelength: 230 nm, column temperature: 25°C, injection volume 10 μL.

[0034] (2) Preparation of mixed reference solution

[0035]Accurately weigh the appropriate amount of paeoniflorin, rutin, genistin, sophorin, naringin, neohesperidin, baicalin, wogonin, and paeonol reference substances, put them in a 100 mL measuring bottle, add methanol Dissolve and dilute to the mark, shake well to obtain mass concentrations of 0.1688, 0.0986, 0.0958, 0.0876, 0.1058, 0.0687, 0.1444, 0.0961, 0.1450 mg·mL -1 mixed solution.

[0036] (3) Preparation of the test solution

[0037] Take an appropriate amount of Huaiqin ointment and mix evenly, take about 1g, weigh it accurately, put it in a stoppered Erlenmeyer...

Embodiment 2

[0057] Multicomponent Quality Control

[0058] (1) Establishment of relative content calculation method

[0059] Since the maximum absorption wavelengths of each compound are different, based on the overall characteristics of the fingerprints, in order to achieve high-sensitivity determination of the content of multiple index components and save the use of reference substances, the maximum absorption wavelength of each group of index components is selected at a low price and easy to obtain. , Sophorin, paeoniflorin and baicalin are used as reference substances with good stability, and their relative contents are determined. Based on the DAD absorption spectrum of 21 peaks, see Figure 4 , divided into A, B, C 3 groups, the maximum absorption wavelength of peak 1, 2, 5, 6, 7, 8, 10, 11, 12 in group A is 260nm. Paeoniflorin (concentration 0.0876mg / ml) was used as reference for calculation; the maximum absorption wavelength of peaks 3, 4, 9, and 19 in group B was 230nm, and pea...

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Abstract

The invention discloses a quality evaluation method of sophora japonica-scutellaria baicalensis ointment. The method is characterized in that a method of combining fingerprint spectrum similarity analysis and index component relative content analysis is adopted; when the similarity between the fingerprint spectrum of the to-be-detected sophora japonica-scutellaria baicalensis ointment and the contrast fingerprint spectrum of the sophora japonica-scutellaria baicalensis ointment is higher than 0.95, index component relative content analysis is carried out, and an HPLC-DAD method is adopted forfingerprint spectrum similarity analysis and index component relative content analysis of the sophora japonica-scutellaria baicalensis ointment. The method is verified by methods such as precision, stability, repeatability and the like, is accurate and sensitive, compares whether 21 common peaks exist in the fingerprint spectrum or not, has a large amount of information, can effectively evaluate the quality of the sophora japonica-scutellaria baicalensis ointment, and can realize qualitative analysis. The sophoricoside, the paeoniflorin and the baicalin are adopted to realize synchronous monitoring of 21 components, so that the method is more efficient and convenient; particularly, the relative content control of 21 index components of the sophora japonica-scutellaria baicalensis ointmentcan be realized under the condition of lack of reference substances; and the cost of multi-component content determination can be effectively reduced.

Description

technical field [0001] The invention belongs to the field of analysis and evaluation of traditional Chinese medicines, in particular to a quality evaluation method of Huaiqin ointment. Background technique [0002] Huaiqin Ointment is composed of Huaijiao (stir-fried), Radix Paeoniae Rubra, Cortex Moutan, Angelica, Fangfeng, Fructus Citrus Citrifolia, Scutellaria baicalensis and eight medicinal herbs of Radix Aconitum. Damp-heat lower injection syndrome, symptoms include blood in the stool, falling pain, local discomfort, prolapse of hemorrhoids. In the prescription, Huaijiao has the effects of clearing heat and detoxifying, cooling blood and stopping bleeding, and is a monarch drug; Scutellaria baicalensis has the effect of clearing heat, drying damp and detoxifying, Moutan cortex clears heat and cools blood, and increases the power of monarch medicine to clear heat and cool blood, and is a minister drug; Angelica nourishes blood and activates blood, Radix Paeoniae Rubra c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686G01N30/8679
Inventor 许丽丽林永强刘群栾永福臧远芳刘洪超徐兴燕于雅萌高登璐何军
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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