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Detection method for fingerprint spectrum of mountain ginseng in forest

A technology of fingerprint spectrum and detection method, which is applied in the detection field of fingerprint spectrum

Inactive Publication Date: 2019-07-09
SPH XING LING SCI & TECH PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current identification methods of ginseng age mainly rely on the identification of traits and the experience of veteran pharmacists and farmers, which inevitably has a certain degree of subjectivity. With the diversification of commodity processing, the limitations of trait identification become more and more obvious

Method used

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  • Detection method for fingerprint spectrum of mountain ginseng in forest
  • Detection method for fingerprint spectrum of mountain ginseng in forest
  • Detection method for fingerprint spectrum of mountain ginseng in forest

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1. Sample pretreatment

[0068] Preparation of the test solution: Grind the mountain ginseng into a powder, pass through a 75-85 mesh sieve, accurately weigh, add 65-75% methanol aqueous solution, the ratio of the weight g of the mountain ginseng to the volume mL of the methanol solution for 2:15-25. After weighing, ultrasonically extract at 45-55°C for 90-110 minutes, weigh again, and use 65-75% methanol aqueous solution to make up for the weight loss. Use qualitative filter paper for the first filtration, take 8-12mL of the subsequent filtrate, rotary evaporate to dryness, add 65-75% methanol aqueous solution to dissolve to constant volume, the ratio of the volume of the secondary filtrate before rotary evaporation to the volume of methanol added after rotary evaporation For 8-12:2. Then use a 0.22 μm filter membrane for the second filtration to obtain the test solution.

[0069] Preparation of the reference product solution: add the reference product to methanol a...

Embodiment 2

[0083] 1. Sample pretreatment

[0084] Preparation of the test solution: Take 2.0 g of the ground ginseng powder passed through an 80-mesh sieve, weigh it accurately, put it in a 25 mL conical flask with a stopper, add 20.0 mL of 70% methanol aqueous solution, weigh it, and place it at 50 ° C. Sonicate for 100 min, weigh again, and use 70% methanol aqueous solution to make up for the weight loss. Use qualitative filter paper for the first filtration, take 10.0mL of the subsequent filtrate, rotary evaporate to dryness, reconstitute with 70% methanol aqueous solution, transfer to a 2mL volumetric flask, dissolve with 70% methanol aqueous solution to volume, and then use a 0.22μm filter membrane Filter for the second time to obtain the test solution.

[0085] Preparation of the reference substance solution: put the reference substance with a content of 5.0mg in a 5mL volumetric flask, add methanol and dissolve to a constant volume to prepare the reference substance solution. Re...

Embodiment 3

[0099] Adopt the detection method of the fingerprint of mountain ginseng under the forest established in the above-mentioned embodiment 2 to detect 26 batches of samples of mountain ginseng under the forest, obtain the fingerprint of mountain ginseng under the forest, obtain the fingerprint of mountain ginseng under the forest with the same fingerprint detection condition The standard fingerprints of wild ginseng under the forest were evaluated for similarity, and the overlay of fingerprints was as follows figure 1 shown. The similarities between the fingerprints of 26 batches of ginseng samples and the standard fingerprints of ginseng under the forest were greater than 0.90.

[0100] At the same time, if figure 2 As shown, the standard fingerprint of mountain ginseng under the forest includes 33 common fingerprint peaks, with peak No. 2 as the positioning peak (S peak, relative retention time is 1.000), and the relative retention time of the other 32 common fingerprint peak...

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Abstract

The invention provides a detection method for a fingerprint spectrum of mountain ginseng in a forest. Test solution containing the mountain ginseng in the forest and reference solution containing a reference are determined by adopting high performance liquid chromatography, the obtained fingerprint spectra of the test solution and the reference solution are compared with a standard fingerprint spectrum of the mountain ginseng in the forest, and a common characteristic peak is pointed out according to relative retention time, so that the fingerprint spectrum of the mountain ginseng in the forest in the test solution is obtained. The invention further provides application of the detection method in quality detection and component content determination of the mountain ginseng in the forest. The detection method provided by the invention for the fingerprint spectrum of the mountain ginseng in the forest establishes the fingerprint spectrum of the mountain ginseng in the forest, the mountain ginseng in the forest can be distinguished from garden ginseng, quantitative analysis is performed on multiple components in the mountain ginseng in the forest, the current situation of the mountainginseng in the forest is comprehensively reflected, and quality control level of the mountain ginseng in the forest is improved.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of traditional Chinese medicine components, and relates to a method for detecting fingerprints of ginseng under forests. Background technique [0002] Ginseng under the forest is the dried root and rhizome of Panax ginseng C.A.Mey., a plant of the Araliaceae family. , invigorating the spleen and benefiting the lungs, calming the nerves and improving intelligence. Modern clinical pharmacological studies have shown that the main medicinal components of ginseng are ginsenosides, which have multiple medicinal effects. For example, Chen Jie et al. believe that ginseng can enhance immunity (research progress on the immune regulation of ginsenosides[J]. Shanxi Traditional Chinese Medicine Academic Journal, 2014(05):75-77), Jiang Weiwei and others believed that ginseng can anti-tumor (Ginsenoside Rb1, Rg3, Rh2 anti-tumor effect and mechanism overview[J]. World Science and Technology-Modern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 张艳欣王丹丹朱国琴吴咏梅陈丹青高崎
Owner SPH XING LING SCI & TECH PHARM CO LTD
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