The invention belongs to the technical field of flotation separation of arsenopyrite and copper sulfide ores, and particularly relates to an arsenopyrite combined inhibitor and an application thereof.The combined inhibitor comprises an organic inhibitor and an inorganic inhibitor. The organic inhibitor and Cu<2+> form a complex, and the complex is adsorbed on the surface of arsenopyrite, so the hydrophilicity of the surface of the arsenopyrite is enhanced, the floatability of the arsenopyrite is reduced, and the inhibition effect on the arsenopyrite is enhanced. Compared with single inhibitors, the combined inhibitor can greatly reduce the adsorbing capacity of a collecting agent on the surface of the arsenopyrite ore. The combined inhibitor has a high inhibition selectivity on arsenopyrite, and basically has no inhibition effect on the copper sulfide ore. The combined inhibitor has a high adaptability, and can effectively inhibit arsenopyrite in copper sulfide concentrate containingchalcopyrite and schreyerite. The combined inhibitor also has the advantages of small dosage, safety, non-toxicity, environmental friendliness, good stability, good water solubility, easiness in preparation and addition, convenience in use, effective achieving of flotation separation of the arsenopyrite and copper sulfide ores, and wide application prospect.