The invention relates to a method for rapidly detecting beta
receptor agonist in pork. The method comprises the steps of: 1) weighing a
pork sample, carrying out homogenizing and
enzymatic hydrolysis,adding an extracting solution, carrying out vortex oscillation for 10-15 minutes and centrifuging for 5-10 minutes at the speed of 8000rpm to obtain supernatant for later use; 2) After pretreating anMCX (Mixed-mode Cation eXchange)
solid phase extraction column, loading the supernatant of the step 1) onto the MCX
solid phase extraction column, washing by using deionized water, draining the MCX
solid phase under negative pressure, eluting the MCX
solid phase extraction column by using eluent, concentrating,
drying and diluting by using a 0.1% of
formic acid solution to obtain a sample liquid;and 3) loading the sample liquid onto an ultra-high performance liquid chromatograph to detect the content of the beta
receptor agonist in the sample liquid. The method disclosed by the invention hasthe advantages that the
enzymatic hydrolysis time is significantly shortened by optimizing the
enzymatic hydrolysis conditions; the
pork sample can be quickly pretreated through extraction and
elution; a satisfactory separation effect can be quickly obtained when the sample loading and detection are carried out by using the ultra-high performance liquid chromatograph of the method; and the methodis simple and convenient to operate, fast and high in efficiency and is suitable for the detection of large-batch samples.