65 results about "Pentose phosphate pathway" patented technology

A stent provided with a composition having at least one type of inhibitor of ATP synthesis, optionally together with at least one inhibitor of the pentose phosphate pathway is disclosed. The medical stent is useful for treating stenosis and preventing restenosis in vascular ducts and for treating cancerous tumors present in ducts, resectioned cavities and scars and any disorder arising from the proliferation of cells in ducts or cavities.

The present invention relates to a novel Saccharomyces cerevisiae strain utilizing xylose for fermenting ethanol expressing xylose isomerase (XI), overexpressing xylulokinase (XK), overexpressing the pentose phosphate pathway (PPP), and non-expressing aldose reductase (AR) and being adapted to growth in mineral defined medium with xylose as sole carbon source.

Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase ["G6PDH"] and 6-phospho-gluconolactonase ["6PGL"] in transgenic strains of the oleaginous yeast, Yarrowia lipolytica, comprising a functional polyunsaturated fatty acid ["PUFA"] biosynthetic pathway, resulted in increased production of PUFAs and increased total lipid content in the Yarrowia cells. This is achieved by increased cellular availability of the reduced form of nicotinamide adenine dinucleotide phosphate ["NADPH"], an important reducing equivalent for reductive biosynthetic reactions, within the transgenic microorganism.

The present invention comprises an in vitro enzymatic process that effectively converts renewable polysaccharides into high yields of hydrogen at mild conditions, using only enzymes and water. The process comprises a number of enzymes: (1) phosphorylases, (2) phosphoglucomutases, (3) hydrogenases, and (4) enzymes involved in the pentose-phosphate pathway. Preferred embodiments of the process produce only hydrogen and carbon dioxide as net products, translating into an inexpensive method of generating hydrogen in very large quantities from low-cost feedstocks.

The invention relates to a method for increasing content of docosahexaenoic acid (DHA) in schizochytrium limacinum grease. The method is characterized by comprising the following steps: lowering metabolic flux of a pentose phosphate pathway in thallus by selecting a fermentable carbon source, and lowering the metabolic flux of the pentose phosphate pathway in thallus by further adding an exogenous regulation factor; inhibiting the activity of malic enzyme in a tricarboxylic acid transfer system to greatly lower the content of nicotinamide adenine dinucleotide phosphate (NADPH) in the schizochytrium limacinum thaluus, promote the large-scale synthesis of DHA and enable the content of DHA in the grease of the obtained thallus to be as high as 55.3%, wherein the fermentation carbon source is 1,2-propylene glycol or any one or a mixture of 1,2-propylene glycol and acetate, the exogenous regulation factor is preferably sesamol which is added after filtering and degerming when a fermentation culture medium is sterilized, and the addition amount of the sesamol is 0.5-3.0mM / L. The method disclosed by the invention is simple and easy to perform, obvious in effect, capable of greatly increasing the content of DHA in the thallus grease and easy for industrial application.

The invention relates to the methods of manufacturing five-carbon sugars and sugar alcohols as well as other compounds derived from pentose-phosphate pathway from readily available substrates such a hexoses using metabolically engineered microbial hosts.

Disclosed herein is a newly discovered problem and solution for engineering S. cerevisiae to ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. The problem is that when grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in S. cerevisiae. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase from Bacteroides fragilis which is less inhibited by xylitol than other xylose isomerases, exemplified by E. coli xylose isomerase. Expression of the Bacteroides fragilis xylose isomerase may be used alone, or in combination with expression of a xylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.

The invention relates to a method for promoting synthesis of DHA in schizochytrium limacinum grease, and particularly relates to a method for prompting synthesis of docosahexaenoic acid (DHA) in schizochytrium limacinum grease. The method is characterized in that the activity of malic enzyme in a tricarboxylic acid transport system is inhibited by adding an exogenous control factor, so that the content of the NADPH in the schizochytrium limacinum can be greatly reduced, the synthesis of a great amount of DHA can be promoted, and the exogenous control factor is sesamol. According to the method, a fermentable carbon source also can be selected to reduce metabolic flux of a pentose phosphate pathway in the bacteria, so that the content of NADPH in the schizochytrium limacinum can be greatly reduced, the synthesis of a great amount of DHA can be promoted, and the largest content of the DHA in the grease of final bacteria can reach 55.3 percent. The method is simple, easy, obvious in effect, capable of greatly increasing the content of DHA in the bacteria grease and is likely to realize the industrialized application.

The invention relates to the field of producing succinate by E. coli fermentation. Specifically, the invention provides an engineered recombinant E. coli for producing succinate, wherein said E. coli contains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinant E. coli for producing succinate, and a method of using the engineered recombinant E. coli for producing succinate.