65 results about "Pentose phosphate pathway" patented technology

Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase ["G6PDH"] and 6-phospho-gluconolactonase ["6PGL"] in transgenic strains of the oleaginous yeast, Yarrowia lipolytica, comprising a functional polyunsaturated fatty acid ["PUFA"] biosynthetic pathway, resulted in increased production of PUFAs and increased total lipid content in the Yarrowia cells. This is achieved by increased cellular availability of the reduced form of nicotinamide adenine dinucleotide phosphate ["NADPH"], an important reducing equivalent for reductive biosynthetic reactions, within the transgenic microorganism.

Disclosed herein is a newly discovered problem and solution for engineering S. cerevisiae to ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. The problem is that when grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in S. cerevisiae. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase from Bacteroides fragilis which is less inhibited by xylitol than other xylose isomerases, exemplified by E. coli xylose isomerase. Expression of the Bacteroides fragilis xylose isomerase may be used alone, or in combination with expression of a xylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.

The invention relates to the field of producing succinate by E. coli fermentation. Specifically, the invention provides an engineered recombinant E. coli for producing succinate, wherein said E. coli contains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinant E. coli for producing succinate, and a method of using the engineered recombinant E. coli for producing succinate.