81 results about "Pathogen inactivation" patented technology

Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.

Methods and devices are provided for reducing the concentration of low molecular weight compounds in a biological composition, while substantially maintaining a desired biological activity of the biological composition. The device comprises highly porous adsorbent particles, and the adsorbent particles are immobilized by an inert matrix. The matrix containing the particles is contained in a housing, and the particles range in diameter from about 1 μm to about 200 μm. The matrix can be fibrous, and the particles can have a surface area greater than 750 m2 / g and a pore diameter between about 25 and 800 Å. The device can be used to adsorb and remove a pathogen-inactivating compound that is a nucleic acid-binding compound such as psoralen, an acridine derivative or a dye from a biological composition such as a blood product.

Methods are provided for improved quenching of undesired side reactions upon treating a red blood cell composition with a pathogen inactivating compound comprising a nucleic acid binding ligand and a functional group which is, or which is capable of forming, an electrophilic group. In some embodiments, the improved methods use a suitably high concentration of quencher that comprises a nucleophilic functional group that is capable of covalently reacting with the electrophilic group, wherein the treatment occurs within a desired pH range to provide sufficient quenching. Preferred quenchers for use in some of the methods include thiols, such as glutathione, which have been suitably neutralized such that addition to a red blood cell composition results in the desired concentration of quencher at a desirable pH range of 6.8 to 8.5.

A UV air cleaning and disinfecting system has an enclosed chassis with an inlet / outlet surface panel on one planar side thereof, and the chassis is mounted in the space behind a drop ceiling with its surface panel coplanar with the ceiling surface and facing into the room space. The surface panel has a pair of inlet vent arrays on opposite longitudinal sides of the surface panel from each other, and a pair of outlet vent arrays on opposite latitudinal sides of the surface panel from each other. Arranged in the chassis are an air blower unit and an array of UV lamps. The positioning of the inlet and outlet vent arrays establish four mutually circulating air streams in a four-leaf-clover pattern in the room space, which obtains a high-efficiency throughput of air handled by the system. The UV lamp array is arranged longitudinally across the chassis between the inlet ventsat a lower elevational position of the chassis. A pair of air filters is arranged at intake chambers separated by UV-blocking baffles at opposite longitudinal sides of the main chamber housing the air blower in the center of the chassis at an upper elevational position from the array of UV lamps. The upward and downward movement of the air through the filters and over the UV lamps toward the center causes swirling movements which enhance the exposure of pathogens in the air to UV radiation exposure. The surface panel is formed with a series of cascading door sections to allow wide access across the center of the chassis for maintenance of the UV lamps, with a first door section connected to an interlock switch for cutting off electrical power to the UV lamps and fan when the first door section is opened. The UV-blocking baffles have an angled shape and slits at an upper flange thereof covered with fused silica shields. The system's chassis is dimensioned to fit on ceiling rails spaced at standard 4 foot length and 2 foot width intervals. The system can provide 99% or higher inactivation of pathogens in the air with 2 to 7 or more air changes per hour for standard sized rooms.

Methods and devices are provided for reducing the concentration of low molecular weight compounds in a biological composition containing cells while substantially maintaining a desired biological activity of the biological composition. The device comprises highly porous adsorbent particles, and the adsorbent particles are immobilized by an inert matrix. The matrix containing the particles is contained in a housing, and the particles range in diameter from about 100 μm to about 1500 μm. The device can be used to adsorb and remove a pathogen-inactivating compounds from a biological composition such as a blood product.

The present invention provides compositions and methods of use thereof to prevent and / or treat pathogenic infection. In particular, the present invention provides the use of kinase inhibitors to inhibit kinases that involve in pathogen-host cell interactions that are associated with or cause pathogenic infections, therefore, to effectively prevent and / or treat pathogenic infections with far less likely to engender resistance as compared to conventional antibiotics and anti-viral drugs. The present invention further provides the use of kinase inhibitors for the treatment of acute pathogenic infections for a short period of time to avoid toxicities that may caused by long term use of these kinase inhibitors.

A method of oxidation or pathogen inactivation in water supplies using the combination of treatments of chlorine dioxide and ozone in the sequential steps prior to filtration and distribution. Results indicate that the addition of chlorine dioxide before prezonation reduces the bromate formation and chlorine dioxide can be substituted for preoxidation with ozone, while maintaining the enhanced filtration effects observed from the presence of a preoxidant. A majority of the chlorite formed from reactions of chlorine dioxide with constituents in raw water are converted to chlorate by the following ozonation step.

The present invention provides improved methods for treating red blood cell compositions with a pathogen-inactivating compound under conditions which provide suitable pathogen inactivation while maintaining cell vitality. Also provided methods of reducing dehydration in red blood cells, as well as treated red blood cell compositions.

The present invention is related to pre-connected disposable sets including a pre-connected bag containing a photosensitizer for use in a blood separation and pathogen inactivation procedure.

The presently-disclosed subject matter relates to antibodies, compositions, and methods for inhibiting and treating pathogen infection and providing contraception. In particular, the presently-disclosed subject matter relates to inhibiting and treating pathogen infection and providing contraception in a subject using compositions and antibodies capable of trapping pathogens or sperm in mucus, thereby inhibiting transport of pathogens or sperm across mucus secretions. The subject matter further relates to methods for monitoring the effectiveness of vaccines by detecting antibodies capable of trapping pathogens in mucus.

A method for reducing hemolysis and microparticle formation during storage of pathogen reduced blood. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced hemolysis. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles. Oxygen and pathogen reduced blood compositions comprising CPAD and riboflavin having reduced hemolysis. Oxygen and pathogen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles.