45 results about "Nitrate reductase (NADH)" patented technology

The invention discloses an underflow constructed wetland ecological system for disposing rural domestic waste and a disposing method. By adding root advantageous microorganisms in a canna water planting system, ratio of Alkaligenes, flavobacterium, lysobacter, Pseudomonas and other advantageous germs can be rapidly increased, the abundance of dioxygenase, dehydrogenase, phosphate dehydrogenase, urease, nitrate reductase, and dehydrogenase gene in catalase gene in the constructed wetland can be improved; two kinds of wetland plants can be planted in an intersecting manner, thus the different plant root secreta can be generated and a rich root microbial community structure is formed; the underflow constructed wetland ecological system can remove COD, ammonia nitrogen and total phosphorus atthe same time. Then the method of alternatively running continuous water feeding-intermittent water feeding is applied, thus the nitrogen removal efficiency is promoted while the system is stably operated. The method fully applies the advantages of the constructed wetland, strengthens the rapid construction of the constructed wetland ecological system, and optimizes the wetland running mode; the underflow constructed wetland ecological system is significant in environment, economic and social values.

The NR enzymes described herein were discovered in the red algae of Porphyra perforata (PpNR) and Porphyra yezoensis (PyNR). The present invention provides methods and compositions relating to altering NR activity, nitrogen utilization and / or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate reductase (NR) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NR enzyme show improved properties, for example, increased yield and growth.

The invention relates to an analysis method of testing the nitrate reductase activity in the soil, which includes the following steps: 1) n dried and screened soil samples are weighed, adopted and put into test tubes; 2, 4-dinitrophenol solution is added into the test tubes and mixed to be even; and then substrate potassium nitrate solution is added into n-1 test tubes and the same amount of distilled water is added into the other one test tube for sample comparison; glucose solution is respectively added into the test tubes, which is then supplemented by distilled water to be 5 to 10ml and cultivated under constant temperature; 2) the distilled water is added to supplement to 50 to 100ml; 3) potash alum saturated solution is added in to be oscillated and filtered; 4)the distilled water and a chromogenic reagent are added into the filtrate, and a light absorption value A is determined under 520nm; 5) the content of nitrite in the samples is calculated. The analysis method has the advantages of: 1) simplification of operation steps and reduction of the complicacy and uncertainty during the anaerobic culturing process; 2) reduction to dependency on the equipment requirements; 3) high accuracy and easiness in operation; 4) stable and reliably result, and good repeatability.

The invention relates to a method for inhibiting generation of nitrite ion or nitrite in health care products, food or medicinal material containing nitrate ion or nitrate and nitrate reductase. The method comprises inhibiting the activity of nitrate reductase in the health care products, food or medicinal material by using the health care food, food or medically accepted nitrate reductase. The invention particularly relates to a method for inhibiting generation of nitrite ion or nitrite in cubilose, comprising using cubilose-accepted nitrate reductase inhibitor to inhibit the activity of the nitrate reductase in cubilose. Particularly, the method comprises adding the cubilose-accepted nitrate reductase inhibitor with effective inhibiting amount in newly collected cubilose or adding cubilose-accepted nitrate reductase inhibitor with the effective inhibiting amount in the cubilose production process. The nitrate reductase inhibitor can be selected from tungstate. By adopting the method, generation of nitrite ion or nitrite in cubilose can be effectively inhibited, and the edible risk of the cubilose can be lowered.

The invention discloses isoptericola and a method for using the isoptericola for synthesizing nano silver. The strain is Isoptericola sp. SYSUZL-3, preserved in China Center for Type Culture Collection on January 5, 2017 under the accession number of CCTCC NO:2017006. The strain has the ability to produce nitrate reductase to reduce silver ions for further synthesizing the nano silver. The strainSYSUZL-3 is activated, cultured, and centrifuged to obtain supernatant. A silver nitrate solution is added to the supernatant, and the nano silver is obtained by light irradiation. The nano silver hassmall particle size and high yield, and has obvious inhibitory effect on the growth of pathogenic bacteria such as Staphylococcus warneri, and sunlight is used to catalyze the reaction, and the synthesis efficiency is greatly improved. The synthesis method is low in production cost, simple in operation, non-pollution to the environment, and green, and has a good application prospect.

The invention discloses a transcription factor derived from corn and new use of a coding gene thereof. The new use provided by the invention is a method using a coding nucleic acid molecule of ZmNLP1;1 to cultivate a transgenic plant. The method is a method for cultivating the transgenic plant with at least one characters of the following 1)-4): 1) aboveground biomass is higher than that of a receptor plant; 2) after induction of a nitrate, the nitrate transport protein gene expression is higher than that of the receptor plant; 3) after induction of the nitrate, nitrate reductase gene expression is higher than that of the receptor plant; and 4) after induction of the nitrate, nitrite reductase gene expression is higher than that of the receptor plant; and the method comprises a step for introducing a ZmNLP1;1 gene into the receptor plant; and the ZmNLP1;1 gene codes a protein shown in SEQ ID No.2. Experiments show that, in the condition of using nitric nitrogen (NO<3->) as a sole nitrogen source, the aboveground biomass and the total biomass of arabidopsis transgenic with the ZmNLP1;1 gene are increased significantly compared with that of receptor arabidopsis.

The invention relates to clone and application of a loose leaved Chinese cabbage nitratase gene BcNR, belonging to the molecular biology and biotechnology field. Total RNA is extracted from a loose leaved Chinese cabbage leaf and then 2 gamma of the total RNA is reversely transcribed into cDNA; three pairs of special primers are designed according to conservative nucleotide sequences in an NIAI (mouseearcress) and an NR (cabbage type rape) for routine polymerase chain reaction (PCR); PCR products are respectively connected to pMD18-T vectors; DH5alpha cells are transformed and sequencing is performed; full-length cDNA is obtained through quick amplification of 3' terminals and 5' terminals; a sense expression vector is further constructed and the NIAI is transformed. The transgenic NIAI has high nitratase transcription level. The genetic transformation vegetable can adjust the content of nitrate in the vegetable and improve the output and the quality and have very important economic benefit and social benefit.

The invention provides a growth regulator for the fruiting period of peanuts. The growth regulator comprises the following components in percentage: 30-35% of growth promoter and 65-70% of growth nutrient solution. The regulator can favorably remove oxygen free radicals and delay senility; and the regulator obviously improves the activity of leaf nitrate reductase and the activities of glutamine synthetase and glutamate synthetase, and improves the activities of leaf sucrose synthetase and sucrose phosphate synthetase, thereby being beneficial to the accumulation of dry matters in pods and the synthesis of protein and fat in seed kernels. The growth regulator obviously increases the yield of peanut pods; and the yield is increased because the weight of a single pod is obviously increased, the full-pod rate is improved, and the dual-kernel pod rate and the kernel rate are greatly improved.

The present invention is related to the field of reddening of food products. In particular the present invention relates to a two-phase fermentation method for boosting the nitrate reductase activity of Staphylococcus strains with nitrate reductase activity comprising a first aerobic phase in the absence of nitrate and a second anaerobic or oxygen limited phase with continuous nitrate feeding and use of the Staphylococcus strains for reddening of meat products.

The invention provides a degrading strain capable of eliminating norfloxacin left in wastewater and belongs to the technical field of high techniques for biology. The strain is gram staining reaction negative bacteria NOR-36 and is identified as acinetobacter calcoaceticus. The acinetobacter calcoaceticus is preserved in CGMCC (China general microbiological culture collection center) in Microbiology Research Institute of China Science Academy in No.3, yard No.1, Beichen western road, Chaoyang district, Beijing, the postcode is 100101, the collection number is CGMCC No.10540, and the strain is identified as acinetobacter calcoaceticus. The main biological character is gram-negative, the bacterial colony is smooth, and the strain is short-rod-shaped and is positive when contacted with oxidase and negative when contacted with nitrate reductase. The degrading product can be directly applied and can reduce the amount of residual norfloxacin in water by more than 90%.

The invention relates to a bacillus megaterium nitrate reductase catalysis-electron transfer subunit coexpression carrier in the technical field of genetic engineering. The coexpression carrier is constructed by the following steps: firstly determining a bacillus megaterium whole genome sequence as a template, amplifying into a gene sequence containing enzyme cutting site nitrate reductase catalysis subunit (Bm-Nas B) and electron transfer subunit (Bm-Nas C) according to a designed PCR (Polymerase Chain Reaction) primer, and sequentially connecting the gene sequence onto the coexpression carrier pETDuet-1, and selecting positive clone of escherichia coli DH5alpha containing target gene fragment through a colony PCR technology. According to the bacillus megaterium nitrate reductase catalysis-electron transfer subunit coexpression carrier, the problem that the expression quantity of the nitrate reductase catalysis-electron transfer subunit gene sequence in the prior art is low in the natural bacillus megaterium and the usage effect is seriously restricted can be overcome, and the great increase on the expression quantity of the gene can be realized.

The present invention is related to the field of reddening of food products. In particular the present invention relates to the preservation of nitrate reductase activity of frozen and / or dried lacticacid bacteria culture or Micrococcaceae culture.

The invention provides staphylococcus equorum FS310 derived from Xuanwei ham, which is in CGMCC (China General Microbiological Culture Collection Center) No.24492. The strain is high in production adaptability (high salt resistance, nitrate resistance and acid resistance), has the biological characteristics of high growth speed, high acid production capacity and the like, and can be used for preparing high-yield acid-resistant bacteria. Due to the protease activity, nitrate reductase activity, relatively strong antioxidant enzyme activity and biological safety, the bacillus amyloliquefaciens has huge application potential; when the staphylococcus equorum FS310 is applied to fermentation of hams, sausages and aquatic products, color development of meat products can be obviously promoted, the residual amount of nitrite is reduced, fat peroxidation is inhibited, the texture and flavor of the meat products are improved, and the staphylococcus equorum FS310 has a good application prospect in production of fermented meat products as a leavening agent for meat.

The invention provides a degrading bacterial agent for eliminating norfloxacin residues in waste water, which belongs to the technical field of biological high technology. The strain used was Gram-negative bacteria NOR‑36, which was identified as Acinetobacter . calcoaceticus. It was deposited in the General Microbiology Center of China Microbial Cultures Preservation Management Committee (CGMCC for short, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101), on February 9, 2015, with a deposit number CGMCC NO.10540, identified as Acinetobacter calcoaceticus. The main biological characteristics are negative Gram stain, smooth, short rod-shaped colonies, positive for contact oxidase, and negative for nitrate reductase. The direct application of degrading bacteria products can reduce the residual amount of norfloxacin in water by more than 90%.

The invention belongs to the technical field of soil nitrogen cycle determination, and discloses a novel method for measuring activity of soil nitrate reductase. The method comprises the following steps: taking potassium nitrate (KNO3) as a substrate, carrying out reduction culture on soil for 24 h at a constant temperature of 30 DEG C under an anaerobic condition, measuring the contents of nitrate nitrogen (NO3<->-N) in the soil before and after reduction, calculating the difference value to obtain the amount of reduced NO3<->-N in each gram of dry soil, and characterizing the nitrate reductase activity of the soil by the amount of the reduced NO3<->-N. In a culture experiment, a common conical flask and a vacuum container are used for replacing a decompression conical flask, and a continuous flow analyzer is used for replacing a phenol disulfonic acid colorimetric method to measure the NO3<->-N content. Compared with the prior art, the method can remarkably reduce experimental errors, and improve the accuracy and test efficiency of measurement results.

The invention discloses a rice nitrate reductase gene NR2. The sequence of the gene may be: (a) a genomic nucleotide sequence represented by Seq ID No.1; (b) a cDNA nucleotide sequence represented by Seq ID No.2; or (c) nucleotide sequence of a mutant gene, allele, derivative thereof or the like, which is formed by adding, substituting or losing one or several nucleotides in the nucleotide sequence (a) or the nucleotide sequence (b) and can code a protein having nitrate reductase activity. The invention also discloses a protein (with an amino acid sequence represented by Seq ID No.3) coded bythe gene and the use of the gene in regulation of absorption and utilization of nitrogen. Through the cloning and use of the NR2 gene, the absorption and use of nitrogen by rice can be effectively regulated, the efficiency of nitrogen utilization can be improved, and finally the yield of the rice can be increased.

A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).

The invention discloses a rice nitrate reductase NIA3 protein gene OsNia3. The rice nitrate reductase NIA3 protein gene OsNia3 has a cDNA sequence shown as SEQ ID NO. 1 which encodes a rice NIA3 protein having an amino acid sequence shown as SEQ ID NO. 2. Homozygous mutants with rice gene OsNia3 knocked-out and homozygous stains with OsNia3 overexpression are obtained by utilizing a transgenic technology. It has been discovered by analysis that the stains with the OsNia3 gene knocked-out have shortened plant height, shorted growth period and reduced yield per plant; however, the stains with OsNia3 overexpression have relatively high plant height, prolonged growth period and increased yield per plant. Thus, the rice nitrate reductase NIA3 protein gene OsNia3 can be introduced into plants asa target gene, thereby improving nitrogen use efficiency of the plants so as to be subjected to plant variety improvement.

The invention discloses a molecular diagnostic method for nitrogen nutrition of field potato plants. Specifically speaking, a nitrate reductase (NR) gene related to nitrogen absorption by potato plants is used as a molecular marker for judging the states of nitrogen nutrition of field potato plants. During diagnosis, RNA of a field potato plant is used as a template, reverse transcription is carried out to obtain cDNA, the expression level of the NR gene is detected by using real-time PCR, the state of nitrogen nutrition of the field potato plant is judged according to the expression level of the NR gene, and whether to carry out topdressing of a nitrogenous fertilizer and the amount of topdressing of the nitrogenous fertilizer can be further determined. The diagnostic method has the advantages of good accuracy in judgment, high sensitivity, etc.

The invention relates to the technical field of plant genetic engineering and provides a transcription factor ZmNLP4 derived from corn and application thereof. A nucleotide sequence of the transcription factor is shown as SEQ ID NO.1, and a coded amino acid sequence of the transcription factor is shown as SEQ ID NO.2. Compared with a receptor Arabidopsis, the Arabidopsis in which the transcriptionfactor is transferred has the advantages that the total biomass is obviously increased, the content of in-vivo amino acids and total nitrogen is increased, the main root length and quantity of lateral roots are obviously increased, the yield per plant is obviously increased, and the expressions of nitrate transporters, nitrate reductase genes and nitrite reductase genes are obviously increased.

The NR enzymes described herein were discovered in the red algae of Porphyra perforata (PpNR) and Porphyra yezoensis (PyNR). The present invention provides methods and compositions relating to altering NR activity, nitrogen utilization and / or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate reductase (NR) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NR enzyme show improved properties, for example, increased yield and growth.

A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).

The invention relates to engineering bacteria based on nitrate reductase and an implementation method of the engineering bacteria. The method includes the steps of firstly, conducting PCR amplification on primers containing enzyme cutting sites with the streptomyces griseorubens genome DNA as a template to sequentially obtain the nucleotide sequence of encoding nitrate reductase catalysis subunits and the nucleotide sequence of electron transfer subunits; secondly, sequentially connecting the gene sequence obtained through amplification to the co-expression vector pETDuet-1; thirdly, obtaining the recombination co-expression vector pETDuet-NB-NA; fourthly, converting the nitrate reductase expression vector into escherichia coli expression strains. In order to overcome the defects that nitrate reductase is generally endoenzyme in vivo and the expression quantity is low, the application range and the effect are seriously limited, a large amount of nitrate reductase can be expressed and synthesized in vitro through the genetic engineering means. The engineering bacteria and the method have great significance in rapid repair of secondary salinization soil and even the achievement of precision agriculture.

The invention discloses a nitrogen nutrition molecular diagnosis method for field potato plants. Specifically, the nitrogen absorption related gene nitrate reductase gene (NR) of potato plants is used as a molecular marker for measuring the nitrogen nutrition status of field potato plants. During the diagnosis, the RNA of the field potato plant was used as the template, the cDNA was obtained by reverse transcription, and the Real? time? The expression level of NR gene was detected by PCR. According to the expression level of NR gene, the nitrogen nutrition status of field potato plants could be judged, and then whether topdressing nitrogen fertilizer and its application rate could be determined. The diagnostic method has the advantages of good judgment accuracy and high sensitivity.

The invention belongs to the field of microorganisms, and discloses a strain of Pseudomonas stutzeri ZH‑14 (Pseudomonas stutzeri ZH‑14) with aerobic denitrification function, and its preservation number is CCTCC NO: M2018730. The above Pseudomonas stutzeri can be applied to degrade ammonia nitrogen or nitrate nitrogen or nitrite nitrogen or COD in nitrogenous sewage. Beneficial effects: Pseudomonas stutzeri ZH‑14 has both aerobic denitrification and heterotrophic nitrification functions, and the degradation process contains extremely low levels of nitrite nitrogen accumulation; it can directly use nitrite for growth and metabolism ;Pseudomonas stutzeri ZH‑14 has a relatively strong periplasmic nitrate reductase activity in a low carbon-to-nitrogen ratio environment, and the aerobic expression is less affected by the type of carbon source used, which solves the problem of using aerobic Carbon source problem in the process of denitrifying bacteria treating nitrogenous sewage.

The invention relates to a method for measuring ammonia (ammonia ion) content by using a double enhance method, an enzymatic colorimetric method and a couple reaction technology, and components and constituents of a reagent. The technical principle of the measurement is finished according to series catalytic reaction of ferredoxin-nitrite reductase, nitrate reductase and nitric oxide dioxygenase. The invention also relates to an ammonia (ammonia ion) diagnosis / measurement kit. The measurement method of the invention is high in sensitivity and low in error; therefore, the measurement method and the kit can be widely applied to clinical medical examination / food inspection.

The invention belongs to the field of microorganisms and biotechnology, and in particular relates to an improved compound microorganism inoculum and its application in increasing the yield of Angelica sinensis. The composite microbial inoculum is compounded by Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas psychrophilic and Zygomycota; Functional characteristics, and improve the transformation, absorption and utilization of soil nutrients; and the compound microbial inoculum can not only promote the conversion of ammonium nitrogen in the rhizosphere soil to nitrate nitrogen, reduce the toxic effect of soil ammonium salt on plants, and improve It can also enhance the activity of nitrate reductase in plants, promote the conversion of nitrate nitrogen in plants to ammonium nitrogen, promote the absorption and enrichment of nitrogen by plants, and improve plant yield.

The invention discloses a construction method and application of an oxygen response type biosensor, and belongs to the field of synthetic biology. Based on fumarate nitrate reductase FNR and a promoter PfnrF8 containing an FNR targeted binding site, a biosensor capable of activating anaerobic metabolic pathway related genes is established, and meanwhile, the intensity of the promoter is adjusted through promoter engineering, so that biosensors with different response intensities are obtained. Therefore, a remarkable positive effect is achieved when the strain is applied to optimized fermentation for regulating and controlling escherichia coli to biologically synthesize adipic acid. The dynamic regulation and control on the gene expression level along with the change of the fermentation environment are realized, the method is different from the traditional static fermentation with human intervention, and the method provides a beneficial reference for the production of other high-added-value compounds.