Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Modulation of nitrate levels in plants via mutation of nitrate reductase

A nitrate, reductase technology, applied in the directions of oxidoreductase, plant products, plant genetic improvement, etc., can solve problems such as low nitrite levels, and achieve the effect of overcoming the difficulties of growth and commercialization

Pending Publication Date: 2021-08-17
PHILIP MORRIS PROD SA
View PDF20 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, endogenous nitrite levels in plant tissues are usually very low due to its cytotoxicity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modulation of nitrate levels in plants via mutation of nitrate reductase
  • Modulation of nitrate levels in plants via mutation of nitrate reductase
  • Modulation of nitrate levels in plants via mutation of nitrate reductase

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0072] In one aspect, an isolated polynucleotide sequence encoding a nitrate reductase polypeptide comprising the contiguous polypeptide sequence of SEQ ID NO: 5 is provided, wherein methionine is reduced compared to a control plant cell Amino acid substitutions for nitrate reductase activity.

[0073] Suitably, the nitrate reductase polypeptide has the contiguous polypeptide sequence of SEQ ID NO: 7, wherein methionine is substituted with an amino acid that reduces nitrate reductase activity compared to control plant cells.

[0074] Suitably, the isolated polynucleotide sequence encodes a nitrate reductase polypeptide in which methionine is substituted by a different amino acid, preferably an aliphatic non-polar amino acid, more preferably isoleucine .

[0075] Suitably, the aliphatic non-polar amino acid is selected from the group of amino acids consisting of glycine, alanine, proline, isoleucine, leucine or valine.

[0076] Suitably, the aliphatic non-polar amino acid is ...

Embodiment

[0184] M0 seeds of Nicotiana vulgaris AA37 were treated with ethyl methanesulfonate (EMS) at different concentrations and exposure times to generate plant populations with random point mutations. Kill curves were estimated for each treatment at M1 generation, along with lethality, fertility and mosaicism. Selfing of the M1 plants produces seeds of the M2 family to allow the recessive allele to be recovered as homozygotes and the lethal allele to be recovered as heterozygotes. Genomic DNA was extracted from eight M2 plants per family of the EMS mutagenized population and screened for mutants, while M2 plant material and M3 seeds were collected and stored for future analysis. To identify and characterize mutant variants, genomic DNA samples from M2 plants were pooled in groups and screened by sequencing of targeted gene fragments. The tobacco NIA2 gene-specific primers shown in Table 2 were used to amplify the target gene fragment. Mutations in target genes are retrieved by se...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).

Description

technical field [0001] The present invention relates to plants having reduced nitrate levels, plant cells derived from said plants, products produced from said plants, and methods of modulating nitrate levels in plants by mutating nitrate reductase enzymes. Background technique [0002] Certain plants, such as tobacco plants, accumulate high levels of free nitrate in their leaves, which is undesirable because high levels of nitrate have been associated with carcinogenic compounds known as tobacco-specific nitrosamines (TSNAs) formation is associated. TSNAs are a class of compounds primarily produced during tobacco leaf curing, but additionally formed by high-temperature synthesis during subsequent leaf processing and storage and possibly during combustion. Two TSNAs found in dried leaves are N-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), classified as a Group I carcinogen (the highest level) by the International Agency for Research on ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/12A01H6/82C12N9/06A24B15/00
CPCA01H5/12A01H6/823A24B15/00C12N9/0044A01H1/06A01H1/10A24B3/12A24B15/10C12N15/8243C12Y107/01001
Inventor L·博维特P·坎帕尼S·格普费特
Owner PHILIP MORRIS PROD SA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com