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Isoptericola and method for using isoptericola for synthesizing nano silver

A technology of termite bacteria and nano silver, which is applied in the field of termite bacteria and its synthesis of nano silver, can solve the problems of inability to meet the actual demand of nano silver, low yield and yield of nano silver, and achieve good industrialization potential and application prospects, efficiency and efficiency. The effect of improved quality and good application prospects

Pending Publication Date: 2018-04-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield and yield of nano-silver prepared by biosynthesis are relatively low at present, which cannot meet the actual demand of nano-silver.

Method used

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  • Isoptericola and method for using isoptericola for synthesizing nano silver
  • Isoptericola and method for using isoptericola for synthesizing nano silver
  • Isoptericola and method for using isoptericola for synthesizing nano silver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Isolation and identification of bacterial strain SYSUZL-3

[0061] 1. Isolation and screening of strain SYSUZL-3

[0062] The strain SYSUZL-3 was isolated from the Xinjiang halophyte Idiophysis ( Borszczowia aralocaspica Bge.) root, after the surface of the plant is sterilized, weigh 1 g of the sample crushed by a blender, continue to grind it further with a sterile mortar, then add 9 mL of sterile water to the sample, and draw 1 mL of the tissue The suspension was serially diluted to 10 -2 、10 -3 、10 -4 Afterwards, the suspensions of each dilution concentration were taken to coat the separation plate.

[0063] Place the separation plate in an incubator at 30°C for 30-50 days. After the bacterial colony grows, pick and inoculate it on 1 / 2 YIM 38# purification medium, and use the three-section line method to purify; according to the culture characteristics of the strain on 1 / 2 YIM 38# medium, including the color of the colony , size, character, texture,...

Embodiment 2

[0079] Example 2 Optimization of process conditions for synthesizing nano silver by termite fungus strain SYSUZL-3

[0080] 1. Comparison of the efficiency of synthesizing nano-silver between the supernatant of the strain SYSUZL-3 and the bacteria

[0081] Use TSB liquid medium with a pH of 7.5 to cultivate the strain SYSUZL-3 at 28°C and 180 rpm. After culturing for 3 days, centrifuge at 10,000 rpm for 10 min. Take 5ml of the supernatant and bacterial cells and add them to 100ml of silver nitrate solution. , cultivated at 28°C and 180rpm for 24 h, and observed the discoloration of the solution.

[0082] Among them, the formula of TSB liquid medium is: tryptone 15 g, soybean peptone 5 g, NaCl 5 g, supplemented with distilled water to 1 L, pH 7.5, autoclaved at 121 °C for 25 min.

[0083] The experimental results showed that the production efficiency of nano silver synthesized by using the supernatant of strain SYSUZL-3 was higher.

[0084] 2. Effect of the volume ratio of st...

Embodiment 3

[0093] Example 3 Synthesis of silver nanoparticles using termite fungus strain SYSUZL-3

[0094] 1. Synthesis method

[0095] According to the optimization result of embodiment 2, utilize the optimal method of termite bacterial strain SYSUZL-3 synthetic nano-silver as follows:

[0096] (1) Obtain the supernatant of termite strain SYSUZL-3

[0097] After the termite strain SYSUZL-3 was activated twice, it was inoculated in a Erlenmeyer flask filled with 100 mL of TSB liquid culture, and fermented at 28°C and 180 rpm for 24 h. The fermentation broth was centrifuged at 10,000 rpm for 10 min and then filtered to remove bacteria. body, the strain SYSUZL-3 supernatant can be obtained.

[0098] Among them, the TSB liquid medium was: tryptone 15 g, soybean peptone 5 g, NaCl 5 g, supplemented with distilled water to 1 L, pH 7.5, autoclaved at 121 °C for 25 min.

[0099] (2) Synthetic nano silver

[0100] According to the volume ratio of 1:20, the supernatant of the strain SYSUZL-3 ...

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Abstract

The invention discloses isoptericola and a method for using the isoptericola for synthesizing nano silver. The strain is Isoptericola sp. SYSUZL-3, preserved in China Center for Type Culture Collection on January 5, 2017 under the accession number of CCTCC NO:2017006. The strain has the ability to produce nitrate reductase to reduce silver ions for further synthesizing the nano silver. The strainSYSUZL-3 is activated, cultured, and centrifuged to obtain supernatant. A silver nitrate solution is added to the supernatant, and the nano silver is obtained by light irradiation. The nano silver hassmall particle size and high yield, and has obvious inhibitory effect on the growth of pathogenic bacteria such as Staphylococcus warneri, and sunlight is used to catalyze the reaction, and the synthesis efficiency is greatly improved. The synthesis method is low in production cost, simple in operation, non-pollution to the environment, and green, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation and its application, and more specifically relates to a termite fungus and a method for synthesizing nano-silver. Background technique [0002] Nanoparticles refer to particles with a particle size between 1 and 100nm (nanoparticles are also called ultrafine particles), which belong to the category of colloidal particle size. They are in the transition zone between atomic clusters and macroscopic objects, between microscopic systems and macroscopic systems, and have unique optoelectronic and chemical properties. At present, a large number of studies have proved that nanoparticles have good antibacterial properties, and have been widely used in pharmaceutical carriers, water purification, antibacterial coatings, catalysis and other fields. As a traditional fungicide, silver has the advantages of high efficiency, safety, non-toxicity, broad antibacterial spectrum, and no drug resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/06C12P9/00A01N59/16A01P3/00A01P1/00C12R1/01
CPCA01N59/16C12N9/0044C12P9/00C12N1/205C12R2001/01
Inventor 李文均董周焱迈尼克
Owner SUN YAT SEN UNIV
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