The invention discloses a preparation method of a directional EPC (
endothelial progenitor cell) sample
cell. The preparation method comprises the following steps: S1, collecting fat, and separating fat cells; S2, culturing the fat cells by virtue an ADSCs (adipose
derived stem cells) culture medium to obtain a P2-generation ADSCs; and S3, culturing the P2-generation ADSCs by virtue of an EPCs (endothelial
progenitor cells) culture medium to obtain a directional EPC sample
cell. The directional EPC sample
cell, the preparation method and the application have the beneficial effects adipose-derived stromal cells (ADSCs) are pre-induced for three days by adopting an endothelial
progenitor cells culture medium to obtain heterogeneous
CEL (committed EPC-like) with CD133 expression of 47.63+ / -12.50 percent and vWF expression of 31.10+ / -5.32 percent, and the
CEL can increase the
blood supply of an injured part, can alleviate ischemic symptom, and is not only beneficial for the structural restoration of an injured area, but also beneficial for alleviating the functional injury of a
neurological function; and the
CEL restores the
blood supply by rebuilding a tissue structure of the injured area, thereby inhibiting the infection environment, reducing the
neuronal apoptosis, promoting axonal regeneration and synaptic connection, and restoring partial feeling and movement function.