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Use of miRNA-34a

A technology of 1.mirna-34a, 2.mirna-34a, applied in the field of biomedical materials

Inactive Publication Date: 2007-12-19
EAST CHINA NORMAL UNIV +1
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  • Abstract
  • Description
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But so far there is no literature report about the use of miRNA-34a in anti-neuron apoptosis

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Embodiment Construction

[0016] 1. RNA extraction: Add 1ml Trizol per 100mg of tissue, grind with liquid nitrogen to fully grind the tissue pieces. Add about 1 / 5 of the volume of chloroform, mix well by inverting up and down for about 1 minute, and let stand at room temperature for 5 minutes. After centrifugation at 12,000 rpm for 15 minutes at 4°C, carefully transfer the supernatant into a new 1.5ml centrifuge tube, add an equal volume of isopropanol, mix by inverting gently, and let stand at room temperature for 10 minutes. After centrifugation at 12000 rpm at 4°C for 10 minutes, the supernatant was removed, 2 / 5 volume of 70% ethanol was added to the precipitate, and the precipitate was washed by centrifugation at 12000 rpm at 4°C for 5 minutes. Remove the supernatant, dry the precipitate at room temperature, add an appropriate amount of RNase-free water to fully dissolve, and measure the OD260 and OD280 values. Using RNase-free DNase I treatment, the QIAGEN RNeasy(R) kit was used to purify the tot...

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Abstract

The present invention relates to use of small RNA molecule, is especially use of miRNA-34a, and belongs to the field of biomedicine material technology. By means of Northern process and other process, miRNA-34a is determined to be the age related expression, and by means of the contrast to the prefrontal lobe cortical tissue miRNA' s of rats in different age sections, it is found that the expression of miRNA-34a in the pallium of rhesus and the prefrontal lobe cortex of rat exhibits age dependent change. The present invention establishes a rat neuron AD cell model for the research of possible action mechanism of miRNA-34a. By means of further transfecting miRNA-34a precursor, miRNA-34a antisense RNA and other contrasts, it is found that miRNA-34a possesses neuron apoptosis effect of antagonizing A-beta induction.

Description

technical field [0001] The invention relates to the use of small RNA molecules, in particular to the use of miRNA-34a, and belongs to the technical field of biomedical materials. Background technique [0002] In recent years, a non-coding single-stranded RNA small molecule (microRNA, miRNA) widely exists in cells from plants, nematodes to humans, and they can be incompletely complementary to specific regions of the 3' untranslated end of their target mRNAs. interact and inhibit protein synthesis. In 1993, Lee et al. cloned the lin-4 gene for the first time in C. elegans using the classical cloning method, and confirmed the existence of small molecule RNA by the method of point mutation. In 2000, Reinhart et al. discovered the existence of the let-7 gene in humans and Drosophila, and speculated that this small molecule may be a class of evolutionarily conserved regulatory molecules that play an important role in the life process. Subsequently, more than a thousand miRNA gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P25/28A61P35/00
Inventor 赖立辉戚艳婷陈小娜张智李丹顾吉轶付笑男张亮
Owner EAST CHINA NORMAL UNIV
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