The invention discloses a traditional Chinese medicine formula with effects of resisting senility, removing wrinkles and lifting and tightening skin and a preparation method of the traditional Chinesemedicine formula, and belongs to the technical field of human health care products. The traditional Chinese medicine formula adopts the following raw materials: 5-15 parts of radix ginseng, 10-30 parts of lucid ganoderma, 1-5 parts of saffron, 2-10 parts of semen coicis, 5-20 parts of herba leonuri, 3-15 parts of radix astragali, 3-15 parts of rhizoma polygonati, 5-25 parts of radix angelicae sinensis, 10-30 parts of peach blossom, 4-15 parts of fructus lycii, 4-15 parts of flos chrysanthemi, 3-15 parts of rhizoma atractylodis macrocephalae, 3-15 parts of rhizoma bletillae, 3-15 parts of radix paeoniae alba, 5-15 parts of semen armeniacae amarum, 4-15 parts of poria cocos, 1-3 parts of fructus kochiae and 1-3 parts of cortex dictamni. Experiments prove that the traditional Chinese medicine formula has remarkable wrinkle removing effect; after 30-day use, the skin texture can be improved obviously and skin is enabled to be fine, smooth and white; after 90-120-day use, under-eye puffiness, wrinkles on the forehead, wrinkles and folds on forehead and glabella, crow's-feet, nasolabial folds and wrinkles on the neck can be removed substantially; the raw materials are obtained easily, the preparation method is convenient, and the traditional Chinese medicine formula has good application prospect and market prospect.