The invention belongs to the technical field of
plant tissue culture and discloses a
Castanopsis hystrix high efficiency isolated culture
plant regeneration method. Through explant treatment, basic medium design and optimization of different
hormone types and a concentration level ratio, a
Castanopsis hystrix select tree seed is used as an explant and is subjected to adventitious
bud induction,
bud propagation culture and rooting culture to form a complete
plant. The invention discloses a
Castanopsis hystrix tissue culture rooting
seedling transplanting matrix and a management technology of a transplanted
seedling. The method fills the blank of
tissue culture rapid propagation of a Castanopsis hystrix select tree seed as an explant, provides a stable and efficient Castanopsis hystrix isolated culture plant regeneration method, can be industrialized, has a low production cost and can culture strong, tidy and uniform Castanopsis hystrix seedlings. Castanopsis hystrix belongs to precious
tree species. At present, an
afforestation area is large and a market high-quality nursery stock is deficient. The Castanopsis hystrix high efficiency isolated culture plant regeneration method can provide
technical support and fine breeding guarantee for fast promotion of high-quality Castanopsis hystrix
germplasm and improvement of Castanopsis hystrix artificial forest
economic benefits and has a wide application prospect.