43 results about "Mandelonitrile" patented technology

The foliage and stems of plant species from the family Rosaceae, genus Prunus, yield natural pesticides when macerated. Hydrodistillation of macerated plant biomass yields a concentrated solution of organic volatile compounds that act synergistically as a natural pesticide. Volatile compounds liberated from Prunus biomass include 2-propanol, hexanal, trans-2-hexenal, 1-hexanol, cis-3-hexenol, mandelonitrile, benzoic acid, benzaldehyde, benzyl alcohol, hydrocyanic acid and others. These compounds may be removed from the distillate and reformulated to form a standard concentrated solution, with benzaldehyde, mandelonitrile and hydrogen cyanide being the major components. The extracts may be used as a soil treatment or soil fumigant for soil-borne pests. They also may be formulated for application as solutions with or without a surfactant or formulated as powders for foliar treatment. In a particular application, such extracts may be applied to postharvest commodities such as fruits, vegetables, roots, grains and nuts to protect against certain fungi and insects.

A multilayer wiring substrate which is high in connection reliability is provided through process steps of forming more than one opening, such as a via-hole in a dielectric layer laminated on a substrate, and then applying uniform copper plating to a surface portion of the dielectric layer including the opening to thereby form a wiring layer. An electroless copper plating solution with at least one of mandelonitrile and triethyltetramine mixed therein is used to perform the intended electroless copper plating. An alternative process makes use of an electroless copper plating solution with chosen additives or “admixtures” containing at least on of mandelonitrile and triethyltetramine plus eriochrome block T along with at least one of 2,2′-bipyridyl, 1,10-phenanthroline, and 2,9-dimethyl-1,10-phenanthroline

The invention relates to a base sequence for coding nitrilase of alcaligenes, relating to a carrier comprising the base sequence and a transformant comprising the base sequence or the carrier, wherein the carrier comprises the base sequence, or a composite unit connecting the base sequence with a corresponding signal sequence. The invention also relates to an amino acid sequence coded by the basesequence, and further relates to a technical method for preparing single enantiomorph of mandelic acid and analogue thereof from corresponding mandelonitrile by utilizing the recombinant expression nitrilase as catalyst.

A method for producing a compound represented by the following formula (2)(wherein, X, R1 and m represent respectively the same meaning as described below)which comprises reacting a compound represented by the following formula (1)(wherein, X represents an optionally substituted alkyl group or the like. R1 represents an optionally substituted alkyl group or the like. m represents an integer of 0 to 4. When m represents an integer of 2 to 4, R1s may be the same or different from each other)with hydrogen cyanide in an amount of 1.2 to 5 mol with respect to 1 mol of the compound, in the presence of an organic base, in an organic solvent.

The invention provides a method for producing R-mandelic acid and derivates thereof by biocatalysis. The method comprises performing hydrolysis reaction in a reaction system which takes racemization mandelonitrile compound shown in formula (I) as the zymolyte, and nitrilase obtained by culturing bacillus foecalis alkaligenes (CCTCC No: M 208168) as the catalyst at 20-60 DEG C with pH being 8.0-8.5 to obtain chiral R-mandelic acid and derivates thereof shown in corresponding formula (II). The invention has the beneficial effects that the activity of strain bacillus foecalis alkaligenes is high, the dosage of thallus is little, the produced waste water in the reaction process is little, and pollution is less; the reaction conditions are mild and energy consumption is low; the cost is low, conversion rate is high and yield is high, and the invention is favorable for industrialized production of chiral R-mandelic acid and R-chloro mandelic acid.

The invention provides a method for producing R-mandelic acid with biocatalysis and separating and coupling method. The method comprises: adding alkaliteit anion exchange resin of 10-50g / L into a reaction system with racemic mandelonitrile as substrate and nitrilase as catalyst after reaction begins 0-1 hour to perform hydrolysis reaction with pH being 8.0-8.5 and temperature being 20-60 DEG C; when the concentration of substrate racemic mandelonitrile in the conversion system is low, further adding racemic mandelonitrile of 10-100mmol / L and alkaliteit anion exchange resin of 10-50g / L into the reaction system to perform hydrolysis reaction of next batch with the same conditions. The invention provides a method for efficiently producing R-mandelic acid, R-mandelic acid is prepared by nitrilase biocatalysis and separating and coupling technology, thus effectively removing inhibitory action of the product; resin is used for absorbing R-mandelic acid to perform coupling conversion, thus the catalysis yield of the nitrilase biocatalyst is greatly enhanced and sequential separation is facilitated; therefore, the inventive technology has good industrial application prospect.

The invention discloses a new nitrilase and gene thereof, a recombinant expression vector and a recombinant expression transformant containing the gene, a method for preparing the recombinant nitrilase or microbial cell containing the recombinant nitrilase by use of the recombinant expression transformant, and application of the microbial cell in dehydrating ortho-chlorine mandelonitrile or other analogues and producing chiral ortho-chlorine mandelonitrile or other analogues. The recombinant nitrilase disclosed by the invention comes from Labrenzia aggregate and can be used as a catalyst for dehydrating and splitting ortho-chlorine mandelonitrile or other analogues; and the recombinant nitrilase has the advantages of high catalysis efficiency, strong enantioselectivity, mild reaction conditions, environmental friendliness and the like.

The invention discloses a method for preparing R-mandelic acid, and belongs to the technical field of preparation of mandelic acid. Commercial racemic mandelonitrile, immobilized nitrilase and commercial macroporous strongly-basic anion resin are used as raw materials and catalyzed to generate the R-mandelic acid; and the R-mandelic acid is obtained by elution, concentration, drying and crystallization. The optical purity of the R-mandelic acid product prepared by the method reaches 98 percent, the R-mandelic acid can be widely applied to synthesis of multiple medicinal intermediates, the yield of the R-mandelic acid reaches over 70 percent, and the utilization rate of the raw materials is high; and the production equipment is conventional, the reaction conditions are mild, material resources are fully utilized, 'three wastes' are not discharged, the production cost is low, and the method is favorable for environmental protection and is an environment-friendly method. The product prepared by the method can be widely applied in the industries of medicine, hygiene, dyes, health care and the like.

The invention belongs to the synthetic field of organic chemistry, and is particularly relates to a method for synthesizing diphenylacetonitrile by using mandelonitrile. The method comprises the steps of adding mandelonitrile and benzene into a reactor with a stirrer, a condenser and a thermometer; dropwise adding concentrated sulfuric acid at a temperature lower than 45 DEG C; reacting for 1 hour after concentrated sulfuric acid is completely added; adding water; layering; washing the oil layer with water; drying and distilling out benzene; and distilling out a product at a reduced pressure. The method synthesizes diphenylacetonitrile by using mandelonitrile, thereby preventing strong tearing property of alpha-bromobenzyl cyanide which is an intermediate prepared by using bromine. Besides, the method takes use of concentrated sulfuric acid as a condensing agent, thereby reducing cost.